{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T14:35:04Z","timestamp":1771511704145,"version":"3.50.1"},"reference-count":35,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T00:00:00Z","timestamp":1707696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81660265"],"award-info":[{"award-number":["81660265"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Applied Research and Cultivation Program of Jiangxi Province","award":["20212BAG70049"],"award-info":[{"award-number":["20212BAG70049"]}]},{"name":"The Key Research and Development Program of Jiangxi Province","award":["20202BBGL73065"],"award-info":[{"award-number":["20202BBGL73065"]}]},{"name":"The Natural Science Foundation of Jiangxi Province","award":["20224BAB206026"],"award-info":[{"award-number":["20224BAB206026"]}]},{"name":"The Natural Science Foundation of Jiangxi Province","award":["20181BAB205015"],"award-info":[{"award-number":["20181BAB205015"]}]},{"name":"The Science and Technology Plan of Jiangxi Provincial Health Commission","award":["202130758"],"award-info":[{"award-number":["202130758"]}]},{"name":"The Science and Technology Plan of Jiangxi Provincial Health Commission","award":["202210060"],"award-info":[{"award-number":["202210060"]}]},{"DOI":"10.13039\/501100004761","name":"The Natural Science Foundation of Hunan Province","doi-asserted-by":"crossref","award":["2019JJ50955"],"award-info":[{"award-number":["2019JJ50955"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Jiangxi Maternal and Child Health Hospital"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Mesenchymal stem cells play important roles in repairing injured endometrium. However, the molecular targets and potential mechanism of the endometrial recipient cells for stem cell therapy in intrauterine adhesion (IUA) are poorly understood. In this study, umbilical cord mesenchymal stem-cell-conditioned medium (UCMSCs-CM) produced positive effects on a Transforming growth factor beta (TGF-\u03b2) induced IUA cell model. RNA-sequencing was performed on clinical IUA tissues, and the top 40 upregulated and top 20 downregulated mRNAs were selected and verified using high-throughput (HT) qPCR in both tissues and cell models. Based on a bioinformatic analysis of RNA-sequencing and HT-qPCR results, 11 mRNAs were uncovered to be the intervention targets of UCMSCs-CM on IUA endometrium cell models. Among them, IGFBP3 was striking as a key pathogenic gene and a potential diagnostic marker of IUA, which exhibited the area under the curve (AUC), sensitivity, specificity were 0.924, 93.1% and 80.6%, respectively in 60 endometrial tissues. The silencing of IGFBP3 exerted positive effects on the IUA cell model through partially upregulating MMP1 and KLF2. In conclusion, RNA-sequencing combined with HT qPCR based on clinical tissues and IUA cell models were used in IUA research and our results may provide some scientific ideas for the diagnosis and treatment of IUA.<\/jats:p>","DOI":"10.1242\/bio.060141","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T10:40:38Z","timestamp":1705315238000},"source":"Crossref","is-referenced-by-count":4,"title":["Novel therapeutic targets, including IGFBP3, of umbilical cord mesenchymal stem-cell-conditioned medium in intrauterine adhesion"],"prefix":"10.1242","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2838-9359","authenticated-orcid":false,"given":"Yuan","family":"Zhu","sequence":"first","affiliation":[{"name":"Jiangxi Maternal and Child Health Hospital 1 Department of Gynecology , , Nanchang, 330000 , China"},{"name":"Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College 2 Department of Obstetrics and Gynecology , , Nanchang, 330000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2243-6193","authenticated-orcid":false,"given":"Mingjie","family":"Bao","sequence":"additional","affiliation":[{"name":"Jiangxi Maternal and Child Health Hospital 1 Department of Gynecology , , Nanchang, 330000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2484-8095","authenticated-orcid":false,"given":"Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangxi Maternal and Child Health Hospital 1 Department of Gynecology , , Nanchang, 330000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8329-4272","authenticated-orcid":false,"given":"Xiaoyan","family":"Ai","sequence":"additional","affiliation":[{"name":"Jiangxi Maternal and Child Health Hospital 1 Department of Gynecology , , Nanchang, 330000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6156-2344","authenticated-orcid":false,"given":"Dewen","family":"Qiu","sequence":"additional","affiliation":[{"name":"Jiangxi Maternal and Child Health Hospital 3 Clinical laboratory , , Nanchang, 330000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4388-0218","authenticated-orcid":false,"given":"Changhua","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangxi Maternal and Child Health Hospital 1 Department of Gynecology , , Nanchang, 330000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,2,12]]},"reference":[{"key":"2024021214281720900_BIO060141C1","doi-asserted-by":"publisher","first-page":"e13262","DOI":"10.1111\/aji.13262","article-title":"Transforming growth factor-beta1 in intrauterine adhesion","volume":"84","author":"Abudukeyoumu","year":"2020","journal-title":"Am. 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