{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:02:02Z","timestamp":1790445722625,"version":"4.1.0"},"reference-count":71,"publisher":"The Company of Biologists","issue":"12","license":[{"start":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100021710","name":"Greater New Orleans Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100021710","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009723","name":"University of New Orleans","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009723","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The hypoxia inducible factor 1 (HIF1) is a central regulator of the molecular responses of animals to low oxygen. While the hypoxia-responsiveness of HIF1 is generally attributed to the stabilization of the alpha protein subunit (HIF1\u03b1) at low oxygen, several studies on fish report increased tissue levels of HIF1A mRNA during hypoxia, suggesting transcriptional regulation. In the current study, HIF1\u03b1 protein and HIF1A mRNA were determined in parallel in tissues of Gulf killifish, Fundulus grandis, exposed to short-term hypoxia (24\u2005h at 1\u2005mg\u2005O2\u00a0l\u22121). HIF1\u03b1 protein was higher in brain, ovary, and skeletal muscle from fish exposed to hypoxia compared with normoxic controls by 6\u2005h, and it remained elevated in brain and ovary at 24\u2005h. In contrast, HIF1A mRNA levels were unaffected by hypoxia in any tissue. Moreover, HIF1\u03b1 protein and HIF1A mRNA levels in the same tissues were not correlated with one another during either normoxia or hypoxia. Hence, an increase in HIF1\u03b1 protein does not depend upon an increase in HIF1A mRNA during acute exposure to low oxygen in this species. The results support the widely accepted mechanism of post-translational protein stabilization, rather than new transcription, during the initial response of fish to hypoxia.<\/jats:p>","DOI":"10.1242\/bio.060167","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T08:13:26Z","timestamp":1703060006000},"source":"Crossref","is-referenced-by-count":14,"title":["Hypoxia-inducible factor 1 alpha protein increases without changes in mRNA during acute hypoxic exposure of the Gulf killifish,\n                    <i>Fundulus grandis<\/i>"],"prefix":"10.1242","volume":"12","author":[{"given":"Taylor E.","family":"Murphy","sequence":"first","affiliation":[{"name":"University of New Orleans Department of Biological Sciences , , New Orleans, LA, 70148 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine C.","family":"Harris","sequence":"additional","affiliation":[{"name":"University of New Orleans Department of Biological Sciences , , New Orleans, LA, 70148 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5636-1700","authenticated-orcid":false,"given":"Bernard B.","family":"Rees","sequence":"additional","affiliation":[{"name":"University of New Orleans Department of Biological Sciences , , New Orleans, LA, 70148 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,12,27]]},"reference":[{"key":"2023122712040621900_BIO060167C1","doi-asserted-by":"publisher","first-page":"268","DOI":"10.3390\/ijms22010268","article-title":"The role of hypoxia-inducible factor post-translational modifications in regulating its localisation, stability, and activity","volume":"22","author":"Albanese","year":"2020","journal-title":"Int. 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