{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T10:47:21Z","timestamp":1786618041676,"version":"build-2736575974"},"reference-count":43,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T00:00:00Z","timestamp":1713830400000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100002770","name":"Cabinet Office, Government of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002770","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007173","name":"Bio-oriented Technology Research Advancement Institution","doi-asserted-by":"crossref","award":["JPJ009237"],"award-info":[{"award-number":["JPJ009237"]}],"id":[{"id":"10.13039\/501100007173","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Waseda University: Waseda Daigaku"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Circadian rhythms are indispensable intrinsic programs that regulate the daily rhythmicity of physiological processes, such as feeding and sleep. The cricket has been employed as a model organism for understanding the neural mechanisms underlying circadian rhythms in insects. However, previous studies measuring rhythm-controlled behaviours only analysed locomotive activity using seesaw-type and infrared sensor-based actometers. Meanwhile, advances in deep learning techniques have made it possible to analyse animal behaviour and posture using software that is devoid of human bias and does not require physical tagging of individual animals. Here, we present a system that can simultaneously quantify multiple behaviours in individual crickets \u2013 such as locomotor activity, feeding, and sleep-like states \u2013 in the long-term, using DeepLabCut, a supervised machine learning-based software for body keypoints labelling. Our system successfully labelled the six body parts of a single cricket with a high level of confidence and produced reliable data showing the diurnal rhythms of multiple behaviours. Our system also enabled the estimation of sleep-like states by focusing on posture, instead of immobility time, which is a conventional parameter. We anticipate that this system will provide an opportunity for simultaneous and automatic prediction of cricket behaviour and posture, facilitating the study of circadian rhythms.<\/jats:p>","DOI":"10.1242\/bio.060237","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T08:30:56Z","timestamp":1711528256000},"source":"Crossref","is-referenced-by-count":9,"title":["DeepLabCut-based daily behavioural and posture analysis in a cricket"],"prefix":"10.1242","volume":"13","author":[{"given":"Shota","family":"Hayakawa","sequence":"first","affiliation":[{"name":"Department of Advanced Science and Engineering, Graduate School of Advanced Science and Engineering, Waseda University 1 , Tokyo 162-8480 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6023-7710","authenticated-orcid":false,"given":"Kosuke","family":"Kataoka","sequence":"additional","affiliation":[{"name":"Comprehensive Research Organization, Waseda University 2 , Tokyo 162-8480 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0920-0562","authenticated-orcid":false,"given":"Masanobu","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Division of Sciences for Biological System, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology 3 , Tokyo 184-8588 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Asahi","sequence":"additional","affiliation":[{"name":"Department of Advanced Science and Engineering, Graduate School of Advanced Science and Engineering, Waseda University 1 , Tokyo 162-8480 , Japan"},{"name":"Comprehensive Research Organization, Waseda University 2 , Tokyo 162-8480 , Japan"},{"name":"Research Organization for Nano & Life Innovation, Waseda University 4 , Tokyo 162-0041 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7930-1425","authenticated-orcid":false,"given":"Takeshi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Division of Sciences for Biological System, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology 3 , Tokyo 184-8588 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,4,23]]},"reference":[{"key":"2024042308012370600_BIO060237C1","doi-asserted-by":"publisher","first-page":"719","DOI":"10.2108\/zsj.14.719","article-title":"Circadian locomotor rhythms in the cricket, Gryllodes sigillatus I. 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