{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:03:33Z","timestamp":1753884213864,"version":"3.41.2"},"reference-count":37,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["Cluster of Excellence 171 \u201cNanoscale Microscopy and Molecular Physiology of the Brain\u201d"],"award-info":[{"award-number":["Cluster of Excellence 171 \u201cNanoscale Microscopy and Molecular Physiology of the Brain\u201d"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008001","name":"University of Cologne: Universit\u00e4t zu K\u00f6ln","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008001","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Bazooka\/Par-3 (Baz) is an evolutionarily conserved scaffold protein that functions as a master regulator for the establishment and maintenance of cell polarity in many different cell types. In the vast majority of published research papers Baz has been reported to localize at the cell cortex and at intercellular junctions. However, there have also been several reports showing localization and function of Baz at additional subcellular sites, in particular the nuclear envelope and the neuromuscular junction. In this study we have re-assessed the localization of Baz to these subcellular sites in a systematic manner. We used antibodies raised in different host animals against different epitopes of Baz for confocal imaging of Drosophila tissues. We tested the specificity of these antisera by mosaic analysis with null mutant baz alleles and tissue-specific RNAi against baz. In addition, we used a GFP-tagged gene trap line for Baz and a bacterial artificial chromosome (BAC) expressing GFP-tagged Baz under control of its endogenous promoter in a baz mutant background to compare the subcellular localization of the GFP-Baz fusion proteins to the staining with anti-Baz antisera. Together, these experiments did not provide evidence for specific localization of Baz to the nucleus or the neuromuscular junction.<\/jats:p>","DOI":"10.1242\/bio.060544","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T09:42:46Z","timestamp":1717666966000},"source":"Crossref","is-referenced-by-count":0,"title":["Re-assessment of the subcellular localization of Bazooka\/Par-3 in <i>Drosophila<\/i>: no evidence for localization to the nucleus and the neuromuscular junction"],"prefix":"10.1242","volume":"13","author":[{"given":"Soya","family":"Kim","sequence":"first","affiliation":[{"name":"Center for Anatomy, University of Cologne and University Hospital Cologne 1 Molecular Cell Biology , , Weyertal 115c, 50931 K\u00f6ln , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaffer","family":"Shahab","sequence":"additional","affiliation":[{"name":"Institute for Anatomy and Cell Biology, Georg-August-University G\u00f6ttingen 2 Stem Cell Biology , , Justus-von-Liebig-Weg 11, 37077 G\u00f6ttingen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Vogelsang","sequence":"additional","affiliation":[{"name":"Center for Anatomy, University of Cologne and University Hospital Cologne 1 Molecular Cell Biology , , Weyertal 115c, 50931 K\u00f6ln , Germany"},{"name":"Center for Molecular Medicine Cologne, University of Cologne and University Hospital Cologne 3 , Robert-Koch-Str. 21, 50931 Cologne , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0007-6289","authenticated-orcid":false,"given":"Andreas","family":"Wodarz","sequence":"additional","affiliation":[{"name":"Center for Anatomy, University of Cologne and University Hospital Cologne 1 Molecular Cell Biology , , Weyertal 115c, 50931 K\u00f6ln , Germany"},{"name":"Institute for Anatomy and Cell Biology, Georg-August-University G\u00f6ttingen 2 Stem Cell Biology , , Justus-von-Liebig-Weg 11, 37077 G\u00f6ttingen , Germany"},{"name":"Center for Molecular Medicine Cologne, University of Cologne and University Hospital Cologne 3 , Robert-Koch-Str. 21, 50931 Cologne , Germany"},{"name":"Cluster of Excellence - Cellular stress response in aging-associated diseases (CECAD), University of Cologne and University Hospital Cologne 4 , Joseph-Stelzmann-Str. 26, 50931 Cologne , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"2024062709122465600_BIO060544C1","doi-asserted-by":"publisher","first-page":"1833","DOI":"10.1242\/dev.047647","article-title":"PAR-3 mediates the initial clustering and apical localization of junction and polarity proteins during C. elegans intestinal epithelial cell polarization","volume":"137","author":"Achilleos","year":"2010","journal-title":"Development"},{"key":"2024062709122465600_BIO060544C2","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1016\/j.cub.2015.02.073","article-title":"Planar cell polarity breaks the symmetry of PAR protein distribution prior to mitosis in drosophila sensory organ precursor cells","volume":"25","author":"Besson","year":"2015","journal-title":"Curr. 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