{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:03:47Z","timestamp":1772168627037,"version":"3.50.1"},"reference-count":22,"publisher":"The Company of Biologists","issue":"11","license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["GM127938"],"award-info":[{"award-number":["GM127938"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007188","name":"Uniformed Services University of the Health Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007188","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Drosophila oogenesis has long been an important model for understanding myriad cellular processes controlling development, RNA biology and patterning. Flies are easily fed drugs to disrupt various molecular pathways. However, this is often done under poor nutrient conditions that adversely affect oogenesis, thus making analysis challenging. Cycloheximide is a widely used compound that binds to and stalls the ribosome, therefore reducing protein synthesis. As egg production is a highly nutrient-dependent process, we developed a method to feed female Drosophila a rich diet of yeast paste supplemented with cycloheximide to better determine the effect of cycloheximide treatment on oogenesis. We found that flies readily consumed cycloheximide-supplemented yeast paste. Males and females had reduced lifespans when maintained on cycloheximide, with males exhibiting a dose-dependent decrease. Although females did not exhibit decreased egg laying, their ovaries were smaller and the number of progeny reduced, indicating substandard egg quality. Finally, females fed cycloheximide had disrupted oogenesis, with smaller ovaries, missing ovariole stages, and an increase in apoptotic follicles. Together, these data support that reduced protein synthesis adversely affects oogenesis with a rich diet that provides optimal nutrient conditions. In addition, this method could be used more broadly to test the effect of other drugs on Drosophila oogenesis without the confounding effects caused by poor nutrition.<\/jats:p>","DOI":"10.1242\/bio.061697","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T04:38:49Z","timestamp":1732595929000},"source":"Crossref","is-referenced-by-count":1,"title":["Feeding a rich diet supplemented with the translation inhibitor cycloheximide decreases lifespan and ovary size in\n                    <i>Drosophila<\/i>"],"prefix":"10.1242","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1083-5143","authenticated-orcid":false,"given":"Hye Jin","family":"Hwang","sequence":"first","affiliation":[{"name":"Uniformed Services University 1 Department of Biochemistry and Molecular Biology , , Bethesda, MD 20814 ,","place":["USA"]},{"name":"Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. 2 , Bethesda, MD 20817 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1945-0541","authenticated-orcid":false,"given":"Rachel T.","family":"Cox","sequence":"additional","affiliation":[{"name":"Uniformed Services University 1 Department of Biochemistry and Molecular Biology , , Bethesda, MD 20814 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,11,25]]},"reference":[{"key":"2024112609381561900_BIO061697C1","doi-asserted-by":"publisher","first-page":"R69","DOI":"10.1530\/REP-18-0593","article-title":"Drosophila melanogaster as a model for nutrient regulation of ovarian function","volume":"159","author":"Armstrong","year":"2020","journal-title":"Reproduction"},{"key":"2024112609381561900_BIO061697C2","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1128\/MCB.00824-13","article-title":"In vivo interaction proteomics reveal a novel p38 mitogen-activated protein kinase\/Rack1 pathway regulating proteostasis in Drosophila muscle","volume":"34","author":"Belozerov","year":"2014","journal-title":"Mol. 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