{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:04:02Z","timestamp":1772168642690,"version":"3.50.1"},"reference-count":72,"publisher":"The Company of Biologists","issue":"12","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004377","name":"The Hong Kong Polytechnic University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004377","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The University of Melbourne School of BioSciences"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Myopia (short-sightedness) is the most common ocular disorder. It generally develops after over-exposure to aberrant visual environments, disrupting emmetropization mechanisms that should match eye growth with optical power. A pre-screening of strongly associated myopia-risk genes identified through human genome-wide association studies implicates efemp1 in myopia development, but how this gene impacts ocular growth remains unclear. Here, we modify efemp1 expression specifically in the retina of zebrafish. We found that under normal lighting, efemp1 mutants developed axial myopia, enlarged eyes, reduced spatial vision and altered retinal function. However, under myopia-inducing dark-rearing, compared to control fish, mutants remained emmetropic and showed changes in retinal function. Efemp1 modification changed the expression of efemp1, egr1, tgfb1a, vegfab and rbp3 genes in the eye, and changed the inner retinal distributions of myopia-associated EFEMP1, TIMP2 and MMP2 proteins. Efemp1 modification also impacted dark-rearing-induced responses of vegfab and wnt2b genes and above-mentioned myopia-associated proteins. Together, we provided robust evidence that light-dependent ocular growth is regulated by efemp1.<\/jats:p>","DOI":"10.1242\/bio.061741","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T06:08:08Z","timestamp":1732774088000},"source":"Crossref","is-referenced-by-count":1,"title":["EFEMP1 contributes to light-dependent ocular growth in zebrafish"],"prefix":"10.1242","volume":"13","author":[{"given":"Jiaheng","family":"Xie","sequence":"first","affiliation":[{"name":"School of BioSciences, The University of Melbourne 1 , Parkville, Victoria, 3010 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bang V.","family":"Bui","sequence":"additional","affiliation":[{"name":"Department of Optometry and Vision Sciences, The University of Melbourne 2 , Parkville, Victoria, 3010 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick T.","family":"Goodbourn","sequence":"additional","affiliation":[{"name":"Melbourne School of Psychological Sciences, The University of Melbourne 3 , Parkville, Victoria, 3010 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0747-4084","authenticated-orcid":false,"given":"Patricia R.","family":"Jusuf","sequence":"additional","affiliation":[{"name":"School of BioSciences, The University of Melbourne 1 , Parkville, Victoria, 3010 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,11,28]]},"reference":[{"key":"2024112811073232400_BIO061741C1","doi-asserted-by":"publisher","first-page":"2621","DOI":"10.1158\/0008-5472.Can-04-4096","article-title":"Fibulins 3 and 5 antagonize tumor angiogenesis in vivo","volume":"66","author":"Albig","year":"2006","journal-title":"Cancer Res."},{"key":"2024112811073232400_BIO061741C2","first-page":"471","article-title":"The FGF2 gene in a myopia animal model and human subjects","volume":"18","author":"An","year":"2012","journal-title":"Mol. 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