{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:03:40Z","timestamp":1772168620551,"version":"3.50.1"},"reference-count":45,"publisher":"The Company of Biologists","issue":"8","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R35GM122492"],"award-info":[{"award-number":["R35GM122492"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R01HD092990"],"award-info":[{"award-number":["R01HD092990"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007277","name":"Icahn School of Medicine at Mount Sinai","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007277","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Diversin is a vertebrate homolog of the core planar cell polarity (PCP) protein Diego. Here we studied the function of Diversin in Xenopus embryo morphogenesis and its subcellular localization at different locations in superficial ectoderm cells. Depletion of Diversin in the neuroectoderm inhibited apical domain size and neural tube closure and disrupted the polarized localization of endogenous Vangl2, another PCP protein. Whereas Diversin puncta were randomly distributed in early ectoderm, they acquired planar polarity in the neuroectoderm in a stage- and position-specific manner. We find that Diversin is accumulated at the cell junctions adjacent to apically constricting cells at the Xenopus neural plate border and the gastrula blastopore lip. Moreover, Diversin cytoplasmic puncta redistributed in the direction of the pulling forces from the cells with constricting apical domains, suggesting a mechanosensitive process. PCP complexes of Dishevelled (Dvl2) and Diversin or the mechanosensitive adaptor ADIP exhibited planar polarity in the neural plate and the wound edge and promoted wound healing. We propose that Diversin- and Dvl2-containing PCP complexes control morphogenesis in a tension-dependent manner.<\/jats:p>","DOI":"10.1242\/bio.062128","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T14:03:09Z","timestamp":1753711389000},"source":"Crossref","is-referenced-by-count":3,"title":["Mechanosensitive localization of Diversin highlights its function in vertebrate morphogenesis and planar cell polarity"],"prefix":"10.1242","volume":"14","author":[{"given":"Satheeja Santhi","family":"Velayudhan","sequence":"first","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai Department of Cell, Developmental and Regenerative Biology , , New York, NY 10029 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih-Wen","family":"Chu","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai Department of Cell, Developmental and Regenerative Biology , , New York, NY 10029 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Itoh","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai Department of Cell, Developmental and Regenerative Biology , , New York, NY 10029 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3963-9202","authenticated-orcid":false,"given":"Sergei Y.","family":"Sokol","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai Department of Cell, Developmental and Regenerative Biology , , New York, NY 10029 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2025,8,11]]},"reference":[{"key":"2025101403114127900_BIO062128C1","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1002\/bdra.23273","article-title":"Genetic studies of ANKRD6 as a molecular switch between Wnt signaling pathways in human neural tube defects","volume":"103","author":"Allache","year":"2015","journal-title":"Birth Defects Res. A Clin. Mol. Teratol"},{"key":"2025101403114127900_BIO062128C2","doi-asserted-by":"publisher","first-page":"4103","DOI":"10.1074\/jbc.M209832200","article-title":"ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions","volume":"278","author":"Asada","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2025101403114127900_BIO062128C3","doi-asserted-by":"publisher","first-page":"1182","DOI":"10.1101\/gad.890501","article-title":"Unipolar membrane association of Dishevelled mediates Frizzled planar cell polarity signaling","volume":"15","author":"Axelrod","year":"2001","journal-title":"Genes Dev."},{"key":"2025101403114127900_BIO062128C4","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1038\/nrm.2017.11","article-title":"Planar cell polarity in development and disease","volume":"18","author":"Butler","year":"2017","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2025101403114127900_BIO062128C5","doi-asserted-by":"publisher","first-page":"2774","DOI":"10.1016\/j.cub.2015.09.015","article-title":"Mechanical strain determines the axis of planar polarity in ciliated epithelia","volume":"25","author":"Chien","year":"2015","journal-title":"Curr. Biol."},{"key":"2025101403114127900_BIO062128C6","doi-asserted-by":"publisher","first-page":"e16463","DOI":"10.7554\/eLife.16463","article-title":"Wnt proteins can direct planar cell polarity in vertebrate ectoderm","volume":"5","author":"Chu","year":"2016","journal-title":"eLife"},{"key":"2025101403114127900_BIO062128C7","doi-asserted-by":"publisher","first-page":"3315","DOI":"10.1016\/j.cub.2025.05.069","article-title":"Mechanical cues polarize ADIP protein complexes to control vertebrate morphogenesis and wound healing","volume":"35","author":"Chu","year":"2025","journal-title":"Curr. Biol."},{"key":"2025101403114127900_BIO062128C8","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1038\/nature04375","article-title":"Planar cell polarity signalling couples cell division and morphogenesis during neurulation","volume":"439","author":"Ciruna","year":"2006","journal-title":"Nature"},{"key":"2025101403114127900_BIO062128C9","doi-asserted-by":"publisher","first-page":"4467","DOI":"10.1242\/dev.01317","article-title":"Diego interacts with Prickle and Strabismus\/Van Gogh to localize planar cell polarity complexes","volume":"131","author":"Das","year":"2004","journal-title":"Development"},{"key":"2025101403114127900_BIO062128C10","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1038\/ncb1784","article-title":"Planar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles","volume":"10","author":"Devenport","year":"2008","journal-title":"Nat. Cell Biol."},{"key":"2025101403114127900_BIO062128C11","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/S1534-5807(01)00010-7","article-title":"The ankyrin repeat protein Diego mediates Frizzled-dependent planar polarization","volume":"1","author":"Feiguin","year":"2001","journal-title":"Dev. Cell"},{"key":"2025101403114127900_BIO062128C12","doi-asserted-by":"publisher","first-page":"1877","DOI":"10.1242\/dev.054080","article-title":"Principles of planar polarity in animal development","volume":"138","author":"Goodrich","year":"2011","journal-title":"Development"},{"key":"2025101403114127900_BIO062128C13","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.devcel.2011.06.011","article-title":"Planar cell polarity: coordinating morphogenetic cell behaviors with embryonic polarity","volume":"21","author":"Gray","year":"2011","journal-title":"Dev. Cell"},{"key":"2025101403114127900_BIO062128C14","doi-asserted-by":"publisher","first-page":"2125","DOI":"10.1016\/j.cub.2003.11.054","article-title":"Shroom induces apical constriction and is required for hingepoint formation during neural tube closure","volume":"13","author":"Haigo","year":"2003","journal-title":"Curr. Biol."},{"key":"2025101403114127900_BIO062128C15","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1016\/S0092-8674(00)81537-8","article-title":"Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice","volume":"99","author":"Hildebrand","year":"1999","journal-title":"Cell"},{"key":"2025101403114127900_BIO062128C16","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1002\/embr.201337929","article-title":"Msd1\/SSX2IP-dependent microtubule anchorage ensures spindle orientation and primary cilia formation","volume":"15","author":"Hori","year":"2014","journal-title":"EMBO Rep."},{"key":"2025101403114127900_BIO062128C17","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/jbiol20","article-title":"Nuclear localization is required for Dishevelled function in Wnt\/beta-catenin signaling","volume":"4","author":"Itoh","year":"2005","journal-title":"J. Biol."},{"key":"2025101403114127900_BIO062128C18","doi-asserted-by":"publisher","first-page":"3791","DOI":"10.1242\/jcs.057067","article-title":"Centrosomal localization of Diversin and its relevance to Wnt signaling","volume":"122","author":"Itoh","year":"2009","journal-title":"J. Cell Sci."},{"key":"2025101403114127900_BIO062128C19","doi-asserted-by":"publisher","first-page":"102520","DOI":"10.1016\/j.isci.2021.102520","article-title":"Pinhead antagonizes Admp to promote notochord formation","volume":"24","author":"Itoh","year":"2021","journal-title":"iScience"},{"key":"2025101403114127900_BIO062128C20","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1038\/ncb1271","article-title":"Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding","volume":"7","author":"Jenny","year":"2005","journal-title":"Nat. Cell Biol."},{"key":"2025101403114127900_BIO062128C21","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.ydbio.2014.08.029","article-title":"Ankrd6 is a mammalian functional homolog of Drosophila planar cell polarity gene diego and regulates coordinated cellular orientation in the mouse inner ear","volume":"395","author":"Jones","year":"2014","journal-title":"Dev. Biol."},{"key":"2025101403114127900_BIO062128C22","doi-asserted-by":"publisher","first-page":"bio058761","DOI":"10.1242\/bio.058761","article-title":"The dorsal blastopore lip is a source of signals inducing planar cell polarity in the Xenopus neural plate","volume":"10","author":"Mancini","year":"2021","journal-title":"Biol. Open"},{"key":"2025101403114127900_BIO062128C23","doi-asserted-by":"publisher","first-page":"1987","DOI":"10.1242\/dev.102228","article-title":"Apical constriction: themes and variations on a cellular mechanism driving morphogenesis","volume":"141","author":"Martin","year":"2014","journal-title":"Development"},{"key":"2025101403114127900_BIO062128C24","doi-asserted-by":"publisher","first-page":"8475","DOI":"10.1038\/s41467-023-43973-x","article-title":"Mechanical control of neural plate folding by apical domain alteration","volume":"14","author":"Matsuda","year":"2023","journal-title":"Nat. Commun."},{"key":"2025101403114127900_BIO062128C25","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1083\/jcb.146.2.427","article-title":"Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation","volume":"146","author":"Miller","year":"1999","journal-title":"J. Cell Biol."},{"key":"2025101403114127900_BIO062128C26","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1038\/26006","article-title":"Cloning of inv, a gene that controls left\/right asymmetry and kidney development","volume":"395","author":"Mochizuki","year":"1998","journal-title":"Nature"},{"key":"2025101403114127900_BIO062128C27","doi-asserted-by":"publisher","first-page":"15900","DOI":"10.1073\/pnas.0603808103","article-title":"Diversin regulates heart formation and gastrulation movements in development","volume":"103","author":"Moeller","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025101403114127900_BIO062128C28","volume-title":"Normal Table of Xenopus laevis (Daudin): A Systematical and Chronological Survey of the Development from the Fertilized Egg till the End of Metamorphosis","author":"Nieuwkoop","year":"1994"},{"key":"2025101403114127900_BIO062128C29","doi-asserted-by":"publisher","first-page":"3734","DOI":"10.1038\/ncomms4734","article-title":"Role of Rab11 in planar cell polarity and apical constriction during vertebrate neural tube closure","volume":"5","author":"Ossipova","year":"2014","journal-title":"Nat. Commun."},{"key":"2025101403114127900_BIO062128C30","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1242\/dev.111161","article-title":"Vangl2 cooperates with Rab11 and Myosin V to regulate apical constriction during vertebrate gastrulation","volume":"142","author":"Ossipova","year":"2015","journal-title":"Development"},{"key":"2025101403114127900_BIO062128C31","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1242\/bio.201511676","article-title":"Planar polarization of Vangl2 in the vertebrate neural plate is controlled by Wnt and Myosin II signaling","volume":"4","author":"Ossipova","year":"2015","journal-title":"Biol. Open"},{"key":"2025101403114127900_BIO062128C32","doi-asserted-by":"publisher","first-page":"dev168922","DOI":"10.1242\/dev.168922","article-title":"The RhoGEF protein Plekhg5 regulates apical constriction of bottle cells during gastrulation","volume":"145","author":"Popov","year":"2018","journal-title":"Development"},{"key":"2025101403114127900_BIO062128C33","doi-asserted-by":"publisher","first-page":"3927","DOI":"10.1038\/s41467-025-58557-0","article-title":"Junctional force patterning drives both positional order and planar polarity in the auditory epithelia","volume":"16","author":"Prakash","year":"2025","journal-title":"Nat. Commun."},{"key":"2025101403114127900_BIO062128C34","doi-asserted-by":"publisher","first-page":"e0259068","DOI":"10.1371\/journal.pone.0259068","article-title":"Identification of the centrosomal maturation factor SSX2IP as a Wtip-binding partner by targeted proximity biotinylation","volume":"16","author":"Reis","year":"2021","journal-title":"PLoS ONE"},{"key":"2025101403114127900_BIO062128C35","doi-asserted-by":"publisher","first-page":"2073","DOI":"10.1101\/gad.230402","article-title":"The ankyrin repeat protein Diversin recruits Casein kinase Iepsilon to the beta-catenin degradation complex and acts in both canonical Wnt and Wnt\/JNK signaling","volume":"16","author":"Schwarz-Romond","year":"2002","journal-title":"Genes Dev."},{"key":"2025101403114127900_BIO062128C36","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1038\/ng1552","article-title":"Inversin, the gene product mutated in nephronophthisis type II, functions as a molecular switch between Wnt signaling pathways","volume":"37","author":"Simons","year":"2005","journal-title":"Nat. Genet."},{"key":"2025101403114127900_BIO062128C37","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.semcdb.2015.05.002","article-title":"Spatial and temporal aspects of Wnt signaling and planar cell polarity during vertebrate embryonic development","volume":"42","author":"Sokol","year":"2015","journal-title":"Semin. Cell Dev. Biol."},{"key":"2025101403114127900_BIO062128C38","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1038\/s41592-020-01018-x","article-title":"Cellpose: a generalist algorithm for cellular segmentation","volume":"18","author":"Stringer","year":"2021","journal-title":"Nat. Methods"},{"key":"2025101403114127900_BIO062128C39","doi-asserted-by":"publisher","first-page":"2227","DOI":"10.1242\/dev.127.10.2227","article-title":"Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway","volume":"127","author":"Tada","year":"2000","journal-title":"Development"},{"key":"2025101403114127900_BIO062128C40","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1002\/dvdy.10126","article-title":"Expression of the ankyrin repeat domain 6 gene (Ankrd6) during mouse brain development","volume":"224","author":"Tissir","year":"2002","journal-title":"Dev. Dyn."},{"key":"2025101403114127900_BIO062128C41","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1038\/ncb1593","article-title":"Gamma-tubulin complex-mediated anchoring of spindle microtubules to spindle-pole bodies requires Msd1 in fission yeast","volume":"9","author":"Toya","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"2025101403114127900_BIO062128C42","doi-asserted-by":"publisher","first-page":"2203","DOI":"10.1016\/j.cub.2012.09.046","article-title":"Microtubules enable the planar cell polarity of airway cilia","volume":"22","author":"Vladar","year":"2012","journal-title":"Curr. Biol."},{"key":"2025101403114127900_BIO062128C43","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.ydbio.2025.04.007","article-title":"PCP-dependent polarized mechanics in the cortex of individual cells during convergent extension","volume":"523","author":"Weng","year":"2025","journal-title":"Dev. Biol."},{"key":"2025101403114127900_BIO062128C44","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1016\/j.mod.2011.07.004","article-title":"Regulation of basal body and ciliary functions by Diversin","volume":"128","author":"Yasunaga","year":"2011","journal-title":"Mech. Dev."},{"key":"2025101403114127900_BIO062128C45","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1083\/jcb.200704150","article-title":"Cooperation of polarized cell intercalations drives convergence and extension of presomitic mesoderm during zebrafish gastrulation","volume":"180","author":"Yin","year":"2008","journal-title":"J. Cell Biol."}],"container-title":["Biology Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/bio\/article-pdf\/doi\/10.1242\/bio.062128\/3658712\/bio062128.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/bio\/article-pdf\/doi\/10.1242\/bio.062128\/3660823\/bio062128.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/bio\/article-pdf\/doi\/10.1242\/bio.062128\/3660823\/bio062128.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:11:52Z","timestamp":1760425912000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/bio\/article\/14\/8\/bio062128\/368858\/Mechanosensitive-localization-of-Diversin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,11]]},"references-count":45,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8,15]]}},"URL":"https:\/\/doi.org\/10.1242\/bio.062128","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.06.24.661316","asserted-by":"object"}]},"ISSN":["2046-6390"],"issn-type":[{"value":"2046-6390","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,11]]},"article-number":"bio062128"}}