{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:44:30Z","timestamp":1785833070780,"version":"3.56.0"},"reference-count":19,"publisher":"The Company of Biologists","issue":"7","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Division of Intramural Research","award":["R01HL169511"],"award-info":[{"award-number":["R01HL169511"]}]},{"name":"Division of Intramural Research","award":["R01HL118761"],"award-info":[{"award-number":["R01HL118761"]}]},{"name":"Division of Intramural Research","award":["R01HL127717"],"award-info":[{"award-number":["R01HL127717"]}]},{"name":"Division of Intramural Research","award":["R01HL171574"],"award-info":[{"award-number":["R01HL171574"]}]},{"name":"Division of Intramural Research","award":["R00HL169742"],"award-info":[{"award-number":["R00HL169742"]}]},{"DOI":"10.13039\/100019895","name":"Vivian L. Smith Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100019895","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008102","name":"University of Cincinnati","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008102","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The Fucci mouse lines are valuable tools for tracing cell cycle stages during development and in postnatal tissues; however, the insertion loci for the transgenes remain unidentified. Here, we report the genomic insertion sites of both the Fucci-G1 and -S\/G2\/M transgenes, identified through whole-genome sequencing. Interestingly, the Fucci-G1 transgene is located proximal to the Shh lung gut enhancer (SLGE) on chromosome 5. Homozygous Fucci-G1 embryos are perinatal lethal but survive until embryonic day (E)18.5, exhibiting craniofacial and foregut-related defects. Expression of Shh and canonical targets are downregulated in the pharyngeal arches of E11.5 homozygous Fucci-G1 embryos. Furthermore, in situ hybridization analysis showed diminished Shh expression in the pharyngeal and foregut endoderm, consistent with disruptions to SLGE activity. Our results indicate that heterozygous Fucci-G1 mice are suitable for cell cycle studies; however, we caution potential genetic interference when investigating SHH-related pathways.<\/jats:p>","DOI":"10.1242\/bio.062386","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T07:52:50Z","timestamp":1785829970000},"source":"Crossref","is-referenced-by-count":0,"title":["Fucci-G1 insertion partially disrupts\n                    <i>Shh<\/i>\n                    expression"],"prefix":"10.1242","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4571-8409","authenticated-orcid":false,"given":"Yuka","family":"Morikawa","sequence":"first","affiliation":[{"name":"Texas Heart Institute at Baylor College of Medicine 1 Cardiomyocyte Renewal Lab , , Houston, TX 77030 ,","place":["USA"]},{"name":"Baylor College of Medicine 2 Department of Integrative Physiology , , Houston, TX 77030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Bromley","sequence":"additional","affiliation":[{"name":"Texas Heart Institute at Baylor College of Medicine 1 Cardiomyocyte Renewal Lab , , Houston, TX 77030 ,","place":["USA"]},{"name":"Baylor College of Medicine 2 Department of Integrative Physiology , , Houston, TX 77030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1369-9661","authenticated-orcid":false,"given":"Jeffrey D.","family":"Steimle","sequence":"additional","affiliation":[{"name":"Baylor College of Medicine 2 Department of Integrative Physiology , , Houston, TX 77030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7842-9857","authenticated-orcid":false,"given":"James F.","family":"Martin","sequence":"additional","affiliation":[{"name":"Texas Heart Institute at Baylor College of Medicine 1 Cardiomyocyte Renewal Lab , , Houston, TX 77030 ,","place":["USA"]},{"name":"Baylor College of Medicine 2 Department of Integrative Physiology , , Houston, TX 77030 ,","place":["USA"]},{"name":"Center for Organ Repair and Renewal, Baylor College of Medicine 3 , Houston, TX 77030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"2026080403524063900_BIO062386C1","doi-asserted-by":"publisher","first-page":"3934","DOI":"10.1242\/dev.108480","article-title":"Mapping the Shh long-range regulatory domain","volume":"141","author":"Anderson","year":"2014","journal-title":"Development"},{"key":"2026080403524063900_BIO062386C2","doi-asserted-by":"publisher","first-page":"112880","DOI":"10.1016\/j.yexcr.2021.112880","article-title":"Identification and characterization of distinct cell cycle stages in cardiomyocytes using the FUCCI transgenic system","volume":"408","author":"Baniol","year":"2021","journal-title":"Exp. 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