{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:04:58Z","timestamp":1789376698898,"version":"build-2803163510"},"reference-count":46,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100030692","name":"Intramural Research Program","doi-asserted-by":"crossref","award":["ZIAHD009002-01"],"award-info":[{"award-number":["ZIAHD009002-01"]}],"id":[{"id":"10.13039\/100030692","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Dynamic fibroblast growth factor (FGF)\/extracellular signal-regulated kinase (ERK) signaling plays key roles in development, regeneration, and disease. We recently developed a zebrafish-optimized optogenetic tool, bOpto-FGF, that enables reversible activation of FGF\/ERK signaling in response to blue light (\u223c455\u2005nm) by fusing a zebrafish receptor tyrosine kinase domain to a blue-light-dimerizing light oxygen voltage-sensing domain. Previously, this tool was introduced into zebrafish embryos by mRNA injection. Here, we develop Tg(ubi:bOpto-FGF), a novel transgenic zebrafish ubiquitously expressing bOpto-FGF, to streamline experimental workflows. We demonstrate robust blue-light-mediated activation of FGF\/ERK signaling and FGF target gene expression in early gastrulation-stage Tg(ubi:bOpto-FGF) embryos. Light-mediated signaling activation at this stage is more spatially uniform in transgenics compared to mRNA-injected embryos, and ectopic signaling in response to continuous light exposure is activated within 3\u2005min and maintained for at least 75\u2005min. Furthermore, Tg(ubi:bOpto-FGF) heterozygotes are light responsive from late blastula stages through at least 24\u2005h postfertilization. Finally, we demonstrate light-mediated spatially localized FGF\/ERK signaling activation within 1\u2005min in the head, trunk, and tail at 1\u2005day postfertilization. This transgenic line provides a powerful and convenient new strategy for spatiotemporal manipulation of FGF\/ERK signaling in the vertebrate zebrafish model.<\/jats:p>","DOI":"10.1242\/bio.062695","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:26:01Z","timestamp":1788942361000},"source":"Crossref","is-referenced-by-count":0,"title":["A transgenic zebrafish for spatiotemporal optogenetic activation of FGF\/ERK signaling"],"prefix":"10.1242","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0538-323X","authenticated-orcid":false,"given":"William K.","family":"Anderson","sequence":"first","affiliation":[{"name":"National Institute of Child Health and Human Development, NIH 1 Unit on Developmental Signaling, Division of Developmental Biology, Eunice Kennedy Shriver , Bethesda, MD 20892 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5556-4442","authenticated-orcid":false,"given":"Leanne E.","family":"Iannucci","sequence":"additional","affiliation":[{"name":"National Institute of Biomedical Imaging and Bioengineering, NIH 2 Section on Mechanics and Tissue Remodeling Integrating Computational and Experimental Systems , , Bethesda, MD 20892 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ninet","family":"Sinaii","sequence":"additional","affiliation":[{"name":"Clinical Center, NIH 3 Biostatistics and Clinical Epidemiology Service , , Bethesda, MD 20892 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Porcino","sequence":"additional","affiliation":[{"name":"Clinical Center, NIH 3 Biostatistics and Clinical Epidemiology Service , , Bethesda, MD 20892 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5700-2662","authenticated-orcid":false,"given":"Katherine W.","family":"Rogers","sequence":"additional","affiliation":[{"name":"National Institute of Child Health and Human Development, NIH 1 Unit on Developmental Signaling, Division of Developmental Biology, Eunice Kennedy Shriver , Bethesda, MD 20892 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"2026091404322329400_BIO062695C1","doi-asserted-by":"publisher","DOI":"10.1101\/2025.08.28.672946","article-title":"Tissue-wide, synchronous Erk oscillations time the segmentation of the zebrafish notochord","author":"Adhyapok","year":"2025","journal-title":"bioRxiv"},{"key":"2026091404322329400_BIO062695C2","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1038\/s41580-025-00917-1","article-title":"Signal control during tissue regeneration in adult animals","volume":"27","author":"Bangru","year":"2025","journal-title":"Nat. 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