{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:45:55Z","timestamp":1792788355916,"version":"4.5.0"},"reference-count":68,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Swiss National Science Foundation","award":["212971"],"award-info":[{"award-number":["212971"]}],"id":[{"id":"https:\/\/ror.org\/00yjd3n13","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Centre National de la Recherche Scientifique","id":[{"id":"https:\/\/ror.org\/02feahw73","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Mitochondria power biological processes, driving interest in measuring mitochondrial aerobic metabolism to understand individual variation in performance and fitness. High-resolution respirometry (HRR) is the gold standard because of its sensitivity and versatility, but it is time-consuming and has low throughput, limiting its use in large-scale studies. We assessed the validity of a high-throughput, fluorescence-based plate reader by comparing it to HRR. In a first experiment, we measured mitochondrial respiration in blood cells from two bird species (quails and chickens) across three temperatures (hypothermia, normothermia, and hyperthermia). The two methods were moderately correlated and revealed similar species effects; however, temperature effects differed between approaches, particularly under hyperthermic conditions, indicating that the plate-reader approach is best suited for stable, normothermic conditions. In a second experiment on sex differences in quails at normothermia, the two methods agreed quantitatively for proton leak and oxidative control efficiency and qualitatively for oxidative phosphorylation, although the plate-reader estimate was marginally significant (P=0.060 versus P=0.046 for HRR). The plate reader's main limitation is its lower sensitivity and greater measurement error, which could be offset by increasing sample sizes. Following protocol optimisation for each study system, this approach may offer a useful high-throughput complement to HRR for studies prioritising larger sample sizes.<\/jats:p>","DOI":"10.1242\/bio.062712","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T09:23:53Z","timestamp":1790587433000},"source":"Crossref","is-referenced-by-count":0,"title":["High-throughput mitochondrial aerobic metabolism measurement: assessing a plate-reader approach using avian blood cells"],"prefix":"10.1242","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0123-5648","authenticated-orcid":false,"given":"Cloe","family":"Hadjadji","sequence":"first","affiliation":[{"name":"Swiss Ornithological Institute 1 , Sempach, Seerose 1, CH-6206 ,","place":["Switzerland"]},{"name":"University of Lausanne 2 Department of Ecology and Evolution , , Lausanne CH-1015 ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2303-4723","authenticated-orcid":false,"given":"Molly","family":"Ohse","sequence":"additional","affiliation":[{"name":"Swiss Ornithological Institute 1 , Sempach, Seerose 1, CH-6206 ,","place":["Switzerland"]},{"name":"University of Lausanne 2 Department of Ecology and Evolution , , Lausanne CH-1015 ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5445-5524","authenticated-orcid":false,"given":"Antoine","family":"Stier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, CNRS, IPHC UMR 7178 3 , F-67000 Strasbourg ,","place":["France"]},{"name":"University of Turku 4 Department of Biology , , Turku, FI-20014 ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6759-4371","authenticated-orcid":false,"given":"Pierre","family":"Bize","sequence":"additional","affiliation":[{"name":"Swiss Ornithological Institute 1 , Sempach, Seerose 1, CH-6206 ,","place":["Switzerland"]},{"name":"University of Lausanne 2 Department of Ecology and Evolution , , Lausanne CH-1015 ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"2026101905095698700_BIO062712C1","doi-asserted-by":"publisher","DOI":"10.1201\/9781315735368","volume-title":"Molecular Biology of the Cell","author":"Alberts","year":"2015","edition":"6th edn."},{"key":"2026101905095698700_BIO062712C2","doi-asserted-by":"publisher","first-page":"e63000","DOI":"10.3791\/63000","article-title":"High-resolution respirometry to assess bioenergetics in cells and tissues using chamber- and plate-based respirometers","volume":"176","author":"Awadhpersad","year":"2021","journal-title":"J. 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