{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:30:05Z","timestamp":1791351005624,"version":"4.3.1"},"reference-count":38,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T00:00:00Z","timestamp":1599523200000},"content-version":"vor","delay-in-days":2300,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Hereditary Spastic Paraplegia (HSP) is a genetically heterogeneous group of disorders, diagnosed by progressive gait disturbances with muscle weakness and spasticity, for which there are no treatments targeted at the underlying pathophysiology. Mutations in spastin are a common cause of HSP. Spastin is a microtubule-severing protein whose mutation in mouse causes defective axonal transport. In human patient-derived olfactory neurosphere-derived (ONS) cells, spastin mutations lead to lower levels of acetylated \u03b1-tubulin, a marker of stabilised microtubules, and to slower speed of peroxisome trafficking. Here we screened multiple concentrations of four tubulin-binding drugs for their ability to rescue levels of acetylated \u03b1-tubulin in patient-derived ONS cells. Drug doses that restored acetylated \u03b1-tubulin to levels in control-derived ONS cells were then selected for their ability to rescue peroxisome trafficking deficits. Automated microscopic screening identified very low doses of the four drugs (0.5\u2005nM taxol, 0.5\u2005nM vinblastine, 2\u2005nM epothilone D, 10\u2005\u00b5M noscapine) that rescued acetylated \u03b1-tubulin in patient-derived ONS cells. These same doses rescued peroxisome trafficking deficits, restoring peroxisome speeds to untreated control cell levels. These results demonstrate a novel approach for drug screening based on high throughput automated microscopy for acetylated \u03b1-tubulin followed by functional validation of microtubule-based peroxisome transport. From a clinical perspective, all the drugs tested are used clinically, but at much higher doses. Importantly, epothilone D and noscapine can enter the central nervous system, making them potential candidates for future clinical trials.<\/jats:p>","DOI":"10.1242\/bio.20147641","type":"journal-article","created":{"date-parts":[[2014,5,24]],"date-time":"2014-05-24T04:29:37Z","timestamp":1400905777000},"page":"494-502","source":"Crossref","is-referenced-by-count":41,"title":["Low dose tubulin-binding drugs rescue peroxisome trafficking deficit in patient-derived stem cells in Hereditary Spastic Paraplegia"],"prefix":"10.1242","volume":"3","author":[{"given":"Yongjun","family":"Fan","sequence":"first","affiliation":[{"name":"National Centre for Adult Stem Cell Research, Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gautam","family":"Wali","sequence":"additional","affiliation":[{"name":"National Centre for Adult Stem Cell Research, Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratneswary","family":"Sutharsan","sequence":"additional","affiliation":[{"name":"National Centre for Adult Stem Cell Research, Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernadette","family":"Bellette","sequence":"additional","affiliation":[{"name":"National Centre for Adult Stem Cell Research, Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis I.","family":"Crane","sequence":"additional","affiliation":[{"name":"National Centre for Adult Stem Cell Research, Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn M.","family":"Sue","sequence":"additional","affiliation":[{"name":"Kolling Institute for Medical Research, University of Sydney, Sydney, NSW 2065, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Mackay-Sim","sequence":"additional","affiliation":[{"name":"National Centre for Adult Stem Cell Research, Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2014,5,23]]},"reference":[{"key":"2021111216422534800_b1","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1242\/dmm.010884","article-title":"A patient-derived stem cell model of hereditary spastic paraplegia with SPAST mutations.","volume":"6","author":"Abrahamsen","year":"2013","journal-title":"Dis. 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