{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:28:20Z","timestamp":1790328500042,"version":"4.1.0"},"reference-count":53,"publisher":"The Company of Biologists","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,2,1]]},"abstract":"<jats:p>We have previously shown that the paired-box transcription factors Pax1 and Pax9 synergistically act in the proper formation of the vertebral column. Nevertheless, downstream events of the Pax1\/Pax9 action and their target genes remain to be elucidated. We show, by analyzing Pax1;Pax9 double mutant mice, that expression of Bapx1 in the sclerotome requires the presence of Pax1 and Pax9, in a gene dose-dependent manner. By using a retroviral system to overexpress Pax1 in chick presomitic mesoderm explants, we show that Pax1 can substitute for Shh in inducing Bapx1expression and in initiating chondrogenic differentiation. Furthermore, we demonstrate that Pax1 and Pax9 can transactivate regulatory sequences in theBapx1 promoter and that they physically interact with theBapx1 promoter region. These results strongly suggest thatBapx1 is a direct target of Pax1 and Pax9. Together, we conclude that Pax1 and Pax9 are required and sufficient for the chondrogenic differentiation of sclerotomal cells.<\/jats:p>","DOI":"10.1242\/dev.00240","type":"journal-article","created":{"date-parts":[[2003,1,6]],"date-time":"2003-01-06T23:31:09Z","timestamp":1041895869000},"page":"473-482","source":"Crossref","is-referenced-by-count":136,"title":["Pax1 and Pax9 activate<i>Bapx1<\/i>to induce chondrogenic differentiation in the sclerotome"],"prefix":"10.1242","volume":"130","author":[{"given":"Isabel","family":"Rodrigo","sequence":"first","affiliation":[{"name":"GSF-National Research Center for Environment and Health, Institute of Developmental Genetics, Ingolsta\u0308dter Landstrasse 1, 85764 Neuherberg,Germany"},{"name":"Present address: Instituto Cajal, CSIC, Dr Arce 37, 28002 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E.","family":"Hill","sequence":"additional","affiliation":[{"name":"MRC Human Genetics Unit, Western General Hospital, Crewe Rd, Edinburgh EH4 2XU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudi","family":"Balling","sequence":"additional","affiliation":[{"name":"German Research Centre for Biotechnology, Mascheroder Weg 1, 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Mu\u0308nsterberg","sequence":"additional","affiliation":[{"name":"University of East Anglia, School of Biological Sciences, Norwich NR4 7TJ,UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Imai","sequence":"additional","affiliation":[{"name":"GSF-National Research Center for Environment and Health, Institute of Developmental Genetics, Ingolsta\u0308dter Landstrasse 1, 85764 Neuherberg,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042604315994300_REF1","doi-asserted-by":"crossref","unstructured":"Adams, B., Dorfler, P., Aguzzi, A., Kozmik, Z., Urbanek, P.,Maurer-Fogy, I. and Busslinger, M. 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