{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:22:49Z","timestamp":1791274969168,"version":"4.1.0"},"reference-count":36,"publisher":"The Company of Biologists","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,5,1]]},"abstract":"<jats:p>Inversin (Inv), a protein that contains ankyrin repeats, plays a key role in left-right determination during mammalian embryonic development, but its precise function remains unknown. Transgenic mice expressing an Inv and green fluorescent protein (GFP) fusion construct (Inv::GFP) were established to facilitate characterization of the subcellular localization of Inv. TheInv::GFP transgene rescued the laterality defects and polycystic kidney disease of Inv\/Inv mice, indicating that the fusion protein is functional. In transgenic embryos, Inv::GFP protein was detected in the node monocilia. The fusion protein was also present in other 9+0 monocilia,including those of kidney epithelial cells and the pituitary gland, but it was not localized to 9+2 cilia. The N-terminal region of Inv (Inv\u0394C)including the ankyrin repeats also localized to the node cilia and rescued the left-right defects of Inv\/Inv mutants. Although no obvious abnormalities were detected in the node monocilia of Inv\/Inv embryos,the laterality defects of such embryos were corrected by an artificial leftward flow of fluid in the node, suggesting that nodal flow is impaired by the Inv mutation. These results suggest that the Inv protein contributes to left-right determination as a component of monocilia in the node and is essential for the generation of normal nodal flow.<\/jats:p>","DOI":"10.1242\/dev.00407","type":"journal-article","created":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T16:12:15Z","timestamp":1048003935000},"page":"1725-1734","source":"Crossref","is-referenced-by-count":177,"title":["The left-right determinant Inversin is a component of node monocilia and other 9+0 cilia"],"prefix":"10.1242","volume":"130","author":[{"given":"Daisuke","family":"Watanabe","sequence":"first","affiliation":[{"name":"Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan"},{"name":"CREST, Japan Science and Technology Corporation (JST)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukio","family":"Saijoh","sequence":"additional","affiliation":[{"name":"Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan"},{"name":"CREST, Japan Science and Technology Corporation (JST)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigenori","family":"Nonaka","sequence":"additional","affiliation":[{"name":"Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan"},{"name":"CREST, Japan Science and Technology Corporation (JST)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genta","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan"},{"name":"CREST, Japan Science and Technology Corporation (JST)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yayoi","family":"Ikawa","sequence":"additional","affiliation":[{"name":"Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan"},{"name":"CREST, Japan Science and Technology Corporation (JST)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiko","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology, Tokyo Women's Medical University, School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8600,Japan"},{"name":"Present address: Department of Anatomy and Developmental Biology, Kyoto Prefecture University of Medicine, Kawaramachi-Hirokoji, Kamikyo-ku, Kyoto 602-0841, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Hamada","sequence":"additional","affiliation":[{"name":"Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan"},{"name":"CREST, Japan Science and Technology Corporation (JST)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042612011302700_REF1","doi-asserted-by":"crossref","unstructured":"Akiyama, Y. and Ito, K. 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Cytol.203,357-381.","DOI":"10.1016\/S0074-7696(01)03011-X"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/130\/9\/1725\/1145476\/1725.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/130\/9\/1725\/1145476\/1725.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T13:55:51Z","timestamp":1704808551000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/130\/9\/1725\/52386\/The-left-right-determinant-Inversin-is-a-component"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5,1]]},"references-count":36,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2003,5,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.00407","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2003,5,1]]}}}