{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:51:59Z","timestamp":1792741919152,"version":"4.5.0"},"reference-count":50,"publisher":"The Company of Biologists","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,1,15]]},"abstract":"<jats:p>The trafficking of intracellular vesicles is essential for a number of cellular processes and defects in this process have been implicated in a wide range of human diseases. We identify the zebrafish mutant lbk as a novel model for such disorders. lbk displays hypopigmentation of skin melanocytes and the retinal pigment epithelium (RPE), an absence of iridophore reflections, defects in internal organs (liver, intestine) as well as functional defects in vision and the innate immune system (macrophages). Positional cloning, an allele screen, rescue experiments and morpholino knock-down reveal a mutation in the zebrafish orthologue of the vam6\/vps39 gene. Vam6p is part of the HOPS complex, which is essential for vesicle tethering and fusion. Affected cells in the lbkRPE, liver, intestine and macrophages display increased numbers and enlarged intracellular vesicles. Physiological and behavioural analyses reveal severe defects in visual ability in lbk mutants. The present study provides the first phenotypic description of a lack of vam6 gene function in a multicellular organism. lbk shares many of the characteristics of human diseases and suggests a novel disease gene for pathologies associated with defective vesicle transport, including the arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, the Hermansky-Pudlak syndrome, the Chediak-Higashi syndrome and the Griscelli syndrome.<\/jats:p>","DOI":"10.1242\/dev.006098","type":"journal-article","created":{"date-parts":[[2007,12,13]],"date-time":"2007-12-13T01:44:57Z","timestamp":1197510297000},"page":"387-399","source":"Crossref","is-referenced-by-count":47,"title":["The zebrafish mutant<i>lbk\/vam6<\/i>resembles human multisystemic disorders caused by aberrant trafficking of endosomal vesicles"],"prefix":"10.1242","volume":"135","author":[{"given":"Helia B.","family":"Schonthaler","sequence":"first","affiliation":[{"name":"Swiss Federal Institute of Technology, Department of Biology, and Brain Research Institute of the University of Zurich, Winterthurerstrasse 190,CH-8057 Zurich, Switzerland."},{"name":"Institute of Zoology, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie C.","family":"Fleisch","sequence":"additional","affiliation":[{"name":"Swiss Federal Institute of Technology, Department of Biology, and Brain Research Institute of the University of Zurich, Winterthurerstrasse 190,CH-8057 Zurich, Switzerland."},{"name":"Institute of Zoology, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Biehlmaier","sequence":"additional","affiliation":[{"name":"Institute of Zoology, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Makhankov","sequence":"additional","affiliation":[{"name":"Swiss Federal Institute of Technology, Department of Biology, and Brain Research Institute of the University of Zurich, Winterthurerstrasse 190,CH-8057 Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Rinner","sequence":"additional","affiliation":[{"name":"Swiss Federal Institute of Technology, Department of Biology, and Brain Research Institute of the University of Zurich, Winterthurerstrasse 190,CH-8057 Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronja","family":"Bahadori","sequence":"additional","affiliation":[{"name":"Swiss Federal Institute of Technology, Department of Biology, and Brain Research Institute of the University of Zurich, Winterthurerstrasse 190,CH-8057 Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Geisler","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Developmental Biology, Spemannstrasse 35, D-72076 Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinz","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Developmental Biology, Spemannstrasse 35, D-72076 Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan C. 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Commun.318,148-155.","DOI":"10.1016\/j.bbrc.2004.04.006"},{"key":"2021042605502329800_REF5","doi-asserted-by":"crossref","unstructured":"Brooks, D. A., Muller, V. J. and Hopwood, J. J.(2006). Stop-codon read-through for patients affected by a lysosomal storage disorder. Trends Mol. Med.12,367-373.","DOI":"10.1016\/j.molmed.2006.06.001"},{"key":"2021042605502329800_REF6","doi-asserted-by":"crossref","unstructured":"Caplan, S., Hartnell, L. M., Aguilar, R. C., Naslavsky, N. and Bonifacino, J. S. (2001). Human Vam6p promotes lysosome clustering and fusion in vivo. J. Cell Biol. 154,109-122.","DOI":"10.1083\/jcb.200102142"},{"key":"2021042605502329800_REF7","doi-asserted-by":"crossref","unstructured":"Clark, R. and Griffiths, G. M. (2003). Lytic granules, secretory lysosomes and disease. Curr. Opin. Immunol.15,516-521.","DOI":"10.1016\/S0952-7915(03)00113-4"},{"key":"2021042605502329800_REF8","doi-asserted-by":"crossref","unstructured":"Collins, K. M., Thorngren, N. L., Fratti, R. 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Mutations in VPS33B, encoding a regulator of SNARE-dependent membrane fusion, cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome. Nat. Genet.36,400-404.","DOI":"10.1038\/ng1325"},{"key":"2021042605502329800_REF18","doi-asserted-by":"crossref","unstructured":"Glass, A. S. and Dahm, R. (2004). The zebrafish as a model organism for eye development. Ophthalmic Res.36,4-24.","DOI":"10.1159\/000076105"},{"key":"2021042605502329800_REF19","doi-asserted-by":"crossref","unstructured":"Griscelli, C., Durandy, A., Guy-Grand, D., Daguillard, F.,Herzog, C. and Prunieras, M. (1978). A syndrome associating partial albinism and immunodeficiency. Am. J. Med.65,691-702.","DOI":"10.1016\/0002-9343(78)90858-6"},{"key":"2021042605502329800_REF20","doi-asserted-by":"crossref","unstructured":"Haffter, P., Granato, M., Brand, M., Mullins, M. C.,Hammerschmidt, M., Kane, D. A., Odenthal, J., van Eeden, F. J., Jiang, Y. J.,Heisenberg, C. P. et al. (1996). 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