{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:21:19Z","timestamp":1793254879977,"version":"4.5.0"},"reference-count":63,"publisher":"The Company of Biologists","issue":"25","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,12,22]]},"abstract":"<jats:p>Fibroblast growth factor 8 (Fgf8) is expressed in many domains of the developing embryo. Globally decreased FGF8 signaling during murine embryogenesis results in a hypomorphic phenotype with a constellation of heart, outflow tract, great vessel and pharyngeal gland defects that phenocopies human deletion 22q11 syndromes, such as DiGeorge. We postulate that these Fgf8 hypomorphic phenotypes result from disruption of local FGF8 signaling from pharyngeal arch epithelia to mesenchymal cells populating and migrating through the third and fourth pharyngeal arches.<\/jats:p>\n                  <jats:p>To test our hypothesis, and to determine whether the pharyngeal ectoderm and endoderm Fgf8 expression domains have discrete functional roles,we performed conditional mutagenesis of Fgf8 using novel Crerecombinase drivers to achieve domain-specific ablation of Fgf8gene function in the pharyngeal arch ectoderm and endoderm.<\/jats:p>\n                  <jats:p>Remarkably, ablating FGF8 protein in the pharyngeal arch ectoderm causes failure of formation of the fourth pharyngeal arch artery that results in aortic arch and subclavian artery anomalies in 95% of mutants; these defects recapitulate the spectrum and frequency of vascular defects reported in Fgf8 hypomorphs. Surprisingly, no cardiac, outflow tract or glandular defects were found in ectodermal-domain mutants, indicating that ectodermally derived FGF8 has essential roles during pharyngeal arch vascular development distinct from those in cardiac, outflow tract and pharyngeal gland morphogenesis. By contrast, ablation of FGF8 in the third and fourth pharyngeal endoderm and ectoderm caused glandular defects and bicuspid aortic valve, which indicates that the FGF8 endodermal domain has discrete roles in pharyngeal and valvar development. These results support our hypotheses that local FGF8 signaling from the pharyngeal epithelia is required for pharyngeal vascular and glandular development, and that the pharyngeal ectodermal and endodermal domains of FGF8 have separate functions.<\/jats:p>","DOI":"10.1242\/dev.00850","type":"journal-article","created":{"date-parts":[[2003,11,17]],"date-time":"2003-11-17T19:37:52Z","timestamp":1069097872000},"page":"6361-6374","source":"Crossref","is-referenced-by-count":220,"title":["Ablation of specific expression domains reveals discrete functions of ectoderm- and endoderm-derived FGF8 during cardiovascular and pharyngeal development"],"prefix":"10.1242","volume":"130","author":[{"given":"Timothy L.","family":"Macatee","sequence":"first","affiliation":[{"name":"Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin P.","family":"Hammond","sequence":"additional","affiliation":[{"name":"Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"},{"name":"Childrens Health Research Center, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin R.","family":"Arenkiel","sequence":"additional","affiliation":[{"name":"Program in Molecular Biology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily","family":"Francis","sequence":"additional","affiliation":[{"name":"Program in Neuroscience, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah U.","family":"Frank","sequence":"additional","affiliation":[{"name":"Childrens Health Research Center, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"},{"name":"Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne M.","family":"Moon","sequence":"additional","affiliation":[{"name":"Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"},{"name":"Childrens Health Research Center, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"},{"name":"Program in Molecular Biology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"},{"name":"Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"},{"name":"Department of Neurobiology and Anatomy, University of Utah School of Medicine,Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042604423583000_REF1","unstructured":"Abu-Issa, R., Smyth, G., Smoak, I., Yamamura, K. and Meyers, E. N. (2002). Fgf8 is required for pharyngeal arch and cardiovascular development in the mouse. Development129,4613-4625."},{"key":"2021042604423583000_REF2","doi-asserted-by":"crossref","unstructured":"Alsan, B. H. and Schultheiss, T. M. (2002). Regulation of avian cardiogenesis by Fgf8 signaling. Development129,1935-1943.","DOI":"10.1242\/dev.129.8.1935"},{"key":"2021042604423583000_REF3","doi-asserted-by":"crossref","unstructured":"Auerbach, R. (1960). Morphogenetic interactions in the development of hte mouse thymus gland. Dev. Biol.2,271-284.","DOI":"10.1016\/0012-1606(60)90009-9"},{"key":"2021042604423583000_REF4","doi-asserted-by":"crossref","unstructured":"Bockman, D. E. and Kirby, M. L. (1984). Dependence of thymus development on derivatives of the neural crest. Science223,498-500.","DOI":"10.1126\/science.6606851"},{"key":"2021042604423583000_REF5","doi-asserted-by":"crossref","unstructured":"Bockman, D. E., Redmond, M. E. and Kirby, M. L.(1989). Alteration of early vascular development after ablation of cranial neural crest. Anat. Rec.225,209-217.","DOI":"10.1002\/ar.1092250306"},{"key":"2021042604423583000_REF6","doi-asserted-by":"crossref","unstructured":"Brewer, S., Jiang, X., Donaldson, S., Williams, T. and Sucov, H. M. (2002). Requirement for AP-2alpha in cardiac outflow tract morphogenesis. Mech. Dev.110,139-149.","DOI":"10.1016\/S0925-4773(01)00579-2"},{"key":"2021042604423583000_REF7","doi-asserted-by":"crossref","unstructured":"Cleaver, O. and Krieg, P. A. (1999). Molecular mechanisms of vascular development. In Heart Development (ed. N. Rosenthal and R. P. Harvey), pp.221-252. San Diego: Academic Press.","DOI":"10.1016\/B978-012329860-7\/50016-7"},{"key":"2021042604423583000_REF8","doi-asserted-by":"crossref","unstructured":"Conway, S. J., Godt, R. E., Hatcher, C. J., Leatherbury, L.,Zolotouchnikov, V. V., Brotto, M. A., Copp, A. J., Kirby, M. L. and Creazzo,T. L. (1997a). Neural crest is involved in development of abnormal myocardial function. J. Mol. Cell. Cardiol.29,2675-2685.","DOI":"10.1006\/jmcc.1997.0499"},{"key":"2021042604423583000_REF9","doi-asserted-by":"crossref","unstructured":"Conway, S. J., Henderson, D. J. and Copp, A. J.(1997b). Pax3 is required for cardiac neural crest migration in the mouse: evidence from the splotch (Sp2H) mutant. Development124,505-514.","DOI":"10.1242\/dev.124.2.505"},{"key":"2021042604423583000_REF10","doi-asserted-by":"crossref","unstructured":"Conway, S. J., Henderson, D. J., Kirby, M. L., Anderson, R. H. and Copp, A. J. (1997c). Development of a lethal congenital heart defect in the splotch (Pax3) mutant mouse. Cardiovasc. Res.36,163-173.","DOI":"10.1016\/S0008-6363(97)00172-7"},{"key":"2021042604423583000_REF11","doi-asserted-by":"crossref","unstructured":"Crossley, P. H. and Martin, G. R. (1995). The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Development121,439-451.","DOI":"10.1242\/dev.121.2.439"},{"key":"2021042604423583000_REF12","doi-asserted-by":"crossref","unstructured":"de la Pompa, J. L., Timmerman, L. A., Takimoto, H., Yoshida, H.,Elia, A. J., Samper, E., Potter, J., Wakeham, A., Marengere, L., Langille, B. L. et al. (1998). Role of the NF-ATc transcription factor in morphogenesis of cardiac valves and septum. Nature392,182-186.","DOI":"10.1038\/32419"},{"key":"2021042604423583000_REF13","doi-asserted-by":"crossref","unstructured":"Dymecki, S. M. (1996). Flp recombinase promotes site-specific recombination in embryonic stem cells and transgenic mice. Proc. Natl. Acad. Sci. USA93,6191-6196.","DOI":"10.1073\/pnas.93.12.6191"},{"key":"2021042604423583000_REF14","doi-asserted-by":"crossref","unstructured":"Epstein, J. A. (2001). Developing models of DiGeorge syndrome. Trends Genet.17,S13-S17.","DOI":"10.1016\/S0168-9525(01)02450-7"},{"key":"2021042604423583000_REF15","doi-asserted-by":"crossref","unstructured":"Epstein, J. A., Li, J., Lang, D., Chen, F., Brown, C. B., Jin,F., Lu, M. M., Thomas, M., Liu, E., Wessels, A. et al.(2000). Migration of cardiac neural crest cells in Splotch embryos. Development127,1869-1878.","DOI":"10.1242\/dev.127.9.1869"},{"key":"2021042604423583000_REF16","unstructured":"Frank, D. U., Fotheringham, L. K., Brewer, J. A., Muglia, L. J.,Tristani-Firouzi, M., Capecchi, M. R. and Moon, A. M. (2002). An Fgf8 mouse mutant phenocopies human 22q11 deletion syndrome. Development129,4591-4603."},{"key":"2021042604423583000_REF17","doi-asserted-by":"crossref","unstructured":"Franz, T. (1989). Persistent truncus arteriosus in the Splotch mutant mouse. Anat. Embryol.180,457-464.","DOI":"10.1007\/BF00305120"},{"key":"2021042604423583000_REF18","doi-asserted-by":"crossref","unstructured":"Garel, S., Huffman, K. J. and Rubenstein, J. L.(2003). Molecular regionalization of the neocortex is disrupted in Fgf8 hypomorphic mutants. Development130,1903-1914.","DOI":"10.1242\/dev.00416"},{"key":"2021042604423583000_REF19","doi-asserted-by":"crossref","unstructured":"Goulding, M., Sterrer, S., Fleming, J., Balling, R., Nadeau, J.,Moore, K. J., Brown, S. D., Steel, K. P. and Gruss, P.(1993). Analysis of the Pax-3 gene in the mouse mutant splotch. Genomics17,355-363.","DOI":"10.1006\/geno.1993.1332"},{"key":"2021042604423583000_REF20","doi-asserted-by":"crossref","unstructured":"Graham, A. (2001). The development and evolution of the pharyngeal arches. J. Anat.199,133-141.","DOI":"10.1046\/j.1469-7580.2001.19910133.x"},{"key":"2021042604423583000_REF21","doi-asserted-by":"crossref","unstructured":"Graham, A. and Smith, A. (2001). Patterning the pharyngeal arches. BioEssays23, 54-61.","DOI":"10.1002\/1521-1878(200101)23:1<54::AID-BIES1007>3.0.CO;2-5"},{"key":"2021042604423583000_REF22","doi-asserted-by":"crossref","unstructured":"Hood, L. C. and Rosenquist, T. H. (1992). Coronary artery development in the chick: origin and deployment of smooth muscle cells, and the effects of neural crest ablation. Anat. Rec.234,291-300.","DOI":"10.1002\/ar.1092340215"},{"key":"2021042604423583000_REF23","doi-asserted-by":"crossref","unstructured":"Jackson, R. J., Howell, M. T. and Kaminski, A.(1990). The novel mechanism of initiation of picornavirus RNA translation. Trends Biochem Sci.15,477-483.","DOI":"10.1016\/0968-0004(90)90302-R"},{"key":"2021042604423583000_REF24","doi-asserted-by":"crossref","unstructured":"Jang, S. K. and Wimmer, E. (1990). Cap-independent translation of encephalomyocarditis virus RNA: structural elements of the internal ribosomal entry site and involvement of a cellular 57-kD RNA-binding protein. Genes Dev.4,1560-1572.","DOI":"10.1101\/gad.4.9.1560"},{"key":"2021042604423583000_REF25","doi-asserted-by":"crossref","unstructured":"Jerome, L. A. and Papaioannou, V. E. (2001). DiGeorge syndrome phenotype in mice mutant for the T-box gene, Tbx1. Nat. Genet.27,286-291.","DOI":"10.1038\/85845"},{"key":"2021042604423583000_REF26","doi-asserted-by":"crossref","unstructured":"Jiang, X., Rowitch, D. H., Soriano, P., McMahon, A. P. and Sucov, H. M. (2000). Fate of the mammalian cardiac neural crest. Development127,1607-1616.","DOI":"10.1242\/dev.127.8.1607"},{"key":"2021042604423583000_REF27","doi-asserted-by":"crossref","unstructured":"Kirby, M. L. and Waldo, K. L. (1990). Role of neural crest in congenital heart disease. Circulation82,332-340.","DOI":"10.1161\/01.CIR.82.2.332"},{"key":"2021042604423583000_REF28","doi-asserted-by":"crossref","unstructured":"Kirby, M. L. and Waldo, K. L. (1995). Neural crest and cardiovascular patterning. Circ. Res.77,211-215.","DOI":"10.1161\/01.RES.77.2.211"},{"key":"2021042604423583000_REF29","doi-asserted-by":"crossref","unstructured":"Kirby, M. L., Turnage, K. L., 3rd and Hays, B. M.(1985). Characterization of conotruncal malformations following ablation of `cardiac' neural crest. Anat. Rec.213, 87-93.","DOI":"10.1002\/ar.1092130112"},{"key":"2021042604423583000_REF30","doi-asserted-by":"crossref","unstructured":"Kirby, M. L., Hunt, P., Wallis, K. and Thorogood, P.(1997). Abnormal patterning of the aortic arch arteries does not evoke cardiac malformations. Dev. Dyn.208, 34-47.","DOI":"10.1002\/(SICI)1097-0177(199701)208:1<34::AID-AJA4>3.0.CO;2-2"},{"key":"2021042604423583000_REF31","doi-asserted-by":"crossref","unstructured":"Kwee, L., Baldwin, H. S., Shen, H. M., Stewart, C. L., Buck, C.,Buck, C. A. and Labow, M. A. (1995). Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice. Development121,489-503.","DOI":"10.1242\/dev.121.2.489"},{"key":"2021042604423583000_REF32","unstructured":"LeDouarin, N. and Jotereau, F. V. (1975). Tracing of cells of the avian thymus through embryonic life in interspecific chimeras. J. Exp. Med.142."},{"key":"2021042604423583000_REF33","doi-asserted-by":"crossref","unstructured":"Lee, T. C., Zhao, Y. D., Courtman, D. W. and Stewart, D. J.(2000). Abnormal aortic valve development in mice lacking endothelial nitric oxide synthase. Circulation101,2345-2348.","DOI":"10.1161\/01.CIR.101.20.2345"},{"key":"2021042604423583000_REF34","unstructured":"LeLievre, C. S. and LeDouarin, N. (1975). Mesenchymal derivatives of the neural crest: analysis of chimaeric quail and chick embryos. J. Embryol. Exp. Morphol.34,125-154."},{"key":"2021042604423583000_REF35","doi-asserted-by":"crossref","unstructured":"Li, J., Chen, F. and Epstein, J. A. (2000). Neural crest expression of Cre recombinase directed by the proximal Pax3 promoter in transgenic mice. Genesis26,162-164.","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<162::AID-GENE21>3.0.CO;2-R"},{"key":"2021042604423583000_REF36","doi-asserted-by":"crossref","unstructured":"Li, W. E., Waldo, K., Linask, K. L., Chen, T., Wessels, A.,Parmacek, M. S., Kirby, M. L. and Lo, C. W. (2002). An essential role for connexin43 gap junctions in mouse coronary artery development. Development129,2031-2042.","DOI":"10.1242\/dev.129.8.2031"},{"key":"2021042604423583000_REF37","doi-asserted-by":"crossref","unstructured":"Lindsay, E. (2001). Chromosomal microdeletions:dissecting del22q11 syndrome. Nat. Rev. Gen2, 858-868.","DOI":"10.1038\/35098574"},{"key":"2021042604423583000_REF38","doi-asserted-by":"crossref","unstructured":"Lindsay, E. A., Vitelli, F., Su, H., Morishima, M., Huynh, T.,Pramparo, T., Jurecic, V., Ogunrinu, G., Sutherland, H. F., Scambler, P. J. et al. (2001). Tbx1 haploinsufficiency in the DiGeorge syndrome region causes aortic arch defects in mice. Nature410,97-101.","DOI":"10.1038\/35065105"},{"key":"2021042604423583000_REF39","doi-asserted-by":"crossref","unstructured":"MacArthur, C. A., Lawshe, A., Xu, J., Santos-Ocampo, S.,Heikinheimo, M., Chellaiah, A. T. and Ornitz, D. M. (1995). FGF-8 isoforms activate receptor splice forms that are expressed in mesenchymal regions of mouse development. Development121,3603-3613.","DOI":"10.1242\/dev.121.11.3603"},{"key":"2021042604423583000_REF40","doi-asserted-by":"crossref","unstructured":"Manley, N. R. and Capecchi, M. R. (1995). The role of Hoxa-3 in mouse thymus and thyroid development. Development121,1989-2003.","DOI":"10.1242\/dev.121.7.1989"},{"key":"2021042604423583000_REF41","doi-asserted-by":"crossref","unstructured":"Merscher, S., Funke, B., Epstein, J. A., Heyer, J., Puech, A.,Lu, M. M., Xavier, R. J., Demay, M. B., Russell, R. G., Factor, S. et al.(2001). TBX1 is responsible for cardiovascular defects in velo-cardio-facial\/DiGeorge syndrome. Cell104,619-629.","DOI":"10.1016\/S0092-8674(01)00247-1"},{"key":"2021042604423583000_REF42","doi-asserted-by":"crossref","unstructured":"Meyers, E. N. and Martin, G. R. (1999). Differences in left-right axis pathways in mouse and chick: functions of FGF8 and SHH. Science285,403-406.","DOI":"10.1126\/science.285.5426.403"},{"key":"2021042604423583000_REF43","doi-asserted-by":"crossref","unstructured":"Meyers, E. N., Lewandoski, M. and Martin, G. R.(1998). An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Nat. Genet.18,136-141.","DOI":"10.1038\/ng0298-136"},{"key":"2021042604423583000_REF44","doi-asserted-by":"crossref","unstructured":"Mikawa, T. and Gourdie, R. G. (1996). Pericardial mesoderm generates a population of coronary smooth muscle cells migrating into the heart along with ingrowth of the epicardial organ. Dev. Biol.174,221-232.","DOI":"10.1006\/dbio.1996.0068"},{"key":"2021042604423583000_REF45","doi-asserted-by":"crossref","unstructured":"Mitchell, P. J., Timmons, P. M., Hebert, J. M., Rigby, P. W. and Tjian, R. (1991). Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis. Genes Dev.5,105-119.","DOI":"10.1101\/gad.5.1.105"},{"key":"2021042604423583000_REF46","doi-asserted-by":"crossref","unstructured":"Moon, A. M., Boulet, A. M. and Capecchi, M. R.(2000). Normal limb development in conditional mutants of Fgf4. Development127,989-996.","DOI":"10.1242\/dev.127.5.989"},{"key":"2021042604423583000_REF47","doi-asserted-by":"crossref","unstructured":"Moon, A. M. and Capecchi, M. R. (2000). FGF8 is required for outgrowth and patterning of the limbs. Nat. Genet.26,455-459.","DOI":"10.1038\/82601"},{"key":"2021042604423583000_REF48","doi-asserted-by":"crossref","unstructured":"Ranger, A. M., Grusby, M. J., Hodge, M. R., Gravallese, E. M.,de la Brousse, F. C., Hoey, T., Mickanin, C., Baldwin, H. S. and Glimcher, L. H. (1998). The transcription factor NF-ATc is essential for cardiac valve formation. Nature392,186-190.","DOI":"10.1038\/32426"},{"key":"2021042604423583000_REF49","doi-asserted-by":"crossref","unstructured":"Reifers, F., Walsh, E. C., Leger, S., Stainier, D. Y. and Brand,M. (2000). Induction and differentiation of the zebrafish heart requires fibroblast growth factor 8 (fgf8\/acerebellar). Development127,225-235.","DOI":"10.1242\/dev.127.2.225"},{"key":"2021042604423583000_REF50","doi-asserted-by":"crossref","unstructured":"Sauer, B. and Henderson, N. (1988). Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc. Natl. Acad. Sci. USA85,5166-5170.","DOI":"10.1073\/pnas.85.14.5166"},{"key":"2021042604423583000_REF51","doi-asserted-by":"crossref","unstructured":"Scambler, P. J. (2000). The 22q11 deletion syndromes. Hum. Mol. Genet.9,2421-2426.","DOI":"10.1093\/hmg\/9.16.2421"},{"key":"2021042604423583000_REF52","doi-asserted-by":"crossref","unstructured":"Schwenk, F., Baron, U. and Rajewsky, K. (1995). A cre-transgenic mouse strain for the ubiquitous deletion of loxP-flanked gene segments including deletion in germ cells. Nucleic Acids Res.23,5080-5081.","DOI":"10.1093\/nar\/23.24.5080"},{"key":"2021042604423583000_REF53","doi-asserted-by":"crossref","unstructured":"Soriano, P. (1999). Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat. Genet.21, 70-71.","DOI":"10.1038\/5007"},{"key":"2021042604423583000_REF54","unstructured":"Stadler, H. S., Higgins, K. M. and Capecchi, M. R.(2001). Loss of Eph-receptor expression correlates with loss of cell adhesion and chondrogenic capacity in Hoxa13 mutant limbs. Development128,4177-4188."},{"key":"2021042604423583000_REF55","doi-asserted-by":"crossref","unstructured":"Storm, E. E., Rubenstein, J. L. and Martin, G. R.(2003). Dosage of Fgf8 determines whether cell survival is positively or negatively regulated in the developing forebrain. Proc. Natl. Acad. Sci. USA100,1757-1762.","DOI":"10.1073\/pnas.0337736100"},{"key":"2021042604423583000_REF56","doi-asserted-by":"crossref","unstructured":"Sun, X., Meyers, E. N., Lewandoski, M. and Martin, G. R.(1999). Targeted disruption of Fgf8 causes failure of cell migration in the gastrulating mouse embryo. Genes Dev.13,1834-1846.","DOI":"10.1101\/gad.13.14.1834"},{"key":"2021042604423583000_REF57","doi-asserted-by":"crossref","unstructured":"Sun, X., Lewandoski, M., Meyers, E. N., Liu, Y. H., Maxson, R. E., Jr and Martin, G. R. (2000). Conditional inactivation of Fgf4 reveals complexity of signalling during limb bud development. Nat. Genet.25,83-86.","DOI":"10.1038\/75644"},{"key":"2021042604423583000_REF58","doi-asserted-by":"crossref","unstructured":"Trumpp, A., Depew, M. J., Rubenstein, J. L., Bishop, J. M. and Martin, G. R. (1999). Cre-mediated gene inactivation demonstrates that FGF8 is required for cell survival and patterning of the first branchial arch. Genes Dev13,3136-3148.","DOI":"10.1101\/gad.13.23.3136"},{"key":"2021042604423583000_REF59","unstructured":"Vitelli, F., Taddei, I., Morishima, M., Meyers, E. N., Lindsay,E. A. and Baldini, A. (2002). A genetic link between Tbx1 and fibroblast growth factor signaling. Development129,4605-4611."},{"key":"2021042604423583000_REF60","doi-asserted-by":"crossref","unstructured":"Waldo, K. L., Kumiski, D. H. and Kirby, M. L.(1994). Association of the cardiac neural crest with development of the coronary arteries in the chick embryo. Anat. Rec.239,315-331.","DOI":"10.1002\/ar.1092390310"},{"key":"2021042604423583000_REF61","doi-asserted-by":"crossref","unstructured":"Ya, J., Schilham, M. W., de Boer, P. A., Moorman, A. F.,Clevers, H. and Lamers, W. H. (1998). Sox4-deficiency syndrome in mice is an animal model for common trunk. Circ. Res.83,986-994.","DOI":"10.1161\/01.RES.83.10.986"},{"key":"2021042604423583000_REF62","doi-asserted-by":"crossref","unstructured":"Yamagishi, H., Maeda, J., Hu, T., McAnally, J., Conway, S. J.,Kume, T., Meyers, E. N., Yamagishi, C. and Srivastava, D.(2003). Tbx1 is regulated by tissue-specific forkhead proteins through a common Sonic hedgehog-responsive enhancer. Genes Dev.17,269-281.","DOI":"10.1101\/gad.1048903"},{"key":"2021042604423583000_REF63","doi-asserted-by":"crossref","unstructured":"Yang, J. T., Rando, T. A., Mohler, W. A., Rayburn, H., Blau, H. M. and Hynes, R. O. (1996). Genetic analysis of alpha 4 integrin functions in the development of mouse skeletal muscle. J. 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