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These progenitors were also capable of colonising wild-type and aganglionic gut in organ culture and had the potential to generate differentiated progeny that localised within the intrinsic ganglionic plexus. Similar progenitors were also derived from the normoganglionic small intestine of mice with colonic aganglionosis. Our findings establish the feasibility of expanding and isolating early progenitors of the enteric nervous system based on their ability to form distinct neurogenic and gliogenic structures in culture. Furthermore, these experiments provide the rationale for the development of novel approaches to the treatment of congenital megacolon (Hirschsprung's disease) based on the colonisation of the aganglionic gut with progenitors derived from normoganglionic bowel segments.<\/jats:p>","DOI":"10.1242\/dev.00857","type":"journal-article","created":{"date-parts":[[2003,11,17]],"date-time":"2003-11-17T19:37:52Z","timestamp":1069097872000},"page":"6387-6400","source":"Crossref","is-referenced-by-count":205,"title":["Neuron and glia generating progenitors of the mammalian enteric nervous system isolated from foetal and postnatal gut cultures"],"prefix":"10.1242","volume":"130","author":[{"given":"Nadege","family":"Bondurand","sequence":"first","affiliation":[{"name":"Division of Molecular Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipa","family":"Natarajan","sequence":"additional","affiliation":[{"name":"Division of Molecular Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Thapar","sequence":"additional","affiliation":[{"name":"Division of Molecular Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Atkins","sequence":"additional","affiliation":[{"name":"Division of Immunoregulation, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vassilis","family":"Pachnis","sequence":"additional","affiliation":[{"name":"Division of Molecular Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042604424610400_REF1","doi-asserted-by":"crossref","unstructured":"Baetge, G. and Gershon, M. 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