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These analyses implicate new signaling pathways in endocrine pancreas development, and identified sets of known and novel genes that are temporally regulated, as well as genes that spatially define developing endocrine cells from their neighbors. The differential expression of several genes from each time point was verified by RT-PCR and in situ hybridization. Moreover, we present preliminary functional evidence suggesting that one transcription factor encoding gene (Myt1), which was identified in our screen, is expressed in endocrine progenitors and may regulate \u03b1, \u03b2 and\u03b4 cell development. In addition to identifying new genes that regulate endocrine cell fate, this global gene expression analysis has uncovered informative biological trends that occur during endocrine differentiation.<\/jats:p>","DOI":"10.1242\/dev.00921","type":"journal-article","created":{"date-parts":[[2003,12,9]],"date-time":"2003-12-09T01:12:49Z","timestamp":1070932369000},"page":"165-179","source":"Crossref","is-referenced-by-count":200,"title":["Global expression analysis of gene regulatory pathways during endocrine pancreatic development"],"prefix":"10.1242","volume":"131","author":[{"given":"Guoqiang","family":"Gu","sequence":"first","affiliation":[{"name":"1Department of Molecular and Cellular Biology, Harvard University, Cambridge,MA 02138, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Wells","sequence":"additional","affiliation":[{"name":"1Department of Molecular and Cellular Biology, Harvard University, Cambridge,MA 02138, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Dombkowski","sequence":"additional","affiliation":[{"name":"2Department of Pathology, Massachusetts General Hospital, Charlestown,Massachusetts 02129, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred","family":"Preffer","sequence":"additional","affiliation":[{"name":"2Department of Pathology, Massachusetts General Hospital, Charlestown,Massachusetts 02129, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce","family":"Aronow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas A.","family":"Melton","sequence":"additional","affiliation":[{"name":"1Department of Molecular and Cellular Biology, Harvard University, Cambridge,MA 02138, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2024022007552048400_REF1","doi-asserted-by":"crossref","unstructured":"Abraham, E. 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