{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:35:00Z","timestamp":1790667300574,"version":"4.1.0"},"reference-count":52,"publisher":"The Company of Biologists","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,4,1]]},"abstract":"<jats:p>PTX3 is a prototypic long pentraxin that plays a non-redundant role in innate immunity against selected pathogens and in female fertility. Here, we report that the infertility of Ptx3\u2013\/\u2013 mice is associated with severe abnormalities of the cumulus oophorus and failure of in vivo, but not in vitro, oocyte fertilization. PTX3 is produced by mouse cumulus cells during cumulus expansion and localizes in the matrix. PTX3 is expressed in the human cumulus oophorus as well. Cumuli from Ptx3\u2013\/\u2013 mice synthesize normal amounts of hyaluronan (HA), but are unable to organize it in a stable matrix. Exogenous PTX3 restores a normal cumulus phenotype. Incorporation in the matrix of inter-\u03b1-trypsin inhibitor is normal in Ptx3\u2013\/\u2013 cumuli. PTX3 does not interact directly with HA, but it binds the cumulus matrix hyaladherin tumor necrosis factor \u03b1-induced protein 6 (TNFAIP6, also known as TSG6) and thereby may form multimolecular complexes that can cross-link HA chains. Thus, PTX3 is a structural constituent of the cumulus oophorus extracellular matrix essential for female fertility.<\/jats:p>","DOI":"10.1242\/dev.01056","type":"journal-article","created":{"date-parts":[[2004,3,9]],"date-time":"2004-03-09T01:23:43Z","timestamp":1078795423000},"page":"1577-1586","source":"Crossref","is-referenced-by-count":402,"title":["PTX3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilization"],"prefix":"10.1242","volume":"131","author":[{"given":"Antonietta","family":"Salustri","sequence":"first","affiliation":[{"name":"Department of Public Health and Cell Biology, University of Rome Tor Vergata,Rome 00133, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Garlanda","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology, Mario Negri Institute for Pharmacological Research, Milan 20157, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio","family":"Hirsch","sequence":"additional","affiliation":[{"name":"Department of Genetics, Biology and Biochemistry, University of Turin, Turin 10126, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marika","family":"De Acetis","sequence":"additional","affiliation":[{"name":"Department of Genetics, Biology and Biochemistry, University of Turin, Turin 10126, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Maccagno","sequence":"additional","affiliation":[{"name":"Department of Public Health and Cell Biology, University of Rome Tor Vergata,Rome 00133, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Bottazzi","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology, Mario Negri Institute for Pharmacological Research, Milan 20157, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Doni","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology, Mario Negri Institute for Pharmacological Research, Milan 20157, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Bastone","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology, Mario Negri Institute for Pharmacological Research, Milan 20157, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Mantovani","sequence":"additional","affiliation":[{"name":"Institute of Endocrinology, Universita\u0300 degli Studi di Milano, Milan 20100, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo Beck","family":"Peccoz","sequence":"additional","affiliation":[{"name":"Institute of Endocrinology, Universita\u0300 degli Studi di Milano, Milan 20100, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Salvatori","sequence":"additional","affiliation":[{"name":"SigmaTau SpA, Pomezia, Rome 00040, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Mahoney","sequence":"additional","affiliation":[{"name":"MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford,Oxford OX1 3QU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony J.","family":"Day","sequence":"additional","affiliation":[{"name":"MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford,Oxford OX1 3QU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregorio","family":"Siracusa","sequence":"additional","affiliation":[{"name":"Department of Public Health and Cell Biology, University of Rome Tor Vergata,Rome 00133, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigina","family":"Romani","sequence":"additional","affiliation":[{"name":"Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia 05122, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Mantovani","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology, Mario Negri Institute for Pharmacological Research, Milan 20157, Italy"},{"name":"Centro IDET, Institute of General Pathology, Universita\u0300 degli Studi di Milano, Milan 20100, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042605092027900_REF1","doi-asserted-by":"crossref","unstructured":"Biffo, S. and Tolosano, E. 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