{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:44:45Z","timestamp":1791139485308,"version":"4.1.0"},"reference-count":55,"publisher":"The Company of Biologists","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,5,1]]},"abstract":"<jats:p>Understanding the molecular mechanisms of stem cell maintenance is crucial for the ultimate goal of manipulating stem cells for the treatment of disease. Foxd3 is required early in mouse embryogenesis; Foxd3-\/-embryos fail around the time of implantation, cells of the inner cell mass cannot be maintained in vitro, and blastocyst-derived stem cell lines cannot be established. Here, we report that Foxd3 is required for maintenance of the multipotent mammalian neural crest. Using tissue-specific deletion of Foxd3 in the neural crest, we show that Foxd3flox\/-; Wnt1-Cre mice die perinatally with a catastrophic loss of neural crest-derived structures. Cranial neural crest tissues are either missing or severely reduced in size, the peripheral nervous system consists of reduced dorsal root ganglia and cranial nerves, and the entire gastrointestinal tract is devoid of neural crest derivatives. These results demonstrate a global role for this transcriptional repressor in all aspects of neural crest maintenance along the anterior-posterior axis, and establish an unprecedented molecular link between multiple divergent progenitor lineages of the mammalian embryo.<\/jats:p>","DOI":"10.1242\/dev.012179","type":"journal-article","created":{"date-parts":[[2008,3,27]],"date-time":"2008-03-27T01:00:07Z","timestamp":1206579607000},"page":"1615-1624","source":"Crossref","is-referenced-by-count":185,"title":["Requirement for Foxd3 in the maintenance of neural crest progenitors"],"prefix":"10.1242","volume":"135","author":[{"given":"Lu","family":"Teng","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan A.","family":"Mundell","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University School of Medicine,Nashville, TN 37232-0494, USA."},{"name":"Center for Stem Cell Biology, Vanderbilt University School of Medicine,Nashville, TN 37232-0494, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey Y.","family":"Frist","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0494, USA."},{"name":"Center for Stem Cell Biology, Vanderbilt University School of Medicine,Nashville, TN 37232-0494, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaohong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Labosky","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA."},{"name":"Department of Pharmacology, Vanderbilt University School of Medicine,Nashville, TN 37232-0494, USA."},{"name":"Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0494, USA."},{"name":"Center for Stem Cell Biology, Vanderbilt University School of Medicine,Nashville, TN 37232-0494, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042621221865300_REF1","doi-asserted-by":"crossref","unstructured":"Acampora, D., Merlo, G. R., Paleari, L., Zerega, B.,Postiglione, M. P., Mantero, S., Bober, E., Barbieri, O., Simeone, A. and Levi, G. (1999). Craniofacial, vestibular and bone defects in mice lacking the Distal-less-related gene Dlx5. Development126,3795-3809.","DOI":"10.1242\/dev.126.17.3795"},{"key":"2021042621221865300_REF2","doi-asserted-by":"crossref","unstructured":"Avilion, A. A., Nicolis, S. K., Pevny, L. H., Perez, L., Vivian,N. and Lovell-Badge, R. (2003). Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev.17,126-140.","DOI":"10.1101\/gad.224503"},{"key":"2021042621221865300_REF3","doi-asserted-by":"crossref","unstructured":"Baynash, A. G., Hosoda, K., Giaid, A., Richardson, J. A., Emoto,N., Hammer, R. E. and Yanagisawa, M. (1994). Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons. Cell79,1277-1285.","DOI":"10.1016\/0092-8674(94)90018-3"},{"key":"2021042621221865300_REF4","doi-asserted-by":"crossref","unstructured":"Bellmeyer, A., Krase, J., Lindgren, J. and LaBonne, C.(2003). The protooncogene c-myc is an essential regulator of neural crest formation in Xenopus. Dev. Cell4, 827-839.","DOI":"10.1016\/S1534-5807(03)00160-6"},{"key":"2021042621221865300_REF5","doi-asserted-by":"crossref","unstructured":"Cacalano, G., Farinas, I., Wang, L. C., Hagler, K., Forgie, A.,Moore, M., Armanini, M., Phillips, H., Ryan, A. M., Reichardt, L. F. et al. (1998). GFRalpha1 is an essential receptor component for GDNF in the developing nervous system and kidney. Neuron21,53-62.","DOI":"10.1016\/S0896-6273(00)80514-0"},{"key":"2021042621221865300_REF6","doi-asserted-by":"crossref","unstructured":"Cheung, M. and Briscoe, J. (2003). Neural crest development is regulated by the transcription factor Sox9. Development130,5681-5693.","DOI":"10.1242\/dev.00808"},{"key":"2021042621221865300_REF7","doi-asserted-by":"crossref","unstructured":"Cheung, M., Chaboissier, M. C., Mynett, A., Hirst, E., Schedl,A. and Briscoe, J. (2005). The transcriptional control of trunk neural crest induction, survival, and delamination. Dev. Cell8,179-192.","DOI":"10.1016\/j.devcel.2004.12.010"},{"key":"2021042621221865300_REF8","doi-asserted-by":"crossref","unstructured":"Cirillo, L. A., Lin, F. R., Cuesta, I., Friedman, D., Jarnik, M. and Zaret, K. S. (2002). Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4. Mol. Cell9,279-289.","DOI":"10.1016\/S1097-2765(02)00459-8"},{"key":"2021042621221865300_REF9","doi-asserted-by":"crossref","unstructured":"Danielian, P. S., Muccino, D., Rowitch, D. H., Michael, S. K. and McMahon, A. P. (1998). Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase. Curr. Biol.8,1323-1326.","DOI":"10.1016\/S0960-9822(07)00562-3"},{"key":"2021042621221865300_REF10","doi-asserted-by":"crossref","unstructured":"De Bellard, M. E., Rao, Y. and Bronner-Fraser, M.(2003). Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells. J. Cell Biol.162,269-279.","DOI":"10.1083\/jcb.200301041"},{"key":"2021042621221865300_REF11","doi-asserted-by":"crossref","unstructured":"Ding, H., Wu, X., Kim, I., Tam, P. P., Koh, G. Y. and Nagy,A. (2000). The mouse Pdgfc gene: dynamic expression in embryonic tissues during organogenesis. Mech. Dev.96,209-213.","DOI":"10.1016\/S0925-4773(00)00425-1"},{"key":"2021042621221865300_REF12","doi-asserted-by":"crossref","unstructured":"Dolle, P., Price, M. and Duboule, D. (1992). Expression of the murine Dlx-1 homeobox gene during facial, ocular and limb development. Differentiation49, 93-99.","DOI":"10.1111\/j.1432-0436.1992.tb00773.x"},{"key":"2021042621221865300_REF13","doi-asserted-by":"crossref","unstructured":"Dottori, M., Gross, M. K., Labosky, P. and Goulding, M.(2001). The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate. Development128,4127-4138.","DOI":"10.1242\/dev.128.21.4127"},{"key":"2021042621221865300_REF14","doi-asserted-by":"crossref","unstructured":"Epstein, D. J., Vekemans, M. and Gros, P.(1991). Splotch (Sp2H), a mutation affecting development of the mouse neural tube, shows a deletion within the paired homeodomain of Pax-3. Cell67,767-774.","DOI":"10.1016\/0092-8674(91)90071-6"},{"key":"2021042621221865300_REF15","doi-asserted-by":"crossref","unstructured":"Feiner, L., Webber, A. L., Brown, C. B., Lu, M. M., Jia, L.,Feinstein, P., Mombaerts, P., Epstein, J. A. and Raper, J. A.(2001). Targeted disruption of semaphorin 3C leads to persistent truncus arteriosus and aortic arch interruption. Development128,3061-3070.","DOI":"10.1242\/dev.128.16.3061"},{"key":"2021042621221865300_REF16","doi-asserted-by":"crossref","unstructured":"Galan-Caridad, J. M., Harel, S., Arenzana, T. L., Hou, Z. E.,Doetsch, F. K., Mirny, L. A. and Reizis, B. (2007). Zfx controls the self-renewal of embryonic and hematopoietic stem cells. Cell129,345-357.","DOI":"10.1016\/j.cell.2007.03.014"},{"key":"2021042621221865300_REF17","doi-asserted-by":"crossref","unstructured":"Graham, V., Khudyakov, J., Ellis, P. and Pevny, L.(2003). SOX2 functions to maintain neural progenitor identity. Neuron39,749-765.","DOI":"10.1016\/S0896-6273(03)00497-5"},{"key":"2021042621221865300_REF18","doi-asserted-by":"crossref","unstructured":"Hanna, L. A., Foreman, R. K., Tarasenko, I. A., Kessler, D. S. and Labosky, P. A. (2002). Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryo. Genes Dev.16,2650-2661.","DOI":"10.1101\/gad.1020502"},{"key":"2021042621221865300_REF19","doi-asserted-by":"crossref","unstructured":"Hiruma, T., Nakajima, Y. and Nakamura, H.(2002). Development of pharyngeal arch arteries in early mouse embryo. J. Anat.201,15-29.","DOI":"10.1046\/j.1469-7580.2002.00071.x"},{"key":"2021042621221865300_REF20","doi-asserted-by":"crossref","unstructured":"Hosoda, K., Hammer, R. E., Richardson, J. A., Baynash, A. G.,Cheung, J. C., Giaid, A. and Yanagisawa, M. (1994). Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice. Cell79,1267-1276.","DOI":"10.1016\/0092-8674(94)90017-5"},{"key":"2021042621221865300_REF21","doi-asserted-by":"crossref","unstructured":"Kapur, R. P. (1999). Early death of neural crest cells is responsible for total enteric aganglionosis in Sox10(Dom)\/Sox10(Dom) mouse embryos. Pediatr. Dev. Pathol.2,559-569.","DOI":"10.1007\/s100249900162"},{"key":"2021042621221865300_REF22","doi-asserted-by":"crossref","unstructured":"Kos, R., Reedy, M. V., Johnson, R. L. and Erickson, C. A.(2001). The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos. Development128,1467-1479.","DOI":"10.1242\/dev.128.8.1467"},{"key":"2021042621221865300_REF23","doi-asserted-by":"crossref","unstructured":"Labosky, P. A. and Kaestner, K. H. (1998). The winged helix transcription factor Hfh2 is expressed in neural crest and spinal cord during mouse development. Mech. Dev.76,185-190.","DOI":"10.1016\/S0925-4773(98)00105-1"},{"key":"2021042621221865300_REF24","doi-asserted-by":"crossref","unstructured":"Le Douarin, N. M. and Kalcheim, C. (1999).The Neural Crest. Cambridge: Cambridge University Press.","DOI":"10.1017\/CBO9780511897948"},{"key":"2021042621221865300_REF25","doi-asserted-by":"crossref","unstructured":"Lee, H. C., Huang, H. Y., Lin, C. Y., Chen, Y. H. and Tsai, H. J. (2006). Foxd3 mediates zebrafish myf5 expression during early somitogenesis. Dev. Biol.290,359-372.","DOI":"10.1016\/j.ydbio.2005.11.032"},{"key":"2021042621221865300_REF26","doi-asserted-by":"crossref","unstructured":"Li, Y. X., Zdanowicz, M., Young, L., Kumiski, D., Leatherbury,L. and Kirby, M. L. (2003). Cardiac neural crest in zebrafish embryos contributes to myocardial cell lineage and early heart function. Dev. Dyn.226,540-550.","DOI":"10.1002\/dvdy.10264"},{"key":"2021042621221865300_REF27","doi-asserted-by":"crossref","unstructured":"Lister, J. A., Cooper, C., Nguyen, K., Modrell, M., Grant, K. and Raible, D. W. (2006). Zebrafish Foxd3 is required for development of a subset of neural crest derivatives. Dev. Biol.290,92-104.","DOI":"10.1016\/j.ydbio.2005.11.014"},{"key":"2021042621221865300_REF28","doi-asserted-by":"crossref","unstructured":"Mercola, M., Wang, C. Y., Kelly, J., Brownlee, C.,Jackson-Grusby, L., Stiles, C. and Bowen-Pope, D. (1990). Selective expression of PDGF A and its receptor during early mouse embryogenesis. Dev. Biol.138,114-122.","DOI":"10.1016\/0012-1606(90)90181-H"},{"key":"2021042621221865300_REF29","doi-asserted-by":"crossref","unstructured":"Montero-Balaguer, M., Lang, M. R., Sachdev, S. W., Knappmeyer,C., Stewart, R. A., De La Guardia, A., Hatzopoulos, A. K. and Knapik, E. W. (2006). The mother superior mutation ablates foxd3 activity in neural crest progenitor cells and depletes neural crest derivatives in zebrafish. Dev. Dyn.235,3199-3212.","DOI":"10.1002\/dvdy.20959"},{"key":"2021042621221865300_REF30","unstructured":"Nagy, A., Gertsenstein, M., Ventersten, K. and Behringer, R.(2003). Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press."},{"key":"2021042621221865300_REF31","doi-asserted-by":"crossref","unstructured":"Pardal, R., Molofsky, A. V., He, S. and Morrison, S. J.(2005). Stem cell self-renewal and cancer cell proliferation are regulated by common networks that balance the activation of proto-oncogenes and tumor suppressors. Cold Spring Harb. Symp. Quant. Biol.70,177-185.","DOI":"10.1101\/sqb.2005.70.057"},{"key":"2021042621221865300_REF32","doi-asserted-by":"crossref","unstructured":"Pattyn, A., Morin, X., Cremer, H., Goridis, C. and Brunet, J. F. (1999). The homeobox gene Phox2b is essential for the development of autonomic neural crest derivatives. Nature399,366-370.","DOI":"10.1038\/20700"},{"key":"2021042621221865300_REF33","doi-asserted-by":"crossref","unstructured":"Pichel, J. G., Shen, L., Sheng, H. Z., Granholm, A. C., Drago,J., Grinberg, A., Lee, E. J., Huang, S. P., Saarma, M., Hoffer, B. J. et al. (1996). Defects in enteric innervation and kidney development in mice lacking GDNF. Nature382, 73-76.","DOI":"10.1038\/382073a0"},{"key":"2021042621221865300_REF34","unstructured":"Presnell, J. K. and Schreibman, M. P. (1997).Humason's Animal Tissue Techniques. Baltimore, MD: The Johns Hopkins University Press."},{"key":"2021042621221865300_REF35","doi-asserted-by":"crossref","unstructured":"Sanchez, M. P., Silos-Santiago, I., Frisen, J., He, B., Lira, S. A. and Barbacid, M. (1996). Renal agenesis and the absence of enteric neurons in mice lacking GDNF. Nature382, 70-73.","DOI":"10.1038\/382070a0"},{"key":"2021042621221865300_REF36","unstructured":"Sasai, N., Mizuseki, K. and Sasai, Y. (2001). Requirement of FoxD3-class signaling for neural crest determination in Xenopus. Development128,2525-2536."},{"key":"2021042621221865300_REF37","doi-asserted-by":"crossref","unstructured":"Satokata, I. and Maas, R. (1994). Msx1 deficient mice exhibit cleft palate and abnormalities of craniofacial and tooth development. Nat. Genet.6, 348-356.","DOI":"10.1038\/ng0494-348"},{"key":"2021042621221865300_REF38","doi-asserted-by":"crossref","unstructured":"Schorle, H., Meier, P., Buchert, M., Jaenisch, R. and Mitchell,P. J. (1996). Transcription factor AP-2 essential for cranial closure and craniofacial development. Nature381,235-238.","DOI":"10.1038\/381235a0"},{"key":"2021042621221865300_REF39","doi-asserted-by":"crossref","unstructured":"Serbedzija, G. N., Burgan, S., Fraser, S. E. and Bronner-Fraser,M. (1991). Vital dye labelling demonstrates a sacral neural crest contribution to the enteric nervous system of chick and mouse embryos. Development111,857-866.","DOI":"10.1242\/dev.111.4.857"},{"key":"2021042621221865300_REF40","doi-asserted-by":"crossref","unstructured":"Soriano, P. (1999). Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat. Genet.21, 70-71.","DOI":"10.1038\/5007"},{"key":"2021042621221865300_REF41","doi-asserted-by":"crossref","unstructured":"Southard-Smith, E. M., Kos, L. and Pavan, W. J.(1998). Sox10 mutation disrupts neural crest development in Dom Hirschsprung mouse model. Nat. Genet.18, 60-64.","DOI":"10.1038\/ng0198-60"},{"key":"2021042621221865300_REF42","doi-asserted-by":"crossref","unstructured":"Steiner, A. B., Engleka, M. J., Lu, Q., Piwarzyk, E. C.,Yaklichkin, S., Lefebvre, J. L., Walters, J. W., Pineda-Salgado, L., Labosky,P. A. and Kessler, D. S. (2006). FoxD3 regulation of Nodal in the Spemann organizer is essential for Xenopus dorsal mesoderm development. Development133,4827-4838.","DOI":"10.1242\/dev.02663"},{"key":"2021042621221865300_REF43","doi-asserted-by":"crossref","unstructured":"Steventon, B., Carmona-Fontaine, C. and Mayor, R.(2005). Genetic network during neural crest induction: from cell specification to cell survival. Semin. Cell Dev. Biol.16,647-654.","DOI":"10.1016\/j.semcdb.2005.06.001"},{"key":"2021042621221865300_REF44","doi-asserted-by":"crossref","unstructured":"Stewart, R. A., Arduini, B. L., Berghmans, S., George, R. E.,Kanki, J. P., Henion, P. D. and Look, A. T. (2006). Zebrafish foxd3 is selectively required for neural crest specification, migration and survival. Dev. Biol.292,174-188.","DOI":"10.1016\/j.ydbio.2005.12.035"},{"key":"2021042621221865300_REF45","unstructured":"Stoner, C. M. and Gudas, L. J. (1989). Mouse cellular retinoic acid binding protein: cloning, complementary DNA sequence,and messenger RNA expression during the retinoic acid-induced differentiation of F9 wild type and RA-3-10 mutant teratocarcinoma cells. Cancer Res.49,1497-1504."},{"key":"2021042621221865300_REF46","doi-asserted-by":"crossref","unstructured":"Sutton, J., Costa, R., Klug, M., Field, L., Xu, D., Largaespada,D. A., Fletcher, C. F., Jenkins, N. A., Copeland, N. G., Klemsz, M. et al.(1996). Genesis, a winged helix transcriptional repressor with expression restricted to embryonic stem cells. J. Biol. Chem.271,23126-23133.","DOI":"10.1074\/jbc.271.38.23126"},{"key":"2021042621221865300_REF47","doi-asserted-by":"crossref","unstructured":"Takahashi, K. and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell126,663-676.","DOI":"10.1016\/j.cell.2006.07.024"},{"key":"2021042621221865300_REF48","doi-asserted-by":"crossref","unstructured":"Tompers, D. M. and Labosky, P. A. (2004). Electroporation of murine embryonic stem cells: a step-by-step guide. Stem Cells22,243-249.","DOI":"10.1634\/stemcells.22-3-243"},{"key":"2021042621221865300_REF49","doi-asserted-by":"crossref","unstructured":"Tompers, D. M., Foreman, R. K., Wang, Q., Kumanova, M. and Labosky, P. A. (2005). Foxd3 is required in the trophoblast progenitor cell lineage of the mouse embryo. Dev. Biol.285,126-137.","DOI":"10.1016\/j.ydbio.2005.06.008"},{"key":"2021042621221865300_REF50","doi-asserted-by":"crossref","unstructured":"Van Praagh, R. and Van Praagh, S. (1965). The anatomy of common aorticopulmonary trunk (truncus arteriosus communis) and its embryologic implications. A study of 57 necropsy cases. Am. J. Cardiol.16,406-425.","DOI":"10.1016\/0002-9149(65)90732-0"},{"key":"2021042621221865300_REF51","doi-asserted-by":"crossref","unstructured":"Vohra, B. P., Tsuji, K., Nagashimada, M., Uesaka, T., Wind, D.,Fu, M., Armon, J., Enomoto, H. and Heuckeroth, R. O. (2006). Differential gene expression and functional analysis implicate novel mechanisms in enteric nervous system precursor migration and neuritogenesis. Dev. Biol.298,259-271.","DOI":"10.1016\/j.ydbio.2006.06.033"},{"key":"2021042621221865300_REF52","unstructured":"Wall, N. A., Jones, C. M., Hogan, B. L. and Wright, C. V.(1992). Expression and modification of Hox 2.1 protein in mouse embryos. Mech. Dev.37,111-120."},{"key":"2021042621221865300_REF53","doi-asserted-by":"crossref","unstructured":"Wei, K., Chen, J., Akrami, K., Galbraith, G. C., Lopez, I. A. and Chen, F. (2007). Neural crest cell deficiency of c-myc causes skull and hearing defects. Genesis45,382-390.","DOI":"10.1002\/dvg.20304"},{"key":"2021042621221865300_REF54","doi-asserted-by":"crossref","unstructured":"Wijchers, P. J., Burbach, J. P. and Smidt, M. P.(2006). In control of biology: of mice, men and Foxes. Biochem. J.397,233-246.","DOI":"10.1042\/BJ20060387"},{"key":"2021042621221865300_REF55","doi-asserted-by":"crossref","unstructured":"Yaklichkin, S., Steiner, A. B., Lu, Q. and Kessler, D. S.(2007). FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus. J. Biol. Chem.282,2548-2557.","DOI":"10.1074\/jbc.M607412200"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/135\/9\/1615\/1538981\/1615.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/135\/9\/1615\/1538981\/1615.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T08:37:50Z","timestamp":1684312670000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/135\/9\/1615\/64940\/Requirement-for-Foxd3-in-the-maintenance-of-neural"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5,1]]},"references-count":55,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2008,5,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.012179","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2008,5,1]]}}}