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We provide evidence that the PCP protein Diego (Dgo) promotes the maintenance of apical localization of Flamingo (Fmi), an atypical Cadherin-family member, which itself is required for the apical localization of the other PCP factors. This function of Dgo is redundant with Prickle (Pk) and Strabismus (Stbm), and only appreciable in double mutant tissue. We show that the initial membrane association of Dgo depends on Fz, and that Dgo physically interacts with Stbm and Pk through its Ankyrin repeats, providing evidence for a PCP multiprotein complex. These interactions suggest a positive feedback loop initiated by Fz that results in the apical maintenance of other PCP factors through Fmi.<\/jats:p>","DOI":"10.1242\/dev.01317","type":"journal-article","created":{"date-parts":[[2004,8,12]],"date-time":"2004-08-12T00:13:24Z","timestamp":1092269604000},"page":"4467-4476","source":"Crossref","is-referenced-by-count":151,"title":["Diego interacts with Prickle and Strabismus\/Van Gogh to localize planar cell polarity complexes"],"prefix":"10.1242","volume":"131","author":[{"given":"Gishnu","family":"Das","sequence":"first","affiliation":[{"name":"Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, One Gustave L. 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