{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:35Z","timestamp":1787702435318,"version":"build-2784847793"},"reference-count":57,"publisher":"The Company of Biologists","issue":"19","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,10,1]]},"abstract":"<jats:p>Regulatory interferences at the iron transporter ferroportin 1 (Fpn1) cause transient defects in iron homeostasis and erythropoiesis in polycythaemia(Pcm) mutant mice. The present study identified decreased Fpn1 expression in placental syncytiotrophoblast cells at late gestation as the mechanism of neonatal iron deficiency in Pcm mutants. Tissue specificity of embryonic Fpn1 dysregulation was evident from concomitant decreases in Fpn1 mRNA and protein expression in placenta and liver, as opposed to upregulation of Fpn1 protein despite decreased transcript levels in spleen, implicating post-transcriptional regulation of Fpn1. Dysregulation of Fpn1 and decreased iron levels in Pcm mutant spleens correlated with apoptotic cell death in the stroma, resulting in a semidominant spleen regression. At 7 weeks of age, a transient increase in spleen size in Pcm heterozygotes reflected a transient erythropoietin-mediated polycythemia. Structurally, Pcm mutant spleens displayed a severe defect in red pulp formation, including disruption of the sinusoidal endothelium, as well as discrete defects in white pulp organization during postnatal development. Reduced functional competence of the Pcmmutant spleen was manifested by an impaired response to chemically induced hemolytic anemia. Thus, aberrant Fpn1 regulation and iron homeostasis interferes with development of the spleen stroma during embryogenesis,resulting in a novel defect in spleen architecture postnatally.<\/jats:p>","DOI":"10.1242\/dev.01342","type":"journal-article","created":{"date-parts":[[2004,9,2]],"date-time":"2004-09-02T06:49:53Z","timestamp":1094107793000},"page":"4871-4881","source":"Crossref","is-referenced-by-count":38,"title":["Dysregulation of ferroportin 1 interferes with spleen organogenesis in polycythaemia mice"],"prefix":"10.1242","volume":"131","author":[{"given":"Henry","family":"Mok","sequence":"first","affiliation":[{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine,Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine,Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef T.","family":"Prchal","sequence":"additional","affiliation":[{"name":"Division of Hematology, Baylor College of Medicine and Michael DeBakey VAH Medical Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pe\u0301ter","family":"Balogh","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, University of Pe\u0301cs,Pe\u0301cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armin","family":"Schumacher","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine,Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042605033467600_REF1","doi-asserted-by":"crossref","unstructured":"Abboud, S. and Haile, D. 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