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In the mutants, premature fusion of cranial sutures occurs at early postnatal stages. To elucidate the mechanism of craniosynostosis, we studied intramembranous ossification in Axin2-null mice. The calvarial osteoblast development is significantly affected by the Axin2 mutation. The Axin2mutant displays enhanced expansion of osteoprogenitors, accelerated ossification, stimulated expression of osteogenic markers and increases in mineralization. Inactivation of Axin2 promotes osteoblast proliferation and differentiation in vivo and in vitro. Furthermore, as the mammalian skull is formed from cranial skeletogenic mesenchyme, which is derived from mesoderm and neural crest, our data argue for a region-specific effect of Axin2 on neural crest dependent skeletogenesis. The craniofacial anomalies caused by the Axin2 mutation are mediated through activation of \u03b2-catenin signaling, suggesting a novel role for the Wnt pathway in skull morphogenesis.<\/jats:p>","DOI":"10.1242\/dev.01786","type":"journal-article","created":{"date-parts":[[2005,3,24]],"date-time":"2005-03-24T17:13:28Z","timestamp":1111684408000},"page":"1995-2005","source":"Crossref","is-referenced-by-count":312,"title":["The role of Axin2 in calvarial morphogenesis and craniosynostosis"],"prefix":"10.1242","volume":"132","author":[{"given":"Hsiao-Man Ivy","family":"Yu","sequence":"first","affiliation":[{"name":"Center for Oral Biology, Department of Biomedical Genetics, Abs Institute of Biomedical Sciences, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Jerchow","sequence":"additional","affiliation":[{"name":"Max Delbruck Center for Molecular Medicine, Robert-Rossle-Strasse 10, 13122 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzong-Jen","family":"Sheu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Oral Biology, Department of Biomedical Genetics, Abs Institute of Biomedical Sciences, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Costantini","sequence":"additional","affiliation":[{"name":"Department of Genetics and Development, College of Physicians and Surgeons,Columbia University, 701 West 168th Street, New York, NY 10032, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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