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The GFP reporter labels a distinct subset of retinal ganglion cells (RGCs),which project mainly into one of the four retinorecipient layers of the tectum and into a small subset of the extratectal arborization fields. In this transgenic line, we carried out an ENU-mutagenesis screen by scoring live zebrafish larvae for anatomical phenotypes. Thirteen recessive mutations in 12 genes were discovered. In one mutant, ddl, the majority of RGCs fail to differentiate. Three of the mutations, vrt, late and tard, delay the orderly ingrowth of retinal axons into the tectum. Two alleles of drg disrupt the layer-specific targeting of retinal axons. Three genes, fuzz, beyo and brek, are required for confinement of the tectal neuropil. Fasciculation within the optic tract and adhesion within the tectal neuropil are regulated by vrt, coma, bluk, clew and blin. The mutated genes are predicted to encode molecules essential for building the intricate neural architecture of the visual system.<\/jats:p>","DOI":"10.1242\/dev.01861","type":"journal-article","created":{"date-parts":[[2005,6,2]],"date-time":"2005-06-02T00:25:37Z","timestamp":1117671937000},"page":"2955-2967","source":"Crossref","is-referenced-by-count":297,"title":["A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection"],"prefix":"10.1242","volume":"132","author":[{"given":"Tong","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Physiology, University of California, San Francisco,Programs in Neuroscience, Genetics, Human Genetics, and Developmental Biology,1550 4th Street, San Francisco, CA 94158-2722, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Roeser","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of California, San Francisco,Programs in Neuroscience, Genetics, Human Genetics, and Developmental Biology,1550 4th Street, San Francisco, CA 94158-2722, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Staub","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of California, San Francisco,Programs in Neuroscience, Genetics, Human Genetics, and Developmental Biology,1550 4th Street, San Francisco, CA 94158-2722, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herwig","family":"Baier","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of California, San Francisco,Programs in Neuroscience, Genetics, Human Genetics, and Developmental Biology,1550 4th Street, San Francisco, CA 94158-2722, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042604532881900_REF1","doi-asserted-by":"crossref","unstructured":"Amemiya, C. 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