{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:16:58Z","timestamp":1790738218522,"version":"4.1.0"},"reference-count":45,"publisher":"The Company of Biologists","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,6,15]]},"abstract":"<jats:p>Signaling through vitamin A metabolites is indispensable for spermatogenesis, and disruption of retinoic acid receptor alpha (RAR\u03b1)function resulted in male sterility and aberrant spermatogenesis, which resembled vitamin A deficiency. Here we investigated the lineage- and cell-specific role of RAR\u03b1-mediated signaling during spermatogenesis using germ-cell transplantation and genetically manipulated mouse models. We demonstrated that RAR\u03b1-deficient germ-cell stem cells were able to repopulate germ-cell-depleted wild-type testes and initiate spermatogenesis;however, improper cellular associations and abnormal sperm formation were observed. We further generated RAR\u03b1-deficient mice that expressed RAR\u03b1-EGFP fusion protein uniquely in haploid germ cells. Strikingly, spermatid orientation, alignment and release, as well as sperm morphology, were normal and there was a partial rescue of sterility. These data provide the first direct evidence for a distinct requirement of RAR\u03b1-mediated retinoid signaling specifically in germ cells.<\/jats:p>","DOI":"10.1242\/dev.020040","type":"journal-article","created":{"date-parts":[[2009,5,22]],"date-time":"2009-05-22T17:33:42Z","timestamp":1243013622000},"page":"2091-2100","source":"Crossref","is-referenced-by-count":56,"title":["Expression of retinoic acid receptor alpha in the germline is essential for proper cellular association and spermiogenesis during spermatogenesis"],"prefix":"10.1242","volume":"136","author":[{"given":"Sanny S. W.","family":"Chung","sequence":"first","affiliation":[{"name":"Department of Genetics and Development, Columbia University Medical Center,New York, NY 10032, USA."},{"name":"Department of Obstetrics and Gynecology, Columbia University Medical Center,New York, NY 10032, USA."},{"name":"The Institute of Human Nutrition, Columbia University Medical Center, New York, NY 10032, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Genetics and Development, Columbia University Medical Center,New York, NY 10032, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debra J.","family":"Wolgemuth","sequence":"additional","affiliation":[{"name":"Department of Genetics and Development, Columbia University Medical Center,New York, NY 10032, USA."},{"name":"Department of Obstetrics and Gynecology, Columbia University Medical Center,New York, NY 10032, USA."},{"name":"The Institute of Human Nutrition, Columbia University Medical Center, New York, NY 10032, USA."},{"name":"The Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, NY 10032, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042621354917400_REF1","doi-asserted-by":"crossref","unstructured":"Akmal, K. 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