{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:23:22Z","timestamp":1791408202849,"version":"4.3.3"},"reference-count":39,"publisher":"The Company of Biologists","issue":"19","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,10,1]]},"abstract":"<jats:p>The definition of time-specific requirements for a developmental gene can pinpoint the processes within which the gene is involved and can reveal potential late functions in structures and organs that fail to develop in germline mutants. Here, we show the first systematic time-course deletion, in parallel with timed cell fate mapping, of a developmentally crucial gene, Tbx1, during mouse embryogenesis. Tbx1 mouse mutants model DiGeorge syndrome, a disorder of pharyngeal and cardiovascular development. Results revealed different time requirements for the development of individual structures, as well as multiple and time-distinct roles during the development of the same organ or system. We also show that Tbx1 is required throughout pharyngeal segmentation for the regulation of endoderm expansion,thus this is the first gene implicated directly in this process. A genetic-based blueprint of crucial developmental times for organs and systems should be a valuable asset for our understanding of birth defect pathogenesis.<\/jats:p>","DOI":"10.1242\/dev.02018","type":"journal-article","created":{"date-parts":[[2005,9,2]],"date-time":"2005-09-02T20:13:10Z","timestamp":1125691990000},"page":"4387-4395","source":"Crossref","is-referenced-by-count":140,"title":["Timed mutation and cell-fate mapping reveal reiterated roles of\n                    <i>Tbx1<\/i>\n                    during embryogenesis, and a crucial function during segmentation of the pharyngeal system via regulation of endoderm expansion"],"prefix":"10.1242","volume":"132","author":[{"given":"Huansheng","family":"Xu","sequence":"first","affiliation":[{"name":"Program in Cardiovascular Sciences, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cardiovascular Development, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Departments of Pediatrics (Cardiology), Baylor College of Medicine, Houston,TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Cerrato","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics (Cardiology), Baylor College of Medicine, Houston,TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Baldini","sequence":"additional","affiliation":[{"name":"Program in Cardiovascular Sciences, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cardiovascular Development, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Departments of Pediatrics (Cardiology), Baylor College of Medicine, Houston,TX 77030, USA"},{"name":"Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030,USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042605200561100_REF1","unstructured":"Albrecht, U., Eichele, G., Helms, J. A. and Lu, H. C.(1997). Visualization of gene expression patterns by in situ hybridization. In Molecular and Cellular Methods in Developmental Toxicology (ed. G. P. Daston), pp.23-48. New York: CRC Press."},{"key":"2021042605200561100_REF2","doi-asserted-by":"crossref","unstructured":"Baldini, A. (2002). DiGeorge syndrome: the use of model organisms to dissect complex genetics. Hum. Mol. Genet.11,2363-2369.","DOI":"10.1093\/hmg\/11.20.2363"},{"key":"2021042605200561100_REF3","doi-asserted-by":"crossref","unstructured":"Cai, C. L., Liang, X., Shi, Y., Chu, P. H., Pfaff, S. L., Chen,J. and Evans, S. (2003). Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart. Dev. Cell5, 877-889.","DOI":"10.1016\/S1534-5807(03)00363-0"},{"key":"2021042605200561100_REF4","doi-asserted-by":"crossref","unstructured":"Chapman, D. L., Garvey, N., Hancock, S., Alexiou, M., Agulnik,S. I., Gibson-Brown, J. 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Immunol.5,546-553.","DOI":"10.1038\/ni1064"},{"key":"2021042605200561100_REF7","doi-asserted-by":"crossref","unstructured":"Graham, A. (2001). The development and evolution of the pharyngeal arches. J. Anat.199,133-141.","DOI":"10.1046\/j.1469-7580.2001.19910133.x"},{"key":"2021042605200561100_REF8","doi-asserted-by":"crossref","unstructured":"Graham, A. and Smith, A. (2001). Patterning the pharyngeal arches. BioEssays23, 54-61.","DOI":"10.1002\/1521-1878(200101)23:1<54::AID-BIES1007>3.0.CO;2-5"},{"key":"2021042605200561100_REF9","doi-asserted-by":"crossref","unstructured":"Hayashi, S. and McMahon, A. P. (2002). Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation\/inactivation in the mouse. Dev. Biol.244,305-318.","DOI":"10.1006\/dbio.2002.0597"},{"key":"2021042605200561100_REF10","doi-asserted-by":"crossref","unstructured":"Hu, T., Yamagishi, H., Maeda, J., McAnally, J., Yamagishi, C. and Srivastava, D. 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A., Papaioannou, V. E. and Mallo, M. (2005). Tbx1 is required for proper neural crest migration and to stabilize spatial patterns during middle and inner ear development. Mech. Dev.122,199-212.","DOI":"10.1016\/j.mod.2004.10.004"},{"key":"2021042605200561100_REF24","doi-asserted-by":"crossref","unstructured":"Ohuchi, H., Hori, Y., Yamasaki, M., Harada, H., Sekine, K.,Kato, S. and Itoh, N. (2000). FGF10 acts as a major ligand for FGF receptor 2 IIIb in mouse multi-organ development. Biochem. Biophys. Res. Commun.277,643-649.","DOI":"10.1006\/bbrc.2000.3721"},{"key":"2021042605200561100_REF25","doi-asserted-by":"crossref","unstructured":"Piotrowski, T. and Nusslein-Volhard, C. (2000). The endoderm plays an important role in patterning the segmented pharyngeal region in zebrafish (Danio rerio). Dev. Biol.225,339-356.","DOI":"10.1006\/dbio.2000.9842"},{"key":"2021042605200561100_REF26","doi-asserted-by":"crossref","unstructured":"Piotrowski, T., Ahn, D. G., Schilling, T. 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Lancet362,1366-1373."}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/132\/19\/4387\/1511261\/4387.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/132\/19\/4387\/1511261\/4387.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T06:19:16Z","timestamp":1683181156000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/132\/19\/4387\/42974\/Timed-mutation-and-cell-fate-mapping-reveal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10,1]]},"references-count":39,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2005,10,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.02018","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1027896.334859","asserted-by":"object"}]},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2005,10,1]]}}}