{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:01:28Z","timestamp":1791219688885,"version":"4.1.0"},"reference-count":74,"publisher":"The Company of Biologists","issue":"22","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,11,15]]},"abstract":"<jats:p>We here report essential roles of the Bmp-binding protein crossveinless 2(Cv2; Bmper) in mouse organogenesis. In the null Cv2 mutant mouse,gastrulation occurs normally, but a number of defects are found in Cv2-expressing tissues such as the skeleton. Cartilage differentiation by Bmp4 treatment is reduced in cultured Cv2-\/- fibroblasts. Moreover, the defects in the vertebral column and eyes of the Cv2-\/- mouse are substantially enhanced by deleting one copy of the Bmp4 gene, suggesting a pro-Bmp role of Cv2 in the development of these organs. In addition, the Cv2-\/- mutant exhibits substantial defects in Bmp-dependent processes of internal organ formation, such as nephron generation in the kidney. This kidney hypoplasia is synergistically enhanced by the additional deletion of Kcp (Crim2) which encodes a pro-Bmp protein structurally related to Cv2. This study demonstrates essential pro-Bmp functions of Cv2 for locally restricted signal enhancement in multiple aspects of mammalian organogenesis.<\/jats:p>","DOI":"10.1242\/dev.02647","type":"journal-article","created":{"date-parts":[[2006,10,12]],"date-time":"2006-10-12T00:30:27Z","timestamp":1160613027000},"page":"4463-4473","source":"Crossref","is-referenced-by-count":111,"title":["Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis"],"prefix":"10.1242","volume":"133","author":[{"given":"Makoto","family":"Ikeya","sequence":"first","affiliation":[{"name":"Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN,Kobe 650-0047, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Kawada","sequence":"additional","affiliation":[{"name":"Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN,Kobe 650-0047, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kiyonari","sequence":"additional","affiliation":[{"name":"Laboratory for Animal Resources and Genetic Engineering, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriaki","family":"Sasai","sequence":"additional","affiliation":[{"name":"Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN,Kobe 650-0047, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Nakao","sequence":"additional","affiliation":[{"name":"Laboratory for Animal Resources and Genetic Engineering, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhide","family":"Furuta","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, M. D. Anderson Cancer Center, University of Texas, Houston, TX 77030, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Sasai","sequence":"additional","affiliation":[{"name":"Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN,Kobe 650-0047, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042612213066700_REF1","doi-asserted-by":"crossref","unstructured":"Aruga, J., Mizugishi, K., Koseki, H., Imai, K., Balling, R.,Noda, T. and Mikoshiba, K. (1999). Zic1 regulates the patterning of vertebral arches in cooperation with Gli3. Mech. Dev.89,141-150.","DOI":"10.1016\/S0925-4773(99)00220-8"},{"key":"2021042612213066700_REF2","doi-asserted-by":"crossref","unstructured":"Ashe, H. L. and Levine, M. (1999). Local inhibition and long-range enhancement of Dpp signal transduction by Sog. Nature398,427-431.","DOI":"10.1038\/18892"},{"key":"2021042612213066700_REF3","doi-asserted-by":"crossref","unstructured":"Bachiller, D., Klingensmith, J., Shneyder, N., Tran, U.,Anderson, R., Rossant, J. and De Robertis, E. M. (2003). The role of chordin\/Bmp signals in mammalian pharyngeal development and DiGeorge syndrome. Development130,3567-3578.","DOI":"10.1242\/dev.00581"},{"key":"2021042612213066700_REF4","doi-asserted-by":"crossref","unstructured":"Binnerts, M. E., Wen, X., Cante-Barrett, K., Bright, J., Chen,H. T., Asundi, V., Sattari, P., Tang, T., Boyle, B., Funk, W. et al.(2004). Human Crossveinless-2 is a novel inhibitor of bone morphogenetic proteins. Biochem. Biophys. Res. Commun.315,272-280.","DOI":"10.1016\/j.bbrc.2004.01.048"},{"key":"2021042612213066700_REF5","doi-asserted-by":"crossref","unstructured":"Canalis, E., Economides, A. N. and Gazzerro, E.(2003). Bone morphogenetic proteins, their antagonists, and the skeleton. Endocr. Rev.24,218-235.","DOI":"10.1210\/er.2002-0023"},{"key":"2021042612213066700_REF6","unstructured":"Cheah, K. S., Lau, E. T., Au, P. K. and Tam, P. P.(1991). Expression of the mouse alpha 1(II) collagen gene is not restricted to cartilage during development. Development111,945-953."},{"key":"2021042612213066700_REF7","unstructured":"Coffinier, C., Ketpura, N., Tran, U., Geissert, D. and De Robertis, E. M. (2002). Mouse Crossveinless-2 is the vertebrate homolog of a Drosophila extracellular regulator of BMP signaling. Mech. Dev.119,S179-S184."},{"key":"2021042612213066700_REF8","doi-asserted-by":"crossref","unstructured":"Coles, E., Christiansen, J., Economou, A., Bronner-Fraser, M. and Wilkinson, D. G. (2004). A vertebrate crossveinless 2 homologue modulates BMP activity and neural crest cell migration. Development131,5309-5317.","DOI":"10.1242\/dev.01419"},{"key":"2021042612213066700_REF9","doi-asserted-by":"crossref","unstructured":"Conley, C. A., Silburn, R., Singer, M. A., Ralston, A.,Rohwer-Nutter, D., Olson, D. J., Gelbart, W. and Blair, S. S.(2000). Crossveinless 2 contains cysteine-rich domains and is required for high levels of BMP-like activity during the formation of the cross veins in Drosophila. Development127,3947-3959.","DOI":"10.1242\/dev.127.18.3947"},{"key":"2021042612213066700_REF10","doi-asserted-by":"crossref","unstructured":"Dale, L., Howes, G., Price, B. M. J. and Smith, J. C.(1992). Bone morphogenetic protein 4, a ventralizing factor in early Xenopus development. Development115,573-585.","DOI":"10.1242\/dev.115.2.573"},{"key":"2021042612213066700_REF11","unstructured":"Decotto, E. and Ferguson, E. L. (2001). A positive role for Short gastrulation in modulating BMP signaling during dorsoventral patterning in the Drosophila embryo. Development128,3831-3841."},{"key":"2021042612213066700_REF12","doi-asserted-by":"crossref","unstructured":"Delot, E. C., Bahamonde, M. E., Zhao, M. and Lyons, K. M.(2003). BMP signaling is required for septation of the outflow tract of the mammalian heart. Development130,209-220.","DOI":"10.1242\/dev.00181"},{"key":"2021042612213066700_REF13","doi-asserted-by":"crossref","unstructured":"De Robertis, E. M. and Sasai, Y. (1996). A common plan for dorsoventral patterning in Bilateria. Nature380,37-40.","DOI":"10.1038\/380037a0"},{"key":"2021042612213066700_REF14","doi-asserted-by":"crossref","unstructured":"Dudley, A. T., Lyons, K. M. and Robertson, E. J.(1995). A requirement for bone morphogenetic protein-7 during development of the mammalian kidney and eye. Genes Dev.9,2795-2807.","DOI":"10.1101\/gad.9.22.2795"},{"key":"2021042612213066700_REF15","doi-asserted-by":"crossref","unstructured":"Fainsod, A., Steinbeisser, H. and De Robertis, E. M.(1994). On the function of BMP-4 in patterning the marginal zone of the Xenopus embryo. EMBO J.13,5015-5025.","DOI":"10.1002\/j.1460-2075.1994.tb06830.x"},{"key":"2021042612213066700_REF16","doi-asserted-by":"crossref","unstructured":"Ferguson, E. L. and Anderson, K. (1992a). decapentaplegic acts as a morphogen to organize dorsal-ventral pattern in the Drosophila embryo. Cell71,451-461.","DOI":"10.1016\/0092-8674(92)90514-D"},{"key":"2021042612213066700_REF17","doi-asserted-by":"crossref","unstructured":"Ferguson, E. L. and Anderson, K. (1992b). Localized enhancement and repression of the activity of the TGF-beta family member, decapentaplegic, is necessary for dorso-ventral pattern formation in the Drosophila embryo. Development114,583-597.","DOI":"10.1242\/dev.114.3.583"},{"key":"2021042612213066700_REF18","unstructured":"Fisher, S., Amacher, S. L. and Halpern, M. E.(1997). Loss of cerebrum function ventralizes the zebrafish embryo. Development124,1301-1311."},{"key":"2021042612213066700_REF19","doi-asserted-by":"crossref","unstructured":"Franc\u0327ois, V., Solloway, M., O'Neill, J. W., Emery, J. and Bier, E. (1994). Dorsalventral patterning of the Drosophila embryo depends on a putative negative growth factor encoded by the shortgastrulation gene. Genes Dev.8,2602-2616.","DOI":"10.1101\/gad.8.21.2602"},{"key":"2021042612213066700_REF20","doi-asserted-by":"crossref","unstructured":"Garcia-Bellido, A. and de Celis, J. F. (1992). Developmental genetics of the venation pattern of Drosophila. Annu. Rev. Genet.26,277-304.","DOI":"10.1146\/annurev.ge.26.120192.001425"},{"key":"2021042612213066700_REF21","doi-asserted-by":"crossref","unstructured":"Glinka, A., Wu, W., Onichtchouk, D., Blumenstock, C. and Niehrs,C. (1997). Head induction by simultaneous repression of Bmp and Wnt signalling in Xenopus. Nature389,517-519.","DOI":"10.1038\/39092"},{"key":"2021042612213066700_REF22","doi-asserted-by":"crossref","unstructured":"Glumoff, V., Savontaus, M., Vehanen, J. and Vuorio, E.(1994). Analysis of aggrecan and tenascin gene expression in mouse skeletal tissues by northern and in situ hybridization using species specific cDNA probes. Biochim. Biophys. Acta1219,613-622.","DOI":"10.1016\/0167-4781(94)90220-8"},{"key":"2021042612213066700_REF23","doi-asserted-by":"crossref","unstructured":"Godin, R. E., Takaesu, N. T., Robertson, E. J. and Dudley, A. T. (1998). Regulation of BMP7 expression during kidney development. Development125,3473-3482.","DOI":"10.1242\/dev.125.17.3473"},{"key":"2021042612213066700_REF24","unstructured":"Godin, R. E., Robertson, E. J. and Dudley, A. T.(1999). Role of BMP family members during kidney development. Int. J. Dev. Biol.43,405-411."},{"key":"2021042612213066700_REF25","unstructured":"Goldman, D. C., Hackenmiller, R., Nakayama, T., Sopory, S.,Wong, C., Kulessa, H. and Christian, J. L. (2006). Mutation of an upstream cleavage site in the BMP4 prodomain leads to tissue-specific loss of activity. Development133,1933-1942."},{"key":"2021042612213066700_REF26","doi-asserted-by":"crossref","unstructured":"Grieshammer, U., Cebrian, C., Ilagan, R., Meyers, E.,Herzlinger, D. and Martin, G. R. (2005). FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons. Development132,3847-3857.","DOI":"10.1242\/dev.01944"},{"key":"2021042612213066700_REF27","doi-asserted-by":"crossref","unstructured":"Gurdon, J. B. and Bourillot, P. Y. (2001). Morphogen gradient interpretation. Nature413,797-803.","DOI":"10.1038\/35101500"},{"key":"2021042612213066700_REF28","doi-asserted-by":"crossref","unstructured":"Hammerschmidt, M. and Mullins, M. C. (2002). Dorsoventral patterning in the zebrafish: bone morphogenetic proteins and beyond. In Pattern Formation in Zebrafish (ed. L. Solnica-Krezel), pp. 72-95. Berlin, Heidelberg:Springer-Verlag","DOI":"10.1007\/978-3-540-46041-1_5"},{"key":"2021042612213066700_REF29","doi-asserted-by":"crossref","unstructured":"Hemmati-Brivanlou, A., Kelly, O. G. and Melton, D. A.(1994). Follistatin, an antagonist of activin is expressed in the Spemann organizer and displays direct neuralizing activity. Cell77,283-295.","DOI":"10.1016\/0092-8674(94)90320-4"},{"key":"2021042612213066700_REF30","doi-asserted-by":"crossref","unstructured":"Hogan, B. L. (1996). Bone morphogenetic proteins: multifunctional regulators of vertebrate development. Genes Dev.10,1580-1594.","DOI":"10.1101\/gad.10.13.1580"},{"key":"2021042612213066700_REF31","unstructured":"Hogan, B., Beddington, R., Costantini, F. and Lacy, E.(1994). Manipulating the Mouse Embryo(2nd edn). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press."},{"key":"2021042612213066700_REF32","doi-asserted-by":"crossref","unstructured":"Hsu, D. R., Economides, A. N., Wang, X., Eimon, P. M. and Harland, R. M. (1998). The Xenopus dorsalizing factor Gremlin identifies a novel family of secreted proteins that antagonize BMP activities. Mol. Cell1,673-683.","DOI":"10.1016\/S1097-2765(00)80067-2"},{"key":"2021042612213066700_","doi-asserted-by":"crossref","unstructured":"Ikeya, M. and Takada, S. (1998). Wnt signaling from the dorsal neural tube is required for the formation of the medial dermomyotome. Development125,4969-4976.","DOI":"10.1242\/dev.125.24.4969"},{"key":"2021042612213066700_REF34","doi-asserted-by":"crossref","unstructured":"Ikeya, M., Kawada, M., Nakazawa, Y., Sakuragi, M., Sasai, N.,Ueno, M., Kiyonari, H., Nakao, K. and Sasai, Y. (2005). Gene disruption\/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations. Int. J. Dev. Biol.49,807-823.","DOI":"10.1387\/ijdb.051975mi"},{"key":"2021042612213066700_REF35","doi-asserted-by":"crossref","unstructured":"Jena, N., Martin-Seisdedos, C., McCue, P. and Croce, C. M.(1997). BMP7 null mutation in mice: developmental defects in skeleton, kidney, and eye. Exp. Cell Res.230, 28-37.","DOI":"10.1006\/excr.1996.3411"},{"key":"2021042612213066700_REF36","doi-asserted-by":"crossref","unstructured":"Jones, C. M., Lyons, K. M., Lapan, P. M., Wright, C. V. E. and Hogan, B. L. M. (1992). DVR-4 (bone morphogenetic protein-4)as a posterior-ventralizing factor in Xenopus mesoderm induction. Development115,639-647.","DOI":"10.1242\/dev.115.2.639"},{"key":"2021042612213066700_REF37","doi-asserted-by":"crossref","unstructured":"Kamimura, M., Matsumoto, K., Koshiba-Takeuchi, K. and Ogura,T. (2004). Vertebrate crossveinless 2 is secreted and acts as an extracellular modulator of the BMP signaling cascade. Dev. Dyn.230,434-445.","DOI":"10.1002\/dvdy.20069"},{"key":"2021042612213066700_REF38","doi-asserted-by":"crossref","unstructured":"Lamb, T. M., Knecht, A. K., Smith, W. C., Stachel, S. E.,Economides, A. N., Stahl, N., Yancopolous, G. D. and Harland, R. M.(1993). Neural induction by the secreted polypeptide noggin. Science262,713-718.","DOI":"10.1126\/science.8235591"},{"key":"2021042612213066700_REF39","doi-asserted-by":"crossref","unstructured":"Larrain, J., Bachiller, D., Lu, B., Agius, E., Piccolo, S. and De Robertis, E. M. (2000). BMP-binding modules in chordin: a model for signalling regulation in the extracellular space. Development127,821-830.","DOI":"10.1242\/dev.127.4.821"},{"key":"2021042612213066700_REF40","doi-asserted-by":"crossref","unstructured":"Lawson, K. A., Dunn, N. R., Roelen, B. A., Zeinstra, L. M.,Davis, A. M., Wright, C. V., Korving, J. P. and Hogan, B. L.(1999). Bmp4 is required for the generation of primordial germ cells in the mouse embryo. Genes Dev.13,424-436.","DOI":"10.1101\/gad.13.4.424"},{"key":"2021042612213066700_REF41","doi-asserted-by":"crossref","unstructured":"Lee, K. J. and Jessell, T. M. (1999). The specification of dorsal cell fates in the vertebrate central nervous system. Annu. Rev. Neurosci.22,261-294.","DOI":"10.1146\/annurev.neuro.22.1.261"},{"key":"2021042612213066700_REF42","doi-asserted-by":"crossref","unstructured":"Lengner, C. J., Lepper, C., van Wijnen, A. J., Stein, J. L.,Stein, G. S. and Lian, J. B. (2004). Primary mouse embryonic fibroblasts: a model of mesenchymal cartilage formation. J. Cell Physiol.200,327-333.","DOI":"10.1002\/jcp.20118"},{"key":"2021042612213066700_REF43","doi-asserted-by":"crossref","unstructured":"Lin, J., Patel, S. R., Cheng, X., Cho, E. A., Levitan, I.,Ullenbruch, M., Phan, S. H., Park, J. M. and Dressler, G. R.(2005). Kielin\/chordin-like protein, a novel enhancer of BMP signaling, attenuates renal fibrotic disease. Nat. Med.11,387-393.","DOI":"10.1038\/nm1217"},{"key":"2021042612213066700_REF44","doi-asserted-by":"crossref","unstructured":"Luo, G., Hofmann, C., Bronckers, A. L., Sohocki, M., Bradley, A. and Karsenty, G. (1995). BMP-7 is an inducer of nephrogenesis, and is also required for eye development and skeletal patterning. Genes Dev.9,2808-2820.","DOI":"10.1101\/gad.9.22.2808"},{"key":"2021042612213066700_REF45","doi-asserted-by":"crossref","unstructured":"Massague, J. and Chen, Y. G. (2000). Controlling TGF-beta signaling. Genes Dev.14,627-644.","DOI":"10.1101\/gad.14.6.627"},{"key":"2021042612213066700_REF46","doi-asserted-by":"crossref","unstructured":"Matsui, M., Mizuseki, K., Nakatani, J., Nakanishi, S. and Sasai,Y. (2000). Xenopus kielin: A dorsalizing factor containing multiple chordin-type repeats secreted from the embryonic midline. Proc. Natl. Acad. Sci. USA97,5291-5296.","DOI":"10.1073\/pnas.090020497"},{"key":"2021042612213066700_REF47","doi-asserted-by":"crossref","unstructured":"Miyazaki, Y., Oshima, K., Fogo, A., Hogan, B. L. and Ichikawa,I. (2000). Bone morphogenetic protein 4 regulates the budding site and elongation of the mouse ureter. J. Clin. Invest.105,863-873.","DOI":"10.1172\/JCI8256"},{"key":"2021042612213066700_REF48","doi-asserted-by":"crossref","unstructured":"Mizuseki, K., Sakamoto, T., Watanabe, K., Muguruma, K., Ikeya,M., Nishiyama, A., Arakawa, A., Suemori, H., Nakatsuji, N., Kawasaki, H. et al. (2003). Generation of neural crest-derived peripheral neurons and floor plate cells from mouse and primate embryonic stem cells. Proc. Natl. Acad. Sci. USA100,5828-5833.","DOI":"10.1073\/pnas.1037282100"},{"key":"2021042612213066700_REF49","doi-asserted-by":"crossref","unstructured":"Moser, M., Binder, O., Wu, Y., Aitsebaomo, J., Ren, R., Bode,C., Bautch, V. L., Conlon, F. L. and Patterson, C. (2003). BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation. Mol. Cell. Biol.23,5664-5679.","DOI":"10.1128\/MCB.23.16.5664-5679.2003"},{"key":"2021042612213066700_REF50","doi-asserted-by":"crossref","unstructured":"Murtaugh, L. C., Chyung, J. H. and Lassar, A. B.(1999). Sonic hedgehog promotes somitic chondrogenesis by altering the cellular response to BMP signaling. Genes Dev.13,225-237.","DOI":"10.1101\/gad.13.2.225"},{"key":"2021042612213066700_REF51","doi-asserted-by":"crossref","unstructured":"Nosaka, T., Morita, S., Kitamura, H., Nakajima, H., Shibata, F.,Morikawa, Y., Kataoka, Y., Ebihara, Y., Kawashima, T., Itoh, T. et al.(2003). Mammalian twisted gastrulation is essential for skeleto-lymphogenesis. Mol. Cell. Biol.23,2969-2980.","DOI":"10.1128\/MCB.23.8.2969-2980.2003"},{"key":"2021042612213066700_REF52","unstructured":"O'Connor, M. B., Umulis, D., Othmer, H. G. and Blair, S. S.(2006). Shaping BMP morphogen gradients in the Drosophila embryo and pupal wing. Development133,183-193."},{"key":"2021042612213066700_REF53","doi-asserted-by":"crossref","unstructured":"Parr, B. A. and McMahon, A. P. (1995). Dorsalizing signal Wnt-7a required for normal polarity of D-V and A-P axes of mouse limb. Nature374,350-353.","DOI":"10.1038\/374350a0"},{"key":"2021042612213066700_REF54","doi-asserted-by":"crossref","unstructured":"Petryk, A., Anderson, R. M., Jarcho, M. P., Leaf, I., Carlson,C. S., Klingensmith, J., Shawlot, W. and O'Connor, M. B.(2004). The mammalian twisted gastrulation gene functions in foregut and craniofacial development. Dev. Biol.267,374-386.","DOI":"10.1016\/j.ydbio.2003.11.015"},{"key":"2021042612213066700_REF55","doi-asserted-by":"crossref","unstructured":"Ralston, A. and Blair, S. S. (2005). Long-range Dpp signaling is regulated to restrict BMP signaling to a crossvein competent zone. Dev. Biol.280,187-200.","DOI":"10.1016\/j.ydbio.2005.01.018"},{"key":"2021042612213066700_REF56","doi-asserted-by":"crossref","unstructured":"Rentzsch, F., Zhang, J., Kramer, C., Sebald, W. and Hammerschmidt, M. (2006). Crossveinless 2 is an essential positive feedback regulator of Bmp signaling during zebrafish gastrulation. Development133,801-811.","DOI":"10.1242\/dev.02250"},{"key":"2021042612213066700_REF57","doi-asserted-by":"crossref","unstructured":"Sasai, Y., Lu, B., Steinbeisser, H., Geissert, D., Gont, L. K. and De Robertis, E. M. (1994). Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeoboxes genes. Cell79,779-790.","DOI":"10.1016\/0092-8674(94)90068-X"},{"key":"2021042612213066700_REF58","doi-asserted-by":"crossref","unstructured":"Sasai, Y., Lu, B., Steinbeisser, H. and De Robertis, E. M.(1995). Regulation of neural induction by the chd and BMP-4 antagonistic patterning signals in Xenopus. Nature376,333-336.","DOI":"10.1038\/376333a0"},{"key":"2021042612213066700_REF59","doi-asserted-by":"crossref","unstructured":"Schulte-Merker, S., Lee, K. J., McMahon, A. P. and Hammerschmidt, M. (1997). The zebrafish organizer requires chordino. Nature387,862-863.","DOI":"10.1038\/43092"},{"key":"2021042612213066700_REF60","doi-asserted-by":"crossref","unstructured":"Simic, P. and Vukicevic, S. (2005). Bone morphogenetic proteins in development and homeostasis of kidney. Cytokine Growth Factor Rev.16,299-308.","DOI":"10.1016\/j.cytogfr.2005.02.010"},{"key":"2021042612213066700_REF61","doi-asserted-by":"crossref","unstructured":"Smith, W. C. and Harland, R. M. (1992). Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos. Cell70,829-840.","DOI":"10.1016\/0092-8674(92)90316-5"},{"key":"2021042612213066700_REF62","doi-asserted-by":"crossref","unstructured":"Su, H. L., Muguruma, K., Matsuo-Takasaki, M., Kengaku, M.,Watanabe, K. and Sasai, Y. (2006). Generation of cerebellar neuron precursors from embryonic stem cells. Dev. Biol.290,287-296.","DOI":"10.1016\/j.ydbio.2005.11.010"},{"key":"2021042612213066700_REF63","doi-asserted-by":"crossref","unstructured":"Suzuki, N., Labosky, P. A., Furuta, Y., Hargett, L., Dunn, R.,Fogo, A. B., Takahara, K., Peters, D. M., Greenspan, D. S. and Hogan, B. L. (1996). Failure of ventral body wall closure in mouse embryos lacking a procollagen C-proteinase encoded by Bmp1, a mammalian gene related to Drosophila tolloid. Development122,3587-3595.","DOI":"10.1242\/dev.122.11.3587"},{"key":"2021042612213066700_REF64","doi-asserted-by":"crossref","unstructured":"Wagner, D. S. and Mullins, M. C. (2002). Modulation of BMP activity in dorsalventral pattern formation by the chordin and ogon antagonists. Dev. Biol.245,109-123.","DOI":"10.1006\/dbio.2002.0614"},{"key":"2021042612213066700_REF65","doi-asserted-by":"crossref","unstructured":"Wan, M. and Cao, X. (2005). BMP signaling in skeletal development. Biochem. Biophys. Res. Commun.328,651-657.","DOI":"10.1016\/j.bbrc.2004.11.067"},{"key":"2021042612213066700_REF66","doi-asserted-by":"crossref","unstructured":"Weaver, M., Yingling, J. M., Dunn, N. R., Bellusci, S. and Hogan, B. L. (1999). Bmp signaling regulates proximal-distal differentiation of endoderm in mouse lung development. Development126,4005-4015.","DOI":"10.1242\/dev.126.18.4005"},{"key":"2021042612213066700_REF67","doi-asserted-by":"crossref","unstructured":"Weaver, M., Batts, L. and Hogan, B. L. (2003). Tissue interactions pattern the mesenchyme of the embryonic mouse lung. Dev. Biol.258,169-184.","DOI":"10.1016\/S0012-1606(03)00117-9"},{"key":"2021042612213066700_REF68","doi-asserted-by":"crossref","unstructured":"Wharton, K. A., Ray, R. P. and Gelbart, W. M.(1993). The activity gradient of decapentaplegic is necessary for the specification of dorsal pattern elements in the Drosophila embryo. Development117,807-822.","DOI":"10.1242\/dev.117.2.807"},{"key":"2021042612213066700_REF69","doi-asserted-by":"crossref","unstructured":"Yagi, T., Tokunaga, T., Furuta, Y., Nada, S., Yoshida, M.,Tsukada, T., Saga, Y., Takeda, N., Ikawa, Y. and Aizawa, S.(1993a). A novel ES cell line, TT2, with high germline-differentiating potency. Anal. Biochem.214, 70-76.","DOI":"10.1006\/abio.1993.1458"},{"key":"2021042612213066700_REF70","doi-asserted-by":"crossref","unstructured":"Yagi, T., Nada, S., Watanabe, N., Tamemoto, H., Kohmura, N.,Ikawa, Y. and Aizawa, S. (1993b). A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene. Anal. Biochem.214,77-86.","DOI":"10.1006\/abio.1993.1459"},{"key":"2021042612213066700_REF71","doi-asserted-by":"crossref","unstructured":"Yi, S. E., Daluiski, A., Pederson, R., Rosen, V. and Lyons, K. M. (2000). The type I BMP receptor BMPRIB is required for chondrogenesis in the mouse limb. Development127,621-630.","DOI":"10.1242\/dev.127.3.621"},{"key":"2021042612213066700_REF72","doi-asserted-by":"crossref","unstructured":"Zakin, L. and De Robertis, E. M. (2004). Inactivation of mouse Twisted gastrulation reveals its role in promoting Bmp4 activity during forebrain development. Development131,413-424.","DOI":"10.1242\/dev.00946"},{"key":"2021042612213066700_REF73","doi-asserted-by":"crossref","unstructured":"Zakin, L., Reversade, B., Kuroda, H., Lyons, K. M. and De Robertis, E. M. (2005). Sirenomelia in Bmp7 and Tsg compound mutant mice: requirement for Bmp signaling in the development of ventral posterior mesoderm. Development132,2489-2499.","DOI":"10.1242\/dev.01822"},{"key":"2021042612213066700_REF74","doi-asserted-by":"crossref","unstructured":"Zusman, S. B., Sweeton, D. and Wieschaus, E. F.(1988). short-gastrulation, a mutation causing delays in stage-specific cell shape changes during gastrulation in Drosophila melanogaster. Dev. Biol.129,417-442.","DOI":"10.1016\/0012-1606(88)90389-2"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/133\/22\/4463\/1515845\/4463.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/133\/22\/4463\/1515845\/4463.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T09:21:49Z","timestamp":1683624109000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/133\/22\/4463\/52864\/Essential-pro-Bmp-roles-of-crossveinless-2-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,15]]},"references-count":74,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2006,11,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.02647","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2006,11,15]]}}}