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It has been known for some time that the early mouse embryo metabolises external pyruvate and lactate but not glucose to produce ATP. We now show at the level of single oocytes, that supplied glucose has no effect on the redox potential of the oocyte. Pyruvate is a cytosolic oxidant but a mitochondrial reductant,while lactate is a strong cytosolic reductant via the activity of lactate dehydrogenase. Unexpectedly, lactate-derived pyruvate appears to be diverted from mitochondrial oxidation. Our approach also reveals that the level of reduced glutathione (GSH) in the oocyte is maintained by glutathione reductase, which oxidises intracellular NADPH to reduce oxidised glutathione. Surprisingly, NADPH does not seem to be supplied by the pentose phosphate pathway in the unfertilised oocyte but rather by cytosolic NADP-dependent isocitrate dehydrogenase. Remarkably, we also found that the oxidant action of pyruvate impairs development, demonstrating the fundamental importance of redox state on early development.<\/jats:p>","DOI":"10.1242\/dev.02744","type":"journal-article","created":{"date-parts":[[2006,12,22]],"date-time":"2006-12-22T01:14:06Z","timestamp":1166750046000},"page":"455-465","source":"Crossref","is-referenced-by-count":222,"title":["Regulation of redox metabolism in the mouse oocyte and embryo"],"prefix":"10.1242","volume":"134","author":[{"given":"Re\u0301mi","family":"Dumollard","sequence":"first","affiliation":[{"name":"Laboratoire de Biologie du De\u0301veloppement UMR 7009 CNRS\/Paris VI,Observatoire, Station Zoologique, Villefranche sur Mer, 06230 France."},{"name":"Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Ward","sequence":"additional","affiliation":[{"name":"Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Carroll","sequence":"additional","affiliation":[{"name":"Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Duchen","sequence":"additional","affiliation":[{"name":"Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042612273123700_REF1","doi-asserted-by":"crossref","unstructured":"Akella, S. 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