{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:32:22Z","timestamp":1791217942411,"version":"4.1.0"},"reference-count":67,"publisher":"The Company of Biologists","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,2,1]]},"abstract":"<jats:p>Worker termites of the genus Reticulitermes are temporally-arrested juvenile forms that can terminally differentiate into adultsoldier- or reproductive-caste phenotypes. Soldier-caste differentiation is a developmental transition that is induced by high juvenile hormone (JH)titers. Recently, a status quo hexamerin mechanism was identified, which reduces JH efficacy and maximizes colony fitness via the maintenance of high worker-caste proportions. Our goal in these studies was to investigate more thoroughly the influences of the hexamerins on JH-dependent gene expression in termite workers. Our approach involved RNA interference (RNAi), bioassays and quantification of gene expression. We first investigated the expression of 17 morphogenesis-associated genes in response to RNAi-based hexamerin silencing. Hexamerin silencing resulted in significant downstream impacts on 15 out of the 17 genes, suggesting that these genes are members of a JH-responsive genomic network. Next, we compared gene-expression profiles in workers after RNAi-based hexamerin silencing to that of (i) untreated workers that were held away from the colony; and (ii) workers that were also held away from the colony, but with ectopic JH. Here, although there was no correlation between hexamerin silencing and colony-release effects, we observed a significant correlation between hexamerin silencing and JH-treatment effects. These findings provide further evidence supporting the hypothesis that the hexamerins modulate JH availability, thus limiting the impacts of JH on termite caste polyphenism. Results are discussed in a context relative to outstanding questions on termite developmental biology, particularly on regulatory gene networks that respond to JH-, colony- and environmental-cues.<\/jats:p>","DOI":"10.1242\/dev.02755","type":"journal-article","created":{"date-parts":[[2007,1,10]],"date-time":"2007-01-10T22:53:29Z","timestamp":1168469609000},"page":"601-610","source":"Crossref","is-referenced-by-count":109,"title":["Hexamerin-based regulation of juvenile hormone-dependent gene expression underlies phenotypic plasticity in a social insect"],"prefix":"10.1242","volume":"134","author":[{"given":"Xuguo","family":"Zhou","sequence":"first","affiliation":[{"name":"Entomology and Nematology Department, University of Florida, Gainesville,FL 32611-0620, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"Tarver","sequence":"additional","affiliation":[{"name":"Entomology and Nematology Department, University of Florida, Gainesville,FL 32611-0620, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Scharf","sequence":"additional","affiliation":[{"name":"Entomology and Nematology Department, University of Florida, Gainesville,FL 32611-0620, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042612274029300_REF1","doi-asserted-by":"crossref","unstructured":"Andersen, C. 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