{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T07:01:15Z","timestamp":1792479675820,"version":"4.5.0"},"reference-count":38,"publisher":"The Company of Biologists","issue":"15","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,8,1]]},"abstract":"<jats:p>Although purines and purinergic signaling are crucial for numerous biochemical and cellular processes, their functions during vertebrate embryonic development have not been well characterized. We analyze two recessive zebrafish mutations that affect de novo purine synthesis, gart and paics. gart encodes phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase,phosphoribosylaminoimidazole synthetase, a trifunctional enzyme that catalyzes steps 2, 3 and 5 of inosine monophosphate (IMP) synthesis. paicsencodes phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase, a bifunctional enzyme that catalyzes steps 6 and 7 of this process. Zygotic gart and paics mutants have pigmentation defects in which xanthophore and iridophore pigmentation is almost completely absent, and melanin-derived pigmentation is significantly decreased, even though pigment cells are present in normal amounts and distributions. Zygotic gart and paics mutants are also microphthalmic, resulting from defects in cell cycle exit of proliferative retinoblasts within the developing eye. Maternal-zygotic and maternal-effect mutants demonstrate a crucial requirement for maternally derived gartand paics; these mutants show more severe developmental defects than their zygotic counterparts. Pigmentation and eye growth phenotypes in zygotic gart and paics mutants can be ascribed to separable biosynthetic pathways: pigmentation defects and microphthalmia result from deficiencies in a GTP synthesis pathway and an ATP synthesis pathway,respectively. In the absence of ATP pathway activity, S phase of proliferative retinoblasts is prolonged and cell cycle exit is compromised, which results in microphthalmia. These results demonstrate crucial maternal and zygotic requirements for de novo purine synthesis during vertebrate embryonic development, and identify independent functions for ATP and GTP pathways in mediating eye growth and pigmentation, respectively.<\/jats:p>","DOI":"10.1242\/dev.038315","type":"journal-article","created":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T20:51:20Z","timestamp":1246481480000},"page":"2601-2611","source":"Crossref","is-referenced-by-count":84,"title":["Zebrafish mutations in\n                    <i>gart<\/i>\n                    and\n                    <i>paics<\/i>\n                    identify crucial roles for de novo purine synthesis in vertebrate pigmentation and ocular development"],"prefix":"10.1242","volume":"136","author":[{"given":"Anthony","family":"Ng","sequence":"first","affiliation":[{"name":"Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722,USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa A.","family":"Uribe","sequence":"additional","affiliation":[{"name":"Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722,USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah","family":"Yieh","sequence":"additional","affiliation":[{"name":"Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722,USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Nuckels","sequence":"additional","affiliation":[{"name":"Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722,USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Gross","sequence":"additional","affiliation":[{"name":"Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722,USA."},{"name":"Institute for Neuroscience, The University of Texas at Austin, Austin, TX 78722, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042621362798500_REF1","doi-asserted-by":"crossref","unstructured":"Amsterdam, A., Nissen, R. 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