{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:04:48Z","timestamp":1792145088376,"version":"4.4.0"},"reference-count":64,"publisher":"The Company of Biologists","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,1]]},"abstract":"<jats:p>Microcephaly affects \u223c1% of the population and is associated with mental retardation, motor defects and, in some cases, seizures. We analyzed the mechanisms underlying brain size determination in a mouse model of human microcephaly. The Hertwig's anemia (an) mutant shows peripheral blood cytopenias, spontaneous aneuploidy and a predisposition to hematopoietic tumors. We found that the an mutation is a genomic inversion of exon 4 of Cdk5rap2, resulting in an in-frame deletion of exon 4 from the mRNA. The finding that CDK5RAP2 human mutations cause microcephaly prompted further analysis of Cdk5rap2an\/an mice and we demonstrated that these mice exhibit microcephaly comparable to that of the human disease, resulting from striking neurogenic defects that include proliferative and survival defects in neuronal progenitors. Cdk5rap2an\/an neuronal precursors exit the cell cycle prematurely and many undergo apoptosis. These defects are associated with impaired mitotic progression coupled with abnormal mitotic spindle pole number and mitotic orientation. Our findings suggest that the reduction in brain size observed in humans with mutations in CDK5RAP2 is associated with impaired centrosomal function and with changes in mitotic spindle orientation during progenitor proliferation.<\/jats:p>","DOI":"10.1242\/dev.040410","type":"journal-article","created":{"date-parts":[[2010,5,11]],"date-time":"2010-05-11T11:47:15Z","timestamp":1273578435000},"page":"1907-1917","source":"Crossref","is-referenced-by-count":265,"title":["Cdk5rap2 regulates centrosome function and chromosome segregation in neuronal progenitors"],"prefix":"10.1242","volume":"137","author":[{"given":"Sofia B.","family":"Lizarraga","sequence":"first","affiliation":[{"name":"Division of Genetics and the Manton Center for Orphan Disease Research, Children's Hospital Boston, Howard Hughes Medical Institute, Beth Israel-Deaconess Medical Center, and Departments of Pediatrics and Neurology, Harvard Medical School, 3 Blackfan Circle, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven P.","family":"Margossian","sequence":"additional","affiliation":[{"name":"Department of Pathology, Children's Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA."},{"name":"Division of Hematology\/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Harvard Medical School, 1 Blackfan Circle, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian H.","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Pathology, Children's Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA."},{"name":"Brigham And Women's Hospital, 45 Francis Street, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean R.","family":"Campagna","sequence":"additional","affiliation":[{"name":"Department of Pathology, Children's Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An-Ping","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Pathology, Children's Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherika","family":"Blevins","sequence":"additional","affiliation":[{"name":"Department of Pathology, Children's Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raksha","family":"Mudbhary","sequence":"additional","affiliation":[{"name":"Division of Genetics and the Manton Center for Orphan Disease Research, Children's Hospital Boston, Howard Hughes Medical Institute, Beth Israel-Deaconess Medical Center, and Departments of Pediatrics and Neurology, Harvard Medical School, 3 Blackfan Circle, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane E.","family":"Barker","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, 300 Main St, Bar Harbor, ME 04609, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher A.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Division of Genetics and the Manton Center for Orphan Disease Research, Children's Hospital Boston, Howard Hughes Medical Institute, Beth Israel-Deaconess Medical Center, and Departments of Pediatrics and Neurology, Harvard Medical School, 3 Blackfan Circle, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Fleming","sequence":"additional","affiliation":[{"name":"Department of Pathology, Children's Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042605550701400_R1","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1038\/192766b0","article-title":"Autoradiographic study of cell migration during histogenesis of cerebral cortex in the mouse","volume":"192","author":"Angevine","year":"1961","journal-title":"Nature"},{"key":"2021042605550701400_R2","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1136\/jcp.54.10.778","article-title":"High quality RNA isolation from tumours with low cellularity and high extracellular matrix component for cDNA microarrays: application to chondrosarcoma","volume":"54","author":"Baelde","year":"2001","journal-title":"J. Clin. Pathol."},{"key":"2021042605550701400_R3","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ydbio.2006.03.007","article-title":"Role of Sox2 in the development of the mouse neocortex","volume":"295","author":"Bani-Yaghoub","year":"2006","journal-title":"Dev. Biol."},{"key":"2021042605550701400_R4","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1182\/blood.V61.4.765.765","article-title":"Hertwig's anemia: characterization of the stem cell defect","volume":"61","author":"Barker","year":"1983","journal-title":"Blood"},{"key":"2021042605550701400_R5","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1016\/j.exphem.2005.06.021","article-title":"High incidence, early onset of histiocytic sarcomas in mice with Hertwig's anemia","volume":"33","author":"Barker","year":"2005","journal-title":"Exp. Hematol."},{"key":"2021042605550701400_R6","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1038\/ng995","article-title":"ASPM is a major determinant of cerebral cortical size","volume":"32","author":"Bond","year":"2002","journal-title":"Nat. Genet."},{"key":"2021042605550701400_R7","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/ng1539","article-title":"A centrosomal mechanism involving CDK5RAP2 and CENPJ controls brain size","volume":"37","author":"Bond","year":"2005","journal-title":"Nat. Genet."},{"key":"2021042605550701400_R8","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nrn2058","article-title":"Spindle regulation in neural precursors of flies and mammals","volume":"8","author":"Buchman","year":"2007","journal-title":"Nat. Rev. Neurosci."},{"key":"2021042605550701400_R9","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.neuron.2009.07.004","article-title":"Mammalian Par3 regulates progenitor cell asymmetric division via notch signaling in the developing neocortex","volume":"63","author":"Bultje","year":"2009","journal-title":"Neuron"},{"key":"2021042605550701400_R10","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1093\/cercor\/13.6.592","article-title":"Cell output, cell cycle duration and neuronal specification: a model of integrated mechanisms of the neocortical proliferative process","volume":"13","author":"Caviness","year":"2003","journal-title":"Cereb. Cortex"},{"key":"2021042605550701400_R11","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/0092-8674(95)90035-7","article-title":"Cleavage orientation and the asymmetric inheritance of Notch1 immunoreactivity in mammalian neurogenesis","volume":"82","author":"Chenn","year":"1995","journal-title":"Cell"},{"key":"2021042605550701400_R12","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1126\/science.1074192","article-title":"Regulation of cerebral cortical size by control of cell cycle exit in neural precursors","volume":"297","author":"Chenn","year":"2002","journal-title":"Science"},{"key":"2021042605550701400_R13","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/j.1469-7580.2007.00769.x","article-title":"Comparative aspects of cortical neurogenesis in vertebrates","volume":"211","author":"Cheung","year":"2007","journal-title":"J. Anat."},{"key":"2021042605550701400_R14","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0378-1119(99)00499-0","article-title":"Cloning of three novel neuronal Cdk5 activator binding proteins","volume":"242","author":"Ching","year":"2000","journal-title":"Gene"},{"key":"2021042605550701400_R15","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1523\/JNEUROSCI.2899-04.2005","article-title":"Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex","volume":"25","author":"Englund","year":"2005","journal-title":"J. Neurosci."},{"key":"2021042605550701400_R16","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1182\/blood.V64.3.727.727","article-title":"Chromosome abnormalities in mice with Hertwig's anemia","volume":"64","author":"Eppig","year":"1984","journal-title":"Blood"},{"key":"2021042605550701400_R17","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1182\/blood.V73.5.1373.1373","article-title":"Deleterious effects of irradiation and bone marrow transplantation therapy in the genetically anemic an\/an mouse","volume":"73","author":"Eppig","year":"1989","journal-title":"Blood"},{"key":"2021042605550701400_R18","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.gene.2006.02.019","article-title":"Molecular evolution of the brain size regulator genes CDK5RAP2 and CENPJ","volume":"375","author":"Evans","year":"2006","journal-title":"Gene"},{"key":"2021042605550701400_R19","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.neuron.2004.09.023","article-title":"Mitotic spindle regulation by Nde1 controls cerebral cortical size","volume":"44","author":"Feng","year":"2004","journal-title":"Neuron"},{"key":"2021042605550701400_R20","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1002\/cne.10654","article-title":"Characterization of Foxp2 and Foxp1 mRNA and protein in the developing and mature brain","volume":"460","author":"Ferland","year":"2003","journal-title":"J. Comp. Neurol."},{"key":"2021042605550701400_R21","doi-asserted-by":"crossref","first-page":"10438","DOI":"10.1073\/pnas.0604066103","article-title":"Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells","volume":"103","author":"Fish","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042605550701400_R22","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1091\/mbc.e07-04-0371","article-title":"CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the {gamma}-tubulin ring complex","volume":"19","author":"Fong","year":"2008","journal-title":"Mol. Biol. Cell"},{"key":"2021042605550701400_R23","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1038\/nrc2249","article-title":"Oncogenes and tumour suppressors take on centrosomes","volume":"7","author":"Fukasawa","year":"2007","journal-title":"Nat. Rev. Cancer"},{"key":"2021042605550701400_R24","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0896-6273(00)80778-3","article-title":"Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons","volume":"23","author":"Gleeson","year":"1999","journal-title":"Neuron"},{"key":"2021042605550701400_R25","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1038\/nrm1739","article-title":"The cell biology of neurogenesis","volume":"6","author":"Gotz","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042605550701400_R26","doi-asserted-by":"crossref","first-page":"4321","DOI":"10.1242\/jcs.020248","article-title":"Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion","volume":"120","author":"Graser","year":"2007","journal-title":"J. Cell Sci."},{"key":"2021042605550701400_R27","doi-asserted-by":"crossref","first-page":"2890","DOI":"10.1073\/pnas.0437969100","article-title":"Mitotic spindle rotation and mode of cell division in the developing telencephalon","volume":"100","author":"Haydar","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042605550701400_R28","first-page":"220","article-title":"Neue Mutationen und Koppelungsgruppen bei der Hausmaus","volume":"80","author":"Hertwig","year":"1942","journal-title":"Z. Indukt. Abstamm. Vererbungsl."},{"key":"2021042605550701400_R29","doi-asserted-by":"crossref","first-page":"3861","DOI":"10.1242\/dev.121.11.3861","article-title":"The Drosophila homeotic target gene centrosomin (cnn) encodes a novel centrosomal protein with leucine zippers and maps to a genomic region required for midgut morphogenesis","volume":"121","author":"Heuer","year":"1995","journal-title":"Development"},{"key":"2021042605550701400_R30","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0896-6273(01)00211-2","article-title":"Tbr1 regulates differentiation of the preplate and layer 6","volume":"29","author":"Hevner","year":"2001","journal-title":"Neuron"},{"key":"2021042605550701400_R31","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.tins.2007.04.001","article-title":"The centrosome in neuronal development","volume":"30","author":"Higginbotham","year":"2007","journal-title":"Trends Neurosci."},{"key":"2021042605550701400_R32","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1023\/A:1023789416385","article-title":"The Aurora kinases: role in cell transformation and tumorigenesis","volume":"22","author":"Katayama","year":"2003","journal-title":"Cancer Metastasis Rev."},{"key":"2021042605550701400_R33","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1038\/nrn2008","article-title":"Patterns of neural stem and progenitor cell division may underlie evolutionary cortical expansion","volume":"7","author":"Kriegstein","year":"2006","journal-title":"Nat. Rev. Neurosci."},{"key":"2021042605550701400_R34","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/sj.jid.5700511","article-title":"Loss of the acyl-CoA binding protein (Acbp) results in fatty acid metabolism abnormalities in mouse hair and skin","volume":"127","author":"Lee","year":"2007","journal-title":"J. Invest. Dermatol."},{"key":"2021042605550701400_R35","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.modgep.2004.03.009","article-title":"CTIP1 and CTIP2 are differentially expressed during mouse embryogenesis","volume":"4","author":"Leid","year":"2004","journal-title":"Gene Expr. Patterns"},{"issue":"Suppl. 1","key":"2021042605550701400_R36","doi-asserted-by":"crossref","first-page":"i152","DOI":"10.1093\/cercor\/bhk017","article-title":"The role of intermediate progenitor cells in the evolutionary expansion of the cerebral cortex","volume":"16","author":"Martinez-Cerdeno","year":"2006","journal-title":"Cereb. Cortex"},{"key":"2021042605550701400_R37","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1126\/science.1067132","article-title":"Transcriptional regulation of cortical neuron migration by POU domain factors","volume":"295","author":"McEvilly","year":"2002","journal-title":"Science"},{"key":"2021042605550701400_R38","doi-asserted-by":"crossref","first-page":"2829","DOI":"10.1242\/dev.126.13.2829","article-title":"The centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila","volume":"126","author":"Megraw","year":"1999","journal-title":"Development"},{"key":"2021042605550701400_R39","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0165-3806(88)90121-6","article-title":"Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker","volume":"44","author":"Misson","year":"1988","journal-title":"Brain Res. Dev. Brain Res."},{"key":"2021042605550701400_R40","first-page":"437","article-title":"Molecular genetics of lissencephaly and microcephaly","volume":"60","author":"Mochida","year":"2008","journal-title":"Brain Nerve"},{"key":"2021042605550701400_R41","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1002\/cne.20322","article-title":"Expression of Cux-1 and Cux-2 in the subventricular zone and upper layers II-IV of the cerebral cortex","volume":"479","author":"Nieto","year":"2004","journal-title":"J. Comp. Neurol."},{"key":"2021042605550701400_R42","first-page":"307","article-title":"The neocortex. An overview of its evolutionary development, structural organization and synaptology","volume":"190","author":"Nieuwenhuys","year":"1994","journal-title":"Anat. Embryol. (Berl.)"},{"key":"2021042605550701400_R43","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1038\/35055553","article-title":"Neurons derived from radial glial cells establish radial units in neocortex","volume":"409","author":"Noctor","year":"2001","journal-title":"Nature"},{"key":"2021042605550701400_R44","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1159\/000109848","article-title":"Role of intermediate progenitor cells in cerebral cortex development","volume":"30","author":"Pontious","year":"2008","journal-title":"Dev. Neurosci."},{"key":"2021042605550701400_R45","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1091\/mbc.e03-06-0405","article-title":"gamma-tubulin plays an essential role in the coordination of mitotic events","volume":"15","author":"Prigozhina","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"2021042605550701400_R46","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1126\/science.183.4123.425","article-title":"Neurons in rhesus monkey visual cortex: systematic relation between time of origin and eventual disposition","volume":"183","author":"Rakic","year":"1974","journal-title":"Science"},{"key":"2021042605550701400_R47","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1038\/nrn2719","article-title":"Evolution of the neocortex: a perspective from developmental biology","volume":"10","author":"Rakic","year":"2009","journal-title":"Nat. Rev. Neurosci."},{"key":"2021042605550701400_R48","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/0012-1606(85)90092-2","article-title":"Gametic and pleiotropic defects in mouse fetuses with Hertwig's macrocytic anemia","volume":"110","author":"Russell","year":"1985","journal-title":"Dev. Biol."},{"key":"2021042605550701400_R49","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.cell.2005.05.009","article-title":"G protein betagamma subunits and AGS3 control spindle orientation and asymmetric cell fate of cerebral cortical progenitors","volume":"122","author":"Sanada","year":"2005","journal-title":"Cell"},{"key":"2021042605550701400_R50","doi-asserted-by":"crossref","first-page":"e1000111","DOI":"10.1371\/journal.pgen.1000111","article-title":"Identification of neural outgrowth genes using genome-wide RNAi","volume":"4","author":"Sepp","year":"2008","journal-title":"PLoS Genet."},{"key":"2021042605550701400_R51","doi-asserted-by":"crossref","first-page":"9212","DOI":"10.1523\/JNEUROSCI.17-23-09212.1997","article-title":"Glutamate transporter GLAST is expressed in the radial glia-astrocyte lineage of developing mouse spinal cord","volume":"17","author":"Shibata","year":"1997","journal-title":"J. Neurosci."},{"key":"2021042605550701400_R52","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1098\/rsbl.2008.0439","article-title":"Telencephalon enlargement by the convergent evolution of expanded subventricular zones","volume":"5","author":"Striedter","year":"2009","journal-title":"Biol. Lett."},{"key":"2021042605550701400_R53","first-page":"60","article-title":"The Aurora kinase family in cell division and cancer","volume":"1786","author":"Vader","year":"2008","journal-title":"Biochim. Biophys. Acta"},{"key":"2021042605550701400_R54","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1016\/S0960-9822(99)80393-5","article-title":"Mutations in centrosomin reveal requirements for centrosomal function during early Drosophila embryogenesis","volume":"9","author":"Vaizel-Ohayon","year":"1999","journal-title":"Curr. Biol."},{"key":"2021042605550701400_R55","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1091\/mbc.e03-10-0728","article-title":"Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function","volume":"15","author":"Venkatram","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"2021042605550701400_R56","doi-asserted-by":"crossref","first-page":"11189","DOI":"10.1074\/jbc.M006546200","article-title":"Myomegalin is a novel protein of the golgi\/centrosome that interacts with a cyclic nucleotide phosphodiesterase","volume":"276","author":"Verde","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2021042605550701400_R57","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1038\/nature08435","article-title":"Asymmetric centrosome inheritance maintains neural progenitors in the neocortex","volume":"461","author":"Wang","year":"2009","journal-title":"Nature"},{"key":"2021042605550701400_R58","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1086\/429930","article-title":"Autosomal recessive primary microcephaly (MCPH): a review of clinical, molecular, and evolutionary findings","volume":"76","author":"Woods","year":"2005","journal-title":"Am. J. Hum. Genet."},{"key":"2021042605550701400_R59","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1126\/science.1134910","article-title":"Asymmetric inheritance of mother versus daughter centrosome in stem cell division","volume":"315","author":"Yamashita","year":"2007","journal-title":"Science"},{"key":"2021042605550701400_R60","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.cell.2008.01.026","article-title":"Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division","volume":"132","author":"Yingling","year":"2008","journal-title":"Cell"},{"key":"2021042605550701400_R61","doi-asserted-by":"crossref","first-page":"4037","DOI":"10.1091\/mbc.e07-05-0474","article-title":"Proper recruitment of gamma-tubulin and D-TACC\/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1","volume":"18","author":"Zhang","year":"2007","journal-title":"Mol. Biol. Cell"},{"key":"2021042605550701400_R62","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/S0076-6879(98)98021-1","article-title":"Purification and assay of gamma tubulin ring complex","volume":"298","author":"Zheng","year":"1998","journal-title":"Methods Enzymol."},{"key":"2021042605550701400_R63","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.conb.2008.05.002","article-title":"Neurogenesis and asymmetric cell division","volume":"18","author":"Zhong","year":"2008","journal-title":"Curr. Opin. Neurobiol."},{"key":"2021042605550701400_R64","doi-asserted-by":"crossref","first-page":"3642","DOI":"10.1091\/mbc.e03-11-0796","article-title":"Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entry","volume":"15","author":"Zimmerman","year":"2004","journal-title":"Mol. Biol. Cell"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/137\/11\/1907\/1570095\/1907.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/137\/11\/1907\/1570095\/1907.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T07:26:06Z","timestamp":1619421966000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/137\/11\/1907\/43793\/Cdk5rap2-regulates-centrosome-function-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,1]]},"references-count":64,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,6,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.040410","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2010,6,1]]}}}