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We found that expression of Sox10 can be maintained using noggin, Wnt3a, Lif and endothelin (NWLE). ES cell-derived Sox10-GFP-positive cells cultured in NWLE exhibit molecular markers of neural crest progenitors. They differentiate into peripheral neurons in vitro and are able to colonise the enteric network in organotypic gut cultures. Neural crest cells purified from embryos using the Sox10 reporter also survive in NWLE, but progressively succumb to differentiation. We therefore applied selection to eliminate differentiating cells. Sox10-selected cells could be clonally expanded, cryopreserved, and multiplied for over 50 days in adherent culture. They remained neurogenic in vitro and in foetal gut grafts. Generation of neural crest from mouse ES cells opens a new route to the identification and validation of determination factors. Furthermore, the ability to propagate undifferentiated progenitors creates an opportunity for experimental dissection of the stimuli and molecular circu that govern neural crest lineage progression. Finally, the demonstration of robust enteric neurogenesis provides a system for investigating and modelling cell therapeutic approaches to neurocristopathies such as Hirschsprung's disease.<\/jats:p>","DOI":"10.1242\/dev.046896","type":"journal-article","created":{"date-parts":[[2010,2,10]],"date-time":"2010-02-10T08:12:42Z","timestamp":1265789562000},"page":"693-704","source":"Crossref","is-referenced-by-count":72,"title":["Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos"],"prefix":"10.1242","volume":"137","author":[{"given":"Jitsutaro","family":"Kawaguchi","sequence":"first","affiliation":[{"name":"Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, CB2 1QR Cambridge, UK."},{"name":"Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QR Cambridge, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Nichols","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, CB2 1QR Cambridge, UK."},{"name":"Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, CB2 1QR Cambridge, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias S.","family":"Gierl","sequence":"additional","affiliation":[{"name":"Max-Delbruck-Centrum for Molecular Medicine, Robert-Rossle-Strasse 10, 13125 Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago","family":"Faial","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, CB2 1QR Cambridge, UK."},{"name":"Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QR Cambridge, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin","family":"Smith","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, CB2 1QR Cambridge, UK."},{"name":"Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QR Cambridge, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042606114191200_R1","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1038\/nbt763","article-title":"Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation","volume":"20","author":"Aubert","year":"2002","journal-title":"Nat. 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