{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T17:13:58Z","timestamp":1792257238968,"version":"4.4.0"},"reference-count":26,"publisher":"The Company of Biologists","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,1]]},"abstract":"<jats:p>The floor plate is a key organizer that controls the specification of neurons in the central nervous system. Here, we show a new role of the floor plate: segmental pattern formation of the vertebral column. Analysis of a spontaneous medaka mutant, fused centrum (fsc), which exhibits fused centra and the absence of the intervertebral ligaments, revealed that fsc encodes wnt4b, which was expressed exclusively in the floor plate. In fsc mutants, we found that wnt4b expression was completely lost in the floor plate and that abnormal conversion of the intervertebral ligament cells into osteoblasts appeared to cause a defect of the intervertebral ligaments. The establishment of the transgenic rescue lines and mosaic analyses allowed the conclusion to be drawn that production of wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column. Our findings provide a novel perspective on the mechanism of vertebrate development.<\/jats:p>","DOI":"10.1242\/dev.051540","type":"journal-article","created":{"date-parts":[[2010,5,11]],"date-time":"2010-05-11T11:47:15Z","timestamp":1273578435000},"page":"1807-1813","source":"Crossref","is-referenced-by-count":37,"title":["Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka"],"prefix":"10.1242","volume":"137","author":[{"given":"Keiji","family":"Inohaya","sequence":"first","affiliation":[{"name":"Department of Biological Information, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiro","family":"Takano","sequence":"additional","affiliation":[{"name":"Department of Hard Tissue Engineering, Tokyo Medical and Dental University, Tokyo 113-8549, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Kudo","sequence":"additional","affiliation":[{"name":"Department of Biological Information, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042605544646100_R1","doi-asserted-by":"crossref","first-page":"D747","DOI":"10.1093\/nar\/gkm765","article-title":"UTGB\/medaka: genomic resource database for medaka biology","volume":"36","author":"Ahsan","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"2021042605544646100_R2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/genetics\/15.1.1","article-title":"Further genetical studies of Aplocheilus latipes","volume":"15","author":"Aida","year":"1930","journal-title":"Genetics"},{"key":"2021042605544646100_R3","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0925-4773(00)00481-0","article-title":"The developmental fate of the rostral\/caudal half of a somite for vertebra and rib formation: experimental confirmation of the resegmentation theory using chick-quail chimeras","volume":"99","author":"Aoyama","year":"2000","journal-title":"Mech. 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