{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:11:04Z","timestamp":1790784664346,"version":"4.1.0"},"reference-count":45,"publisher":"The Company of Biologists","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,1,15]]},"abstract":"<jats:p>The pharyngeal apparatus is a transient structure that gives rise to the thymus and the parathyroid glands and also contributes to the development of arteries and the cardiac outflow tract. A typical developmental disorder of the pharyngeal apparatus is the 22q11 deletion syndrome (22q11DS), for which Tbx1 is responsible. Here, we show that Ripply3 can modulate Tbx1 activity and plays a role in the development of the pharyngeal apparatus. Ripply3 expression is observed in the pharyngeal ectoderm and endoderm and overlaps with strong expression of Tbx1 in the caudal pharyngeal endoderm. Ripply3 suppresses transcriptional activation by Tbx1 in luciferase assays in vitro. Ripply3-deficient mice exhibit abnormal development of pharyngeal derivatives, including ectopic formation of the thymus and the parathyroid gland, as well as cardiovascular malformation. Corresponding with these defects, Ripply3-deficient embryos show hypotrophy of the caudal pharyngeal apparatus. Ripply3 represses Tbx1-induced expression of Pax9 in luciferase assays in vitro, and Ripply3-deficient embryos exhibit upregulated Pax9 expression. Together, our results show that Ripply3 plays a role in pharyngeal development, probably by regulating Tbx1 activity.<\/jats:p>","DOI":"10.1242\/dev.054056","type":"journal-article","created":{"date-parts":[[2010,12,21]],"date-time":"2010-12-21T07:33:04Z","timestamp":1292916784000},"page":"339-348","source":"Crossref","is-referenced-by-count":66,"title":["Ripply3, a Tbx1 repressor, is required for development of the pharyngeal apparatus and its derivatives in mice"],"prefix":"10.1242","volume":"138","author":[{"given":"Tadashi","family":"Okubo","sequence":"first","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan."},{"name":"Department of Basic Biology, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi 444-8787, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akinori","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan."},{"name":"Department of Basic Biology, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi 444-8787, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisato","family":"Yagi","sequence":"additional","affiliation":[{"name":"International Research and Educational Institute for Integrated Medical Sciences, Tokyo Women's Medical University, Shinjuku-ku, Tokyo 162-8666, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masae","family":"Morishima","sequence":"additional","affiliation":[{"name":"International Research and Educational Institute for Integrated Medical Sciences, Tokyo Women's Medical University, Shinjuku-ku, Tokyo 162-8666, Japan."},{"name":"Department of Pediatric Cardiology, Tokyo Woman's Medical University, Shinjuku-ku, Tokyo 162-8666, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumiko","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"International Research and Educational Institute for Integrated Medical Sciences, Tokyo Women's Medical University, Shinjuku-ku, Tokyo 162-8666, Japan."},{"name":"Department of Pediatric Cardiology, Tokyo Woman's Medical University, Shinjuku-ku, Tokyo 162-8666, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Takada","sequence":"additional","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan."},{"name":"Department of Basic Biology, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi 444-8787, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042607114335100_R1","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1093\/hmg\/ddl084","article-title":"Tissue-specific roles of Tbx1 in the development of the outer, middle and inner ear, defective in 22q11DS patients","volume":"15","author":"Arnold","year":"2006","journal-title":"Hum. 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