{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:08:39Z","timestamp":1791050919141,"version":"4.1.0"},"reference-count":56,"publisher":"The Company of Biologists","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,6,15]]},"abstract":"<jats:p>The infundibulum links the nervous and endocrine systems, serving as a crucial integrating centre for body homeostasis. Here we describe that the chick infundibulum derives from two subsets of anterior ventral midline cells. One set remains at the ventral midline and forms the posterior-ventral infundibulum. A second set migrates laterally, forming a collar around the midline. We show that collar cells are composed of Fgf3+ SOX3+ proliferating progenitors, the induction of which is SHH dependent, but the maintenance of which requires FGF signalling. Collar cells proliferate late into embryogenesis, can generate neurospheres that passage extensively, and differentiate to distinct fates, including hypothalamic neuronal fates and Fgf10+ anterior-dorsal infundibular cells. Together, our study shows that a subset of anterior floor plate-like cells gives rise to Fgf3+ SOX3+ progenitor cells, demonstrates a dual origin of infundibular cells and reveals a crucial role for FGF signalling in governing extended infundibular growth.<\/jats:p>","DOI":"10.1242\/dev.062794","type":"journal-article","created":{"date-parts":[[2011,5,24]],"date-time":"2011-05-24T11:32:58Z","timestamp":1306236778000},"page":"2613-2624","source":"Crossref","is-referenced-by-count":44,"title":["FGF-dependent midline-derived progenitor cells in hypothalamic infundibular development"],"prefix":"10.1242","volume":"138","author":[{"given":"Caroline Alayne","family":"Pearson","sequence":"first","affiliation":[{"name":"MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoji","family":"Ohyama","sequence":"additional","affiliation":[{"name":"MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liz","family":"Manning","sequence":"additional","affiliation":[{"name":"MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soheil","family":"Aghamohammadzadeh","sequence":"additional","affiliation":[{"name":"MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Sang","sequence":"additional","affiliation":[{"name":"The Roslin Institute, University of Edinburgh, Roslin EH25 9PS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marysia","family":"Placzek","sequence":"additional","affiliation":[{"name":"MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042606562359200_R1","doi-asserted-by":"crossref","first-page":"2877","DOI":"10.1101\/gad.357705","article-title":"FGF-dependent Notch signaling maintains the spinal cord stem zone","volume":"19","author":"Akai","year":"2005","journal-title":"Genes Dev."},{"key":"2021042606562359200_R2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.cell.2005.10.037","article-title":"Identification of intrinsic determinants of midbrain dopamine neurons","volume":"124","author":"Andersson","year":"2006","journal-title":"Cell"},{"key":"2021042606562359200_R3","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1006\/dbio.1996.0337","article-title":"AltFGF-2, a novel ER-associated FGF-2 protein isoform: its embryonic distribution and functional analysis during neural tube development","volume":"180","author":"Borja","year":"1996","journal-title":"Dev. Biol."},{"key":"2021042606562359200_R4","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1038\/nn1131","article-title":"Vertebrate neurogenesis is counteracted by Sox1-3 activity","volume":"6","author":"Bylund","year":"2003","journal-title":"Nat. Neurosci."},{"key":"2021042606562359200_R5","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1002\/neu.20169","article-title":"Morphogens in motion: growth control of the neural tube","volume":"64","author":"Cayuso","year":"2005","journal-title":"J. Neurobiol."},{"key":"2021042606562359200_R6","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1146\/annurev.ne.18.030195.002433","article-title":"The role of the floor plate in axon guidance","volume":"18","author":"Colamarino","year":"1995","journal-title":"Annu. Rev. Neurosci."},{"key":"2021042606562359200_R7","doi-asserted-by":"crossref","first-page":"2557","DOI":"10.1210\/jc.2004-0428","article-title":"Proopiomelanocortin and energy balance: insights from human and murine genetics","volume":"89","author":"Coll","year":"2004","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"2021042606562359200_R8","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.1101\/gad.932601","article-title":"Temporal regulation of a paired-like homeodomain repressor\/TLE corepressor complex and a related activator is required for pituitary organogenesis","volume":"15","author":"Dasen","year":"2001","journal-title":"Genes Dev."},{"key":"2021042606562359200_R9","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/978-1-60327-931-4_7","article-title":"Isolation, expansion, and differentiation of adult Mammalian neural stem and progenitor cells using the neurosphere assay","volume":"549","author":"Deleyrolle","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"2021042606562359200_R10","doi-asserted-by":"crossref","first-page":"4273","DOI":"10.1242\/dev.02009","article-title":"Specification and maintenance of the spinal cord stem zone","volume":"132","author":"Delfino-Mach\u00edn","year":"2005","journal-title":"Development"},{"key":"2021042606562359200_R11","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1159\/000207479","article-title":"Developmental abnormalities of the posterior pituitary gland","volume":"14","author":"di Iorgi","year":"2009","journal-title":"Endocr. Dev."},{"key":"2021042606562359200_R12","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0896-6273(03)00565-8","article-title":"Opposing FGF and retinoid pathways control ventral neural pattern, neuronal differentiation, and segmentation during body axis extension","volume":"40","author":"Diez del Corral","year":"2003","journal-title":"Neuron"},{"key":"2021042606562359200_R13","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1159\/000082134","article-title":"SOX2, a persistent marker for multipotential neural stem cells derived from embryonic stem cells, the embryo or the adult","volume":"26","author":"Ellis","year":"2004","journal-title":"Dev. Neurosci."},{"key":"2021042606562359200_R14","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1101\/SQB.1997.062.01.053","article-title":"Graded sonic hedgehog signaling and the specification of cell fate in the ventral neural tube","volume":"62","author":"Ericson","year":"1997","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"2021042606562359200_R15","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1242\/dev.125.6.1005","article-title":"Integrated FGF and BMP signaling controls the progression of progenitor cell differentiation and the emergence of pattern in the embryonic anterior pituitary","volume":"125","author":"Ericson","year":"1998","journal-title":"Development"},{"key":"2021042606562359200_R16","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/S0896-6273(03)00497-5","article-title":"SOX2 functions to maintain neural progenitor identity","volume":"39","author":"Graham","year":"2003","journal-title":"Neuron"},{"key":"2021042606562359200_R17","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1242\/dev.129.6.1533","article-title":"FGF10 maintains stem cell compartment in developing mouse incisors","volume":"129","author":"Harada","year":"2002","journal-title":"Development"},{"key":"2021042606562359200_R18","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/S0012-1606(03)00218-5","article-title":"A novel inducible element, activated by contact with Rathke's pouch, is present in the regulatory region of the Rpx\/Hesx1 homeobox gene","volume":"260","author":"Hermesz","year":"2003","journal-title":"Dev. Biol."},{"key":"2021042606562359200_R19","doi-asserted-by":"crossref","first-page":"3681","DOI":"10.1242\/dev.01235","article-title":"Fgf3 signaling from the ventral diencephalon is required for early specification and subsequent survival of the zebrafish adenohypophysis","volume":"131","author":"Herzog","year":"2004","journal-title":"Development"},{"key":"2021042606562359200_R20","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0896-6273(95)90062-4","article-title":"Induction of midbrain dopaminergic neurons by Sonic hedgehog","volume":"15","author":"Hynes","year":"1995","journal-title":"Neuron"},{"key":"2021042606562359200_R21","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.1242\/dev.031344","article-title":"Morphogenesis and cytodifferentiation of the avian retinal pigmented epithelium require downregulation of Group B1 Sox genes","volume":"136","author":"Ishii","year":"2009","journal-title":"Development"},{"key":"2021042606562359200_R22","first-page":"87","article-title":"Floor plate-derived signals and the control of neural cell pattern in vertebrates","volume":"86","author":"Jessell","year":"1990","journal-title":"Harvey Lect."},{"key":"2021042606562359200_R23","doi-asserted-by":"crossref","first-page":"5923","DOI":"10.1242\/dev.01453","article-title":"Inhibition of Wnt\/Axin\/{beta}-catenin pathway activity promotes ventral CNS midline tissue to adopt hypothalamic rather than floor plate identity","volume":"131","author":"Kapsimali","year":"2004","journal-title":"Development"},{"key":"2021042606562359200_R24","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1242\/dev.129.1.83","article-title":"Early subdivisions in the neural plate define distinct competence for inductive signals","volume":"129","author":"Kobayashi","year":"2002","journal-title":"Development"},{"key":"2021042606562359200_R25","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.devcel.2006.09.021","article-title":"Regional morphogenesis in the hypothalamus: a BMP-Tbx2 pathway coordinates fate and proliferation through Shh downregulation","volume":"11","author":"Manning","year":"2006","journal-title":"Dev. Cell"},{"key":"2021042606562359200_R26","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/35078535","article-title":"FGF receptor signalling is required to maintain neural progenitors during Hensen's node progression","volume":"3","author":"Mathis","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"2021042606562359200_R27","doi-asserted-by":"crossref","first-page":"2289","DOI":"10.1242\/dev.022020","article-title":"Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity","volume":"135","author":"McGrew","year":"2008","journal-title":"Development"},{"key":"2021042606562359200_R28","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1038\/nature02060","article-title":"Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation","volume":"425","author":"Molofsky","year":"2003","journal-title":"Nature"},{"key":"2021042606562359200_R29","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0925-4773(00)00393-2","article-title":"Fibroblast growth factor signaling is required for the proliferation and patterning of progenitor cells in the developing anterior pituitary","volume":"96","author":"Norlin","year":"2000","journal-title":"Mech. Dev."},{"key":"2021042606562359200_R30","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1006\/bbrc.2000.3721","article-title":"FGF10 acts as a major ligand for FGF receptor 2 IIIb in mouse multi-organ development","volume":"277","author":"Ohuchi","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021042606562359200_R31","doi-asserted-by":"crossref","first-page":"5185","DOI":"10.1242\/dev.02094","article-title":"Directed differentiation of neural cells to hypothalamic dopaminergic neurons","volume":"132","author":"Ohyama","year":"2005","journal-title":"Development"},{"key":"2021042606562359200_R32","doi-asserted-by":"crossref","first-page":"3325","DOI":"10.1242\/dev.027078","article-title":"Temporal progression of hypothalamic patterning by a dual action of BMP","volume":"135","author":"Ohyama","year":"2008","journal-title":"Development"},{"key":"2021042606562359200_R33","doi-asserted-by":"crossref","first-page":"3213","DOI":"10.1242\/dev.02879","article-title":"Differences in neurogenic potential in floor plate cells along an anteroposterior location: midbrain dopaminergic neurons originate from mesencephalic floor plate cells","volume":"134","author":"Ono","year":"2007","journal-title":"Development"},{"key":"2021042606562359200_R34","first-page":"1","article-title":"Anatomy of the hypothalamic-pituitary axis","volume":"14","author":"Pelletier","year":"1991","journal-title":"Methods Achiev. Exp. Pathol."},{"key":"2021042606562359200_R35","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.conb.2005.01.016","article-title":"SOX genes and neural progenitor identity","volume":"15","author":"Pevny","year":"2005","journal-title":"Curr. Opin. Neurobiol."},{"key":"2021042606562359200_R36","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1242\/dev.125.10.1967","article-title":"A role for SOX1 in neural determination","volume":"125","author":"Pevny","year":"1998","journal-title":"Development"},{"key":"2021042606562359200_R37","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/nrn1628","article-title":"The floor plate; multiple cells, multiple signals","volume":"6","author":"Placzek","year":"2005","journal-title":"Nat. Rev. Neurosci."},{"key":"2021042606562359200_R38","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1242\/dev.117.1.205","article-title":"Induction of floor plate differentiation by contact-dependent, homeogenetic signals","volume":"117","author":"Placzek","year":"1993","journal-title":"Development"},{"key":"2021042606562359200_R39","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.gep.2008.04.006","article-title":"Expression of Tbx2 and Tbx3 in the developing hypothalamic-pituitary axis","volume":"8","author":"Pontecorvi","year":"2008","journal-title":"Gene Expr. Patterns"},{"key":"2021042606562359200_R40","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1002\/dvdy.20028","article-title":"Regulatory analysis of the mouse Fgf3 gene: control of embryonic expression patterns and dependence upon sonic hedgehog (Shh) signalling","volume":"230","author":"Powles","year":"2004","journal-title":"Dev. Dyn."},{"key":"2021042606562359200_R41","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/ng1309","article-title":"SOX3 is required during the formation of the hypothalamo-pituitary axis","volume":"36","author":"Rizzoti","year":"2004","journal-title":"Nat. Genet."},{"key":"2021042606562359200_R42","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.ydbio.2007.10.023","article-title":"Sox3 expression is maintained by FGF signaling and restricted to the neural plate by Vent proteins in the Xenopus embryo","volume":"313","author":"Rogers","year":"2008","journal-title":"Dev. Biol."},{"key":"2021042606562359200_R43","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1038\/nn.2646","article-title":"SOX9 induces and maintains neural stem cells","volume":"13","author":"Scott","year":"2010","journal-title":"Nat. Neurosci."},{"key":"2021042606562359200_R44","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1242\/dev.02244","article-title":"Belladonna\/(Ihx2) is required for neural patterning and midline axon guidance in the zebrafish forebrain","volume":"133","author":"Seth","year":"2006","journal-title":"Development"},{"key":"2021042606562359200_R45","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1242\/dev.029157","article-title":"FGF3 in the floor plate directs notochord convergent extension in the Ciona tadpole","volume":"136","author":"Shi","year":"2009","journal-title":"Development"},{"key":"2021042606562359200_R46","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1242\/dev.02858","article-title":"A discrete period of FGF-induced Erk1\/2 signalling is required for vertebrate neural specification","volume":"134","author":"Stavridis","year":"2007","journal-title":"Development"},{"key":"2021042606562359200_R47","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/35017617","article-title":"Initiation of neural induction by FGF signalling before gastrulation","volume":"406","author":"Streit","year":"2000","journal-title":"Nature"},{"key":"2021042606562359200_R48","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1242\/dev.02196","article-title":"Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1","volume":"133","author":"Takemoto","year":"2005","journal-title":"Development"},{"key":"2021042606562359200_R49","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1242\/dev.115.3.695","article-title":"Developmental expression of the Xenopus int-2 (FGF-3) gene: activation by mesodermal and neural induction","volume":"115","author":"Tannahill","year":"1992","journal-title":"Development"},{"key":"2021042606562359200_R50","doi-asserted-by":"crossref","first-page":"3417","DOI":"10.1002\/dvdy.21765","article-title":"Differential requirements for Fgf3 and Fgf8 during mouse forebrain development","volume":"237","author":"Theil","year":"2008","journal-title":"Dev. Dyn."},{"key":"2021042606562359200_R51","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.yfrne.2010.11.002","article-title":"Fibroblast growth factor signalling in the developing neuroendocrine hypothalamus","volume":"32","author":"Tsai","year":"2011","journal-title":"Front. Neuroendocrinol."},{"key":"2021042606562359200_R52","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1242\/dev.049718","article-title":"Proliferative and transcriptional identity of distinct classes of neural precursors in the mammalian olfactory epithelium","volume":"137","author":"Tucker","year":"2010","journal-title":"Development"},{"key":"2021042606562359200_R53","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S0960-9822(00)00431-0","article-title":"An early requirement for FGF signalling in the acquisition of neural cell fate in the chick embryo","volume":"10","author":"Wilson","year":"2000","journal-title":"Curr. Biol."},{"key":"2021042606562359200_R54","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1086\/430134","article-title":"Over- and underdosage of SOX3 is associated with infundibular hypoplasia and hypopituitarism","volume":"76","author":"Woods","year":"2005","journal-title":"Am. J. Hum. Genet."},{"key":"2021042606562359200_R55","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.ydbio.2009.11.002","article-title":"A role of the LIM-homeobox gene Lhx2 in the regulation of pituitary development","volume":"337","author":"Zhao","year":"2010","journal-title":"Dev. Biol."},{"key":"2021042606562359200_R56","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1152\/physrev.00006.2006","article-title":"Molecular physiology of pituitary development: signaling and transcriptional networks","volume":"87","author":"Zhu","year":"2007","journal-title":"Physiol. Rev."}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/138\/12\/2613\/1196002\/2613.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/138\/12\/2613\/1196002\/2613.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T09:39:47Z","timestamp":1619429987000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/138\/12\/2613\/44472\/FGF-dependent-midline-derived-progenitor-cells-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6,15]]},"references-count":56,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2011,6,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.062794","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2011,6,15]]}}}