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Unexpectedly, we found small micromeres accumulate 2.32 times more of the ABC transporter substrates calcein-AM, CellTrace RedOrange, BoDipy-verapamil and BoDipy-vinblastine, than any other cell in the embryo, indicating a reduction in multidrug efflux activity. The reduction in small micromere ABC transporter activity is mediated by a pulse of endocytosis occurring 20-60 minutes after the appearance of the micromeres \u2013 the precursors of the small micromeres. Treating embryos with phenylarsine oxide, an inhibitor of endocytosis, prevents the reduction of transporter activity. Tetramethylrhodamine dextran and cholera toxin B uptake experiments indicate that micromeres have higher rates of bulk and raft-associated membrane endocytosis during the window of transporter downregulation. We hypothesized that this loss of efflux transport could be required for the detection of developmental signaling molecules such as germ cell chemoattractants. Consistent with this hypothesis, we found that the inhibition of ABCB and ABCC-types of efflux transporters disrupts the ordered distribution of small micromeres to the left and right coelomic pouches. These results point to tradeoffs between signaling and the protective functions of the transporters.<\/jats:p>","DOI":"10.1242\/dev.076752","type":"journal-article","created":{"date-parts":[[2012,1,24]],"date-time":"2012-01-24T10:05:19Z","timestamp":1327399519000},"page":"783-792","source":"Crossref","is-referenced-by-count":31,"title":["Programmed reduction of ABC transporter activity in sea urchin germline progenitors"],"prefix":"10.1242","volume":"139","author":[{"given":"Joseph P.","family":"Campanale","sequence":"first","affiliation":[{"name":"Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0202, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amro","family":"Hamdoun","sequence":"additional","affiliation":[{"name":"Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0202, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042606370828700_R1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ydbio.2009.02.039","article-title":"Cell polarity emerges at first cleavage in sea urchin embryos","volume":"330","author":"Alford","year":"2009","journal-title":"Dev. 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