{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:52:12Z","timestamp":1790542332330,"version":"4.1.0"},"reference-count":52,"publisher":"The Company of Biologists","issue":"20","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,10,15]]},"abstract":"<jats:p>The transcriptional repressor Rest (Nrsf) recruits chromatin-modifying complexes to RE1 \u2018silencer elements\u2019, which are associated with hundreds of neural genes. However, the requirement for Rest-mediated transcriptional regulation of embryonic development and cell fate is poorly understood. Conflicting views of the role of Rest in controlling cell fate have emerged from recent studies. To address these controversies, we examined the developmental requirement for Rest in zebrafish using zinc-finger nuclease-mediated gene targeting. We discovered that germ layer specification progresses normally in rest mutants despite derepression of target genes during embryogenesis. This analysis provides the first evidence that maternal rest is essential for repression of target genes during blastula stages. Surprisingly, neurogenesis proceeds largely normally in rest mutants, although abnormalities are observed within the nervous system, including defects in oligodendrocyte precursor cell development and a partial loss of facial branchiomotor neuron migration. Mutants progress normally through embryogenesis but many die as larvae (after 12 days). However, some homozygotes reach adulthood and are viable. We utilized an RE1\/NRSE transgenic reporter system to dynamically monitor Rest activity. This analysis revealed that Rest is required to repress gene expression in mesodermal derivatives including muscle and notochord, as well as within the nervous system. Finally, we demonstrated that Rest is required for long-term repression of target genes in non-neural tissues in adult zebrafish. Our results point to a broad role for Rest in fine-tuning neural gene expression, rather than as a widespread regulator of neurogenesis or cell fate.<\/jats:p>","DOI":"10.1242\/dev.080994","type":"journal-article","created":{"date-parts":[[2012,9,6]],"date-time":"2012-09-06T01:58:04Z","timestamp":1346896684000},"page":"3838-3848","source":"Crossref","is-referenced-by-count":30,"title":["Zebrafish <i>rest<\/i> regulates developmental gene expression but not neurogenesis"],"prefix":"10.1242","volume":"139","author":[{"given":"Fatma O.","family":"Kok","sequence":"first","affiliation":[{"name":"Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY 11794, USA"},{"name":"Graduate Program in Molecular and Cellular Biology, Stony Brook University, Stony Brook, NY 11794, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Taibi","sequence":"additional","affiliation":[{"name":"Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY 11794, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J.","family":"Wanner","sequence":"additional","affiliation":[{"name":"Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiayang","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Georgia Health Sciences University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cara E.","family":"Moravec","sequence":"additional","affiliation":[{"name":"Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY 11794, USA"},{"name":"Genetics Graduate Program, Stony Brook University, Stony Brook, NY 11794, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crystal E.","family":"Love","sequence":"additional","affiliation":[{"name":"Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL 60637, USA"},{"name":"Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria E.","family":"Prince","sequence":"additional","affiliation":[{"name":"Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL 60637, USA"},{"name":"Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff S.","family":"Mumm","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Georgia Health Sciences University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard I.","family":"Sirotkin","sequence":"additional","affiliation":[{"name":"Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY 11794, USA"},{"name":"Graduate Program in Molecular and Cellular Biology, Stony Brook University, Stony Brook, NY 11794, USA"},{"name":"Genetics Graduate Program, Stony Brook University, Stony Brook, NY 11794, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042606303944900_R1","doi-asserted-by":"crossref","first-page":"e7665","DOI":"10.1371\/journal.pone.0007665","article-title":"Differential deployment of REST and CoREST promotes glial subtype specification and oligodendrocyte lineage maturation","volume":"4","author":"Abrajano","year":"2009","journal-title":"PLoS ONE"},{"key":"2021042606303944900_R2","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1242\/dev.072272","article-title":"Genetic ablation of Rest leads to in vitro-specific derepression of neuronal genes during neurogenesis","volume":"139","author":"Aoki","year":"2012","journal-title":"Development"},{"key":"2021042606303944900_R3","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0896-6273(01)00371-3","article-title":"Regulation of neuronal traits by a novel transcriptional complex","volume":"31","author":"Ballas","year":"2001","journal-title":"Neuron"},{"key":"2021042606303944900_R4","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.cell.2005.03.013","article-title":"REST and its corepressors mediate plasticity of neuronal gene chromatin throughout neurogenesis","volume":"121","author":"Ballas","year":"2005","journal-title":"Cell"},{"key":"2021042606303944900_R5","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1074\/jbc.M310353200","article-title":"Distinct RE-1 silencing transcription factor-containing complexes interact with different target genes","volume":"279","author":"Belyaev","year":"2004","journal-title":"J. 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