{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T22:19:54Z","timestamp":1789942794802,"version":"4.0.1"},"reference-count":66,"publisher":"The Company of Biologists","issue":"23","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,12,1]]},"abstract":"<jats:p>The Notch signaling pathway is an important contributor to the development and homeostasis of the cardiovascular system. Not surprisingly, mutations in Notch receptors and ligands have been linked to a variety of hereditary diseases that impact both the heart and the vasculature. In particular, mutations in the gene encoding the human Notch ligand jagged 1 result in a multisystem autosomal dominant disorder called Alagille syndrome, which includes tetralogy of Fallot among its more severe cardiac pathologies. Jagged 1 is expressed throughout the developing embryo, particularly in endothelial cells. Here, we demonstrate that endothelial-specific deletion of Jag1 leads to cardiovascular defects in both embryonic and adult mice that are reminiscent of those in Alagille syndrome. Mutant mice display right ventricular hypertrophy, overriding aorta, ventricular septal defects, coronary vessel abnormalities and valve defects. Examination of mid-gestational embryos revealed that the loss of Jag1, similar to the loss of Notch1, disrupts endothelial-to-mesenchymal transition during endocardial cushion formation. Furthermore, adult mutant mice exhibit cardiac valve calcifications associated with abnormal matrix remodeling and induction of bone morphogenesis. This work shows that the endothelium is responsible for the wide spectrum of cardiac phenotypes displayed in Alagille Syndrome and it demonstrates a crucial role for Jag1 in valve morphogenesis.<\/jats:p>","DOI":"10.1242\/dev.084871","type":"journal-article","created":{"date-parts":[[2012,10,24]],"date-time":"2012-10-24T20:29:02Z","timestamp":1351110542000},"page":"4449-4460","source":"Crossref","is-referenced-by-count":108,"title":["Endothelial deletion of murine\n                    <i>Jag1<\/i>\n                    leads to valve calcification and congenital heart defects associated with Alagille syndrome"],"prefix":"10.1242","volume":"139","author":[{"given":"Jennifer J.","family":"Hofmann","sequence":"first","affiliation":[{"name":"Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anais","family":"Briot","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Enciso","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Department of Pediatrics, University of California, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann C.","family":"Zovein","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, CA 90095, USA"},{"name":"Division of Neonatology, Department of Pediatrics, University of California, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxun","family":"Ren","sequence":"additional","affiliation":[{"name":"Division of Anesthesiology, Department of Medicine, University of California, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen W.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520-8017, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freddy","family":"Radtke","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne, Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Simons","sequence":"additional","affiliation":[{"name":"Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520-8017, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibin","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Anesthesiology, Department of Medicine, University of California, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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Dyn."},{"key":"2021042606331683700_R3","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1161\/01.RES.0000141146.95728.da","article-title":"Heart valve development: endothelial cell signaling and differentiation","volume":"95","author":"Armstrong","year":"2004","journal-title":"Circ. Res."},{"key":"2021042606331683700_R4","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1002\/humu.21231","article-title":"Jagged1 (JAG1) mutations in patients with tetralogy of Fallot or pulmonic stenosis","volume":"31","author":"Bauer","year":"2010","journal-title":"Hum. Mutat."},{"key":"2021042606331683700_R5","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1016\/j.cell.2009.03.025","article-title":"The notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis","volume":"137","author":"Benedito","year":"2009","journal-title":"Cell"},{"key":"2021042606331683700_R6","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1006\/dbio.2002.0731","article-title":"Temporal and distinct TGFbeta ligand requirements during mouse and avian endocardial cushion morphogenesis","volume":"248","author":"Camenisch","year":"2002","journal-title":"Dev. Biol."},{"key":"2021042606331683700_R7","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1083\/jcb.200906044","article-title":"Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells","volume":"188","author":"Chen","year":"2010","journal-title":"J. Cell Biol."},{"key":"2021042606331683700_R8","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1053\/jhep.2000.16600","article-title":"JAGGED1 gene expression during human embryogenesis elucidates the wide phenotypic spectrum of Alagille syndrome","volume":"32","author":"Crosnier","year":"2000","journal-title":"Hepatology"},{"key":"2021042606331683700_R9","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.devcel.2012.01.014","article-title":"Coordinating tissue interactions: Notch signaling in cardiac development and disease","volume":"22","author":"de la Pompa","year":"2012","journal-title":"Dev. Cell"},{"key":"2021042606331683700_R10","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/ni1164","article-title":"Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance","volume":"6","author":"Duncan","year":"2005","journal-title":"Nat. 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Cardiol."},{"key":"2021042606331683700_R41","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1161\/01.RES.0000124300.76171.C9","article-title":"Notch activation results in phenotypic and functional changes consistent with endothelial-to-mesenchymal transformation","volume":"94","author":"Noseda","year":"2004","journal-title":"Circ. Res."},{"key":"2021042606331683700_R42","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.1126\/science.1132292","article-title":"Gene regulatory networks in the evolution and development of the heart","volume":"313","author":"Olson","year":"2006","journal-title":"Science"},{"key":"2021042606331683700_R43","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1093\/eurheartj\/ehp175","article-title":"Calcific aortic valve disease: outflow obstruction is the end stage of a systemic disease process","volume":"30","author":"Otto","year":"2009","journal-title":"Eur. Heart J."},{"key":"2021042606331683700_R44","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.semcdb.2012.01.010","article-title":"Notch signaling in human development and disease","volume":"23","author":"Penton","year":"2012","journal-title":"Semin. Cell Dev. Biol."},{"key":"2021042606331683700_R45","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0074-7696(05)43005-3","article-title":"Cell biology of cardiac cushion development","volume":"243","author":"Person","year":"2005","journal-title":"Int. Rev. Cytol."},{"key":"2021042606331683700_R46","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1002\/ajmg.10652","article-title":"Jagged1 gene mutation for abdominal coarctation of the aorta in Alagille syndrome","volume":"112","author":"Raas-Rothschild","year":"2002","journal-title":"Am. J. Med. 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