{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:57:26Z","timestamp":1790211446268,"version":"4.1.0"},"reference-count":35,"publisher":"The Company of Biologists","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,4,1]]},"abstract":"<jats:p>CLIPR-59 is a new member of the cytoplasmic linker proteins (CLIP) family mainly localized to the trans-Golgi network. We show here that Clipr-59 expression in mice is restricted to specific pools of neurons, in particular motoneurons (MNs), and progressively increases from embryonic day 12.5 (E12.5) until the first postnatal days. We generated a Clipr-59 knockout mouse model that presents perinatal lethality due to respiratory defects. Physiological experiments revealed that this altered innervation prevents the normal nerve-elicited contraction of the mutant diaphragm that is reduced both in amplitude and fatigue-resistance at E18.5, despite unaffected functional muscular contractility. Innervation of the mutant diaphragm is not altered until E15.5, but is then partially lost in the most distal parts of the muscle. Ultrastructural observations of neuromuscular junctions (NMJs) in the distal region of the diaphragm reveal a normal organization, but a lower density of nerve terminals capped by terminal Schwann cells in E18.5 mutant when compared with control embryos. Similar defects in NMJ stability, with a hierarchy of severity along the caudo-rostral axis, are also observed in other muscles innervated by facial and spinal MNs in Clipr-59 mutant mice. Clipr-59 deficiency therefore affects axon maintenance but not axon guidance toward muscle targets. Thus, CLIPR-59 is involved in the stabilization of specific motor axons at the NMJ during mouse late embryogenesis and its role is crucial for mouse perinatal development.<\/jats:p>","DOI":"10.1242\/dev.087106","type":"journal-article","created":{"date-parts":[[2013,3,12]],"date-time":"2013-03-12T11:27:44Z","timestamp":1363087664000},"page":"1583-1593","source":"Crossref","is-referenced-by-count":7,"title":["CLIPR-59: a protein essential for neuromuscular junction stability during mouse late embryonic development"],"prefix":"10.1242","volume":"140","author":[{"given":"Aur\u00e9lie","family":"Couesnon","sequence":"first","affiliation":[{"name":"Institut Cochin, Universit\u00e9 Paris Descartes, CNRS UMR 8104, Paris, France."},{"name":"Inserm, U1016, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Offner","sequence":"additional","affiliation":[{"name":"Institut Cochin, Universit\u00e9 Paris Descartes, CNRS UMR 8104, Paris, France."},{"name":"Inserm, U1016, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Bernard","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 952, CNRS UMR7224, Universit\u00e9 Pierre et Marie Curie, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Chaverot","sequence":"additional","affiliation":[{"name":"Institut Cochin, Universit\u00e9 Paris Descartes, CNRS UMR 8104, Paris, France."},{"name":"Inserm, U1016, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Backer","sequence":"additional","affiliation":[{"name":"Institut Cochin, Universit\u00e9 Paris Descartes, CNRS UMR 8104, Paris, France."},{"name":"Inserm, U1016, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Dimitrov","sequence":"additional","affiliation":[{"name":"Institut Curie, Paris, France."},{"name":"CNRS UMR144, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Perez","sequence":"additional","affiliation":[{"name":"Institut Curie, Paris, France."},{"name":"CNRS UMR144, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Molg\u00f3","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Neurobiologie Alfred Fessard - FRC2118, Laboratoire de Neurobiologie et D\u00e9veloppement UPR3294, Gif sur Yvette, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyne","family":"Bloch-Gallego","sequence":"additional","affiliation":[{"name":"Institut Cochin, Universit\u00e9 Paris Descartes, CNRS UMR 8104, Paris, France."},{"name":"Inserm, U1016, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042607404305600_R1","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1101\/gad.344505","article-title":"The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis","volume":"19","author":"Akhmanova","year":"2005","journal-title":"Genes Dev."},{"key":"2021042607404305600_R2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/(SICI)1097-4547(19990101)55:1<119::AID-JNR13>3.0.CO;2-6","article-title":"Cardiotrophin-1 requires LIFRbeta to promote survival of mouse motoneurons purified by a novel technique","volume":"55","author":"Arce","year":"1999","journal-title":"J. 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