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Genetic deletion of the laminin \u03b22 and \u03b33 chains affects astrocyte migration and spatial distribution. We show that laminins act as haptotactic factors in vitro in an isoform-specific manner, inducing astrocyte migration and promoting astrocyte differentiation. The addition of exogenous laminins to laminin-null retinal explants rescues astrocyte migration and spatial patterning. Furthermore, we show that the loss of laminins reduces \u03b21 integrin expression in astrocytes. Culturing laminin-null retinal astrocytes on laminin substrates restores focal localization of \u03b21 integrin. Finally, we show that laminins containing \u03b22 and \u03b33 chains regulate subsequent retinal blood vessel growth and maintain vascular integrity. These in vivo and in vitro studies demonstrate clearly that laminins containing \u03b22 and \u03b33 chains are indispensable for migration and spatial organization of astrocytes and that they play a crucial role during retinal angiogenesis in vivo.<\/jats:p>","DOI":"10.1242\/dev.087817","type":"journal-article","created":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T11:19:21Z","timestamp":1365506361000},"page":"2050-2060","source":"Crossref","is-referenced-by-count":99,"title":["Laminins containing the \u03b22 and \u03b33 chains regulate astrocyte migration and angiogenesis in the retina"],"prefix":"10.1242","volume":"140","author":[{"given":"Gopalan","family":"Gnanaguru","sequence":"first","affiliation":[{"name":"Departments of Ophthalmology and Cell Biology, and the SUNY Eye Institute, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Bachay","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Cell Biology, and the SUNY Eye Institute, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saptarshi","family":"Biswas","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Cell Biology, and the SUNY Eye Institute, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Germ\u00e1n","family":"Pinz\u00f3n-Duarte","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Cell Biology, and the SUNY Eye Institute, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale D.","family":"Hunter","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Cell Biology, and the SUNY Eye Institute, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Brunken","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Cell Biology, and the SUNY Eye Institute, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042607363906800_R1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/nrn1824","article-title":"Astrocyte-endothelial interactions at the blood-brain barrier","volume":"7","author":"Abbott","year":"2006","journal-title":"Nat. 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