{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:29:03Z","timestamp":1791206943392,"version":"4.1.0"},"reference-count":36,"publisher":"The Company of Biologists","issue":"15","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:p>Oscillations are prevalent in natural systems. A gene expression oscillator, called the segmentation clock, controls segmentation of precursors of the vertebral column. Genes belonging to the Hes\/her family encode the only conserved oscillating genes in all analyzed vertebrate species. Hes\/Her proteins form dimers and negatively autoregulate their own transcription. Here, we developed a stochastic two-dimensional multicellular computational model to elucidate how the dynamics, i.e. period, amplitude and synchronization, of the segmentation clock are regulated. We performed parameter searches to demonstrate that autoregulatory negative-feedback loops of the redundant repressor Her dimers can generate synchronized gene expression oscillations in wild-type embryos and reproduce the dynamics of the segmentation oscillator in different mutant conditions. Our model also predicts that synchronized oscillations can be robustly generated as long as the half-lives of the repressor dimers are shorter than 6 minutes. We validated this prediction by measuring, for the first time, the half-life of Her7 protein as 3.5 minutes. These results demonstrate the importance of building biologically realistic stochastic models to test biological models more stringently and make predictions for future experimental studies.<\/jats:p>","DOI":"10.1242\/dev.093278","type":"journal-article","created":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T07:20:22Z","timestamp":1373959222000},"page":"3244-3253","source":"Crossref","is-referenced-by-count":56,"title":["Short-lived Her proteins drive robust synchronized oscillations in the zebrafish segmentation clock"],"prefix":"10.1242","volume":"140","author":[{"given":"Ahmet","family":"Ay","sequence":"first","affiliation":[{"name":"Department of Mathematics, Colgate University, Hamilton, NY 13346, USA."},{"name":"Department of Biology, Colgate University, Hamilton, NY 13346, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Knierer","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Sperlea","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Colgate University, Hamilton, NY 13346, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Holland","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Colgate University, Hamilton, NY 13346, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ertu\u011frul M.","family":"\u00d6zbudak","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042607232318800_R1","doi-asserted-by":"crossref","first-page":"214107","DOI":"10.1063\/1.2799998","article-title":"A modified next reaction method for simulating chemical systems with time dependent propensities and delays","volume":"127","author":"Anderson","year":"2007","journal-title":"J. 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