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We found previously that UNC-6 (netrin) signals permissively through UNC-40 (DCC) and UNC-5 receptors to prevent anterior displacement of the ray 1 sensillum in the C. elegans male tail. UNC-6\/UNC-40 signalling functions in parallel with SMP-1 (semaporin 1)\/PLX-1 (plexin) signalling to prevent this defect. Here, we report that a deletion allele of pvf-1, which encodes a VEGF-related protein, causes no ray 1 defects, but enhances ray 1 defects of a plx-1 mutant, and unexpectedly also suppresses unc-6(ev400)-null mutant ray 1 defects. These mutant ray 1 inductive and suppressive effects are mimicked by the ability of unc-40(+) and ced-10(gain-of-function) multi-copy transgene arrays to induce ray 1 defects or suppress unc-6 mutant ray 1 defects, depending on their dosage, suggesting the pvf-1 mutation causes UNC-40 overactivity that interferes with signalling but is partially sensitive to UNC-6. Additional data suggest PVF-1 functions through four VEGF receptor-related proteins and inhibits only CED-10 (a GTPase), but not MIG-2-dependent UNC-40 activity, even though UNC-40 functions through both GTPases to position ray 1. pvf-1 and receptor mutant ray 1 defects are rescued by transgenes expressing mouse VEGF164 and human VEGF receptors, respectively. These data report the first case of VEGF-induced inhibition of the netrin signalling and a molecular conservation of VEGF function from worms to humans.<\/jats:p>","DOI":"10.1242\/dev.095190","type":"journal-article","created":{"date-parts":[[2013,9,5]],"date-time":"2013-09-05T04:51:10Z","timestamp":1378356670000},"page":"4020-4030","source":"Crossref","is-referenced-by-count":13,"title":["<i>C. elegans<\/i>PVF-1 inhibits permissive UNC-40 signalling through CED-10 GTPase to position the male ray 1 sensillum"],"prefix":"10.1242","volume":"140","author":[{"given":"Gratien","family":"Dalpe","sequence":"first","affiliation":[{"name":"Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Tarsitano","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Biophysics, CNR, Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Graziella","family":"Persico","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Biophysics, CNR, Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Culotti","sequence":"additional","affiliation":[{"name":"Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada."},{"name":"Department of Molecular Biology, University of Toronto, Toronto, Ontario, M5S 1SA, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042607211953400_R1","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1038\/386838a0","article-title":"The mouse rostral cerebellar malformation gene encodes an UNC-5-like protein","volume":"386","author":"Ackerman","year":"1997","journal-title":"Nature"},{"key":"2021042607211953400_R2","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.1242\/dev.122.8.2517","article-title":"Stage-specific accumulation of the terminal differentiation factor LIN-29 during Caenorhabditis elegans development","volume":"122","author":"Bettinger","year":"1996","journal-title":"Development"},{"key":"2021042607211953400_R3","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1006\/dbio.1999.9491","article-title":"The gon-1 gene is required for gonadal morphogenesis in Caenorhabditis elegans","volume":"216","author":"Blelloch","year":"1999","journal-title":"Dev. 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