{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:55:14Z","timestamp":1790171714483,"version":"4.1.0"},"reference-count":37,"publisher":"The Company of Biologists","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination and epithelial cell movement within the plane of an epithelium are associated with a change in cellular phenotype. However, how this positional change is linked to differentiation remains unknown. Using the developing mouse pancreas as a model system, we show that \u03b2 cell delamination and differentiation are two independent events, which are controlled by Cdc42\/N-WASP signaling. Specifically, we show that expression of constitutively active Cdc42 in \u03b2 cells inhibits \u03b2 cell delamination and differentiation. These processes are normally associated with junctional actin and cell-cell junction disassembly and the expression of fate-determining transcription factors, such as Isl1 and MafA. Mechanistically, we demonstrate that genetic ablation of N-WASP in \u03b2 cells expressing constitutively active Cdc42 partially restores both delamination and \u03b2 cell differentiation. These findings elucidate how junctional actin dynamics via Cdc42\/N-WASP signaling cell-autonomously control not only epithelial delamination but also cell differentiation during mammalian organogenesis.<\/jats:p>","DOI":"10.1242\/dev.100297","type":"journal-article","created":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T12:23:08Z","timestamp":1390306988000},"page":"685-696","source":"Crossref","is-referenced-by-count":50,"title":["Cdc42\/N-WASP signaling links actin dynamics to pancreatic \u03b2 cell delamination and differentiation"],"prefix":"10.1242","volume":"141","author":[{"given":"Gokul","family":"Kesavan","sequence":"first","affiliation":[{"name":"Stem Cell Center, Department of Laboratory Medicine, Lund University, BMC B10 Klinikgatan 26, SE-22184 Lund, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Lieven","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Department of Laboratory Medicine, Lund University, BMC B10 Klinikgatan 26, SE-22184 Lund, Sweden."},{"name":"The Danish Stem Cell Center, University of Copenhagen, Blegdamsvej 3B, Building 6, 4th floor, DK-2200 Copenhagen N, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anant","family":"Mamidi","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Department of Laboratory Medicine, Lund University, BMC B10 Klinikgatan 26, SE-22184 Lund, Sweden."},{"name":"The Danish Stem Cell Center, University of Copenhagen, Blegdamsvej 3B, Building 6, 4th floor, DK-2200 Copenhagen N, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zarah L\u00f6f","family":"\u00d6hlin","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Department of Laboratory Medicine, Lund University, BMC B10 Klinikgatan 26, SE-22184 Lund, Sweden."},{"name":"The Danish Stem Cell Center, University of Copenhagen, Blegdamsvej 3B, Building 6, 4th floor, DK-2200 Copenhagen N, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny Kristina","family":"Johansson","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Department of Laboratory Medicine, Lund University, BMC B10 Klinikgatan 26, SE-22184 Lund, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Chun","family":"Li","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Department of Laboratory Medicine, Lund University, BMC B10 Klinikgatan 26, SE-22184 Lund, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Lommel","sequence":"additional","affiliation":[{"name":"Institute for Cell Biology, University of Bonn, Ulrich-Haberland-Strasse 61a, 53121 Bonn, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas Uwe","family":"Greiner","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Department of Laboratory Medicine, Lund University, BMC B10 Klinikgatan 26, SE-22184 Lund, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Semb","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Department of Laboratory Medicine, Lund University, BMC B10 Klinikgatan 26, SE-22184 Lund, Sweden."},{"name":"The Danish Stem Cell Center, University of Copenhagen, Blegdamsvej 3B, Building 6, 4th floor, DK-2200 Copenhagen N, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042607574934700_R1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1677\/JOE-08-0063","article-title":"MafA is a dedicated activator of the insulin gene in vivo.","volume":"198","author":"Artner","year":"2008","journal-title":"J. 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