{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:53:27Z","timestamp":1790823207965,"version":"4.1.0"},"reference-count":20,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1989,7,1]],"date-time":"1989-07-01T00:00:00Z","timestamp":615254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1989,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>In order to study migration of neurons in vitro, we cultured microexplants of the newborn mouse cerebellum outer layer, which is rich in immature granule cells, on a substratum double-coated with poly-L-lysine and laminin. The granule cells first migrated away from the explant along radially oriented parallel bundles of their neurites, thus displaying typical contact guidance. Then, in almost all explants, they changed their orientation by 90\u00b0 to extend cell processes and translocate perpendicular to the radial neurites. Orientation and migration of neurons perpendicular to the aligned parallel structure is a novel type of contact-guided cell behavior, and may have interesting implications in migration of neurons in the cerebellum and other parts of the nervous system.<\/jats:p>","DOI":"10.1242\/dev.106.3.441","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:40:05Z","timestamp":1619397605000},"page":"441-447","source":"Crossref","is-referenced-by-count":35,"title":["Paradoxical perpendicular contact guidance displayed by mouse cerebellar granule cell neurons <i>in vitro<\/i>"],"prefix":"10.1242","volume":"106","author":[{"given":"Norio","family":"Nakatsuji","sequence":"first","affiliation":[{"name":"1 Division of Developmental Biology, Meiji Institute of Health Science, 540 Naruda, Odawara 250, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isao","family":"Nagata","sequence":"additional","affiliation":[{"name":"2 Department of Genetics, Tokyo Metropolitan Institute for Neurosciences, 2\u20136 Musashidai, Fuchu, Tokyo 183, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1989,7,1]]},"reference":[{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1126\/science.3413496","article-title":"Application of the quail-chick chimera system to the study of brain development and behavior","volume":"241","author":"Balaban","year":"1988","journal-title":"Science"},{"key":"2023040617545523700_","first-page":"247","article-title":"Contact guidance of cultured tissue cells: a survey of potentially relevant properties of the substratum","volume-title":"In Cell Behaviour","author":"Dunn","year":"1982"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"1928","DOI":"10.1523\/JNEUROSCI.07-06-01928.1987","article-title":"Glial-guided granule neuron migration in vitro: A high-resolution time-lapse video microscopic study","volume":"7","author":"Edmondson","year":"1987","journal-title":"J. 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Sci. U.S.A"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"1728","DOI":"10.1523\/JNEUROSCI.08-05-01728.1988","article-title":"Cytology and neuron-glial apposition of migrating cerebellar granule cells in vitro","volume":"8","author":"Gregory","year":"1988","journal-title":"J. Neurosci"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1523\/JNEUROSCI.06-03-00867.1986","article-title":"Migration of neuroblasts along preexisting axonal tracts during prenatal cerebellar development","volume":"6","author":"Hynes","year":"1986","journal-title":"J. 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Zool"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1038\/307453a0","article-title":"Experimental manipulation of a contact guidance system in amphibian gastrulation by mechanical tension","volume":"307","author":"Nakatsuji","year":"1984","journal-title":"Nature, Lond"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1002\/cne.901410303","article-title":"Neuron-glia relationship during granule cell migration in developing cerebellar cortex. A Golgi and electronmicroscopic study in Macacus rhesus","volume":"141","author":"Rakic","year":"1971","journal-title":"J. comp. 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Neuroimmunol"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"1067","DOI":"10.1126\/science.6844928","article-title":"Postnatally induced formation of the corpus callosum in acallosal mice on glia-coated cellulose bridges","volume":"220","author":"Silver","year":"1983","journal-title":"Science"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1002\/cne.901890106","article-title":"A mechanism for the guidance and topographic patterning of retinal ganglion cell axons","volume":"189","author":"Silver","year":"1980","journal-title":"J. comp. Neurol"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/cne.901850102","article-title":"Axonal guidance during embryogenesis and regeneration in the spinal cord of the newt: The blueprint hypothesis of neuronal pathway patterning","volume":"185","author":"Singer","year":"1979","journal-title":"J. comp. Neurol"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"1342","DOI":"10.1126\/science.3137660","article-title":"Clonally related cortical cells show several migration patterns","volume":"241","author":"Walsh","year":"1988","journal-title":"Science"},{"key":"2023040617545523700_","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1242\/jcs.66.1.205","article-title":"An analysis of in vivo cell migration during teleost fin morphogenesis","volume":"66","author":"Wood","year":"1984","journal-title":"J. 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