{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T04:28:06Z","timestamp":1780633686261,"version":"3.54.1"},"reference-count":10,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1989,11,1]],"date-time":"1989-11-01T00:00:00Z","timestamp":625881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1989,11,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>We are studying the development of handedness, in particular the relationships between handed structures with bilateral symmetry, for example the limbs, and those with lateral asymmetry, such as the heart, lungs and gut. Asymmetric (unilateral) developmental limb abnormalities can be induced by chemical treatment of mouse embryos, either in utero by acetazolamide, or in culture by misonidazole. We have examined these effects in mice homozygous for the iv gene. The development of bilateral symmetry in iv\/iv mice is normal, but the control of asymmetry appears to be random, that is 50 % develop normally (situs solitus), 50 % with laterally inverted viscera (situs inversus). We find that the handedness of induced asymmetric limb defects is highly correlated with embryonic visceral situs. Right limb defects are induced in situs solitus embryos, left-sided defects in situs inversus. This suggests that the mechanism of induction of asymmetric defects is not related to any intrinsic difference between the development of left and right limbs, but is connected to visceral asymmetry. In addition, the high correlation of limb defects with situs was observed in culture as well as in utero suggesting that the maternal environment plays no role in the development of asymmetry.<\/jats:p>","DOI":"10.1242\/dev.107.3.637","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:44:13Z","timestamp":1619397853000},"page":"637-642","source":"Crossref","is-referenced-by-count":34,"title":["The development of asymmetry: the sidedness of drug-induced limb abnormalities is reversed in situs inversus mice"],"prefix":"10.1242","volume":"107","author":[{"given":"Nigel A.","family":"Brown","sequence":"first","affiliation":[{"name":"l MRC Experimental Embryology and Teratology Unit, St George\u2019s Hospital Medical School, Cranmer Terrace, London SW17 ORE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine I.","family":"Hoyle","sequence":"additional","affiliation":[{"name":"l MRC Experimental Embryology and Teratology Unit, St George\u2019s Hospital Medical School, Cranmer Terrace, London SW17 ORE, UK"},{"name":"University College and Middlesex Hospital Medical School 2 Department of Anatomy and Developmental Biology , , Cleveland Street, London W1P6DB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afshan","family":"McCarthy","sequence":"additional","affiliation":[{"name":"l MRC Experimental Embryology and Teratology Unit, St George\u2019s Hospital Medical School, Cranmer Terrace, London SW17 ORE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis","family":"Wolpert","sequence":"additional","affiliation":[{"name":"University College and Middlesex Hospital Medical School 2 Department of Anatomy and Developmental Biology , , Cleveland Street, London W1P6DB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1989,11,1]]},"reference":[{"key":"2023030315563822400_","article-title":"The development of handedness: left\/right asymmetry","volume-title":"Development (submitted)","author":"Brown","year":"1989"},{"key":"2023030315563822400_","first-page":"404","article-title":"Tissue oxygen as a determinant of axially asymmetric teratologic responses: Misonidazole as a marker for hypoxic cells","volume":"5","author":"Coakley","year":"1986","journal-title":"Human Toxicol"},{"key":"2023030315563822400_","first-page":"83","article-title":"Post-implantation embryo culture for studies of teratogenesis","volume-title":"In Biochemical Toxicology, A Practical Approach","author":"Freeman","year":"1987"},{"key":"2023030315563822400_","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/0041-008X(86)90206-1","article-title":"On the capacity of nitroheterocyclic compounds to elicit an unusual axial asymmetry in cultured rat embryos","volume":"82","author":"Greenaway","year":"1986","journal-title":"Toxicol. &lt;fc Appl. Pharmacol"},{"key":"2023030315563822400_","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1093\/oxfordjournals.jhered.a106870","article-title":"Visceral inversion and associated anomalies in the mouse","volume":"50","author":"Hummel","year":"1959","journal-title":"J. Hered"},{"key":"2023030315563822400_","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1093\/oxfordjournals.jhered.a108749","article-title":"Random determination of a developmental process: reversal of normal visceral asymmetry in the mouse","volume":"67","author":"Layton","year":"1976","journal-title":"J. Hered."},{"key":"2023030315563822400_","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1126\/science.149.3681.306.b","article-title":"Deformity of forelimb in rats: association with high doses of acetazolamide","volume":"149","author":"Layton","year":"1965","journal-title":"Science"},{"key":"2023030315563822400_","doi-asserted-by":"publisher","first-page":"A37","DOI":"10.1002\/tera.1420090304","article-title":"Detection of visceral alterations in mammalian fetuses","volume":"9","author":"Staples","year":"1974","journal-title":"Teratology"},{"key":"2023030315563822400_","first-page":"62","article-title":"Refinements in rapid clearing technique in the KOH-Alizarin Red S method for fetal bone","volume":"39","author":"Staples","year":"1964","journal-title":"Stain Technol"},{"key":"2023030315563822400_","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1002\/tera.1420010107","article-title":"Teratogenic action of carbonic anhydrase inhibitors in the rat","volume":"1","author":"Wilson","year":"1968","journal-title":"Teratology"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/107\/3\/637\/2598477\/develop_107_3_637.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/107\/3\/637\/2598477\/develop_107_3_637.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T15:56:42Z","timestamp":1677859002000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/107\/3\/637\/36539\/The-development-of-asymmetry-the-sidedness-of-drug"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,11,1]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,11,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.107.3.637","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,11,1]]}}}