{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T16:34:21Z","timestamp":1776875661899,"version":"3.51.2"},"reference-count":68,"publisher":"The Company of Biologists","issue":"15","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:p>In this study we examine the consequences of altering Hand1 phosphoregulation in the developing neural crest cells (NCCs) of mice. Whereas Hand1 deletion in NCCs reveals a nonessential role for Hand1 in craniofacial development and embryonic survival, altering Hand1 phosphoregulation, and consequently Hand1 dimerization affinities, in NCCs results in severe mid-facial clefting and neonatal death. Hand1 phosphorylation mutants exhibit a non-cell-autonomous increase in pharyngeal arch cell death accompanied by alterations in Fgf8 and Shh pathway expression. Together, our data indicate that the extreme distal pharyngeal arch expression domain of Hand1 defines a novel bHLH-dependent activity, and that disruption of established Hand1 dimer phosphoregulation within this domain disrupts normal craniofacial patterning.<\/jats:p>","DOI":"10.1242\/dev.107680","type":"journal-article","created":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T11:19:14Z","timestamp":1406027954000},"page":"3050-3061","source":"Crossref","is-referenced-by-count":31,"title":["Hand1 phosphoregulation within the distal arch neural crest is essential for craniofacial morphogenesis"],"prefix":"10.1242","volume":"141","author":[{"given":"Beth A.","family":"Firulli","sequence":"first","affiliation":[{"name":"Riley Heart Research Center, Herman B Wells Center for Pediatric Research, Division of Pediatric Cardiology, Departments of Anatomy and Medical, Biochemistry, and Molecular Genetics, Indiana Medical School, 1044 W. Walnut Street, Indianapolis, IN 46202-5225, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robyn K.","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy and the Center for Translational Musculoskeletal Research, School of Health and Rehabilitation Science, Indiana University, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua W.","family":"Vincentz","sequence":"additional","affiliation":[{"name":"Riley Heart Research Center, Herman B Wells Center for Pediatric Research, Division of Pediatric Cardiology, Departments of Anatomy and Medical, Biochemistry, and Molecular Genetics, Indiana Medical School, 1044 W. Walnut Street, Indianapolis, IN 46202-5225, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E.","family":"Clouthier","sequence":"additional","affiliation":[{"name":"Department of Craniofacial Biology, University of Colorado Anschutz Medical Campus, 12801 E 17th Avenue, Rm. 11-109, MS 8120, Aurora, CO 80045, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony B.","family":"Firulli","sequence":"additional","affiliation":[{"name":"Riley Heart Research Center, Herman B Wells Center for Pediatric Research, Division of Pediatric Cardiology, Departments of Anatomy and Medical, Biochemistry, and Molecular Genetics, Indiana Medical School, 1044 W. Walnut Street, Indianapolis, IN 46202-5225, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021052800020258700_DEV107680C1","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1111\/j.1440-169X.2008.01069.x","article-title":"Retinoic acid affects craniofacial patterning by changing Fgf8 expression in the pharyngeal ectoderm","volume":"50","author":"Abe","year":"2008","journal-title":"Dev. Growth Differ."},{"key":"2021052800020258700_DEV107680C2","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.ydbio.2004.05.028","article-title":"Shh and Fgf8 act synergistically to drive cartilage outgrowth during cranial development","volume":"273","author":"Abzhanov","year":"2004","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C3","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1111\/j.1741-4520.2007.00162.x","article-title":"Cross-regulatory interactions between Fgf8 and Shh in the avian frontonasal prominence","volume":"47","author":"Abzhanov","year":"2007","journal-title":"Congenit. Anom. Kyoto"},{"key":"2021052800020258700_DEV107680C4","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.ydbio.2007.07.036","article-title":"Hand transcription factors cooperatively regulate development of the distal midline mesenchyme","volume":"310","author":"Barbosa","year":"2007","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C5","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1387\/ijdb.082747rb","article-title":"A Twist of insight - the role of Twist-Family bHLH factors in development","volume":"53","author":"Barnes","year":"2009","journal-title":"Int. J. Dev. Biol."},{"key":"2021052800020258700_DEV107680C6","doi-asserted-by":"publisher","first-page":"3086","DOI":"10.1002\/dvdy.22428","article-title":"Analysis of the Hand1 cell lineage reveals novel contributions to cardiovascular, neural crest, extra-embryonic, and lateral mesoderm derivatives","volume":"239","author":"Barnes","year":"2010","journal-title":"Dev. Dyn."},{"key":"2021052800020258700_DEV107680C7","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1161\/CIRCRESAHA.110.233171","article-title":"Hand2 loss-of-function in Hand1-expressing cells reveals distinct roles in epicardial and coronary vessel development","volume":"108","author":"Barnes","year":"2011","journal-title":"Circ. Res."},{"key":"2021052800020258700_DEV107680C8","doi-asserted-by":"publisher","first-page":"2249","DOI":"10.1242\/dev.056929","article-title":"Downregulation of Dlx5 and Dlx6 expression by Hand2 is essential for initiation of tongue morphogenesis","volume":"138","author":"Barron","year":"2011","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C9","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/j.ydbio.2009.04.034","article-title":"Requirement for Twist1 in frontonasal and skull vault development in the mouse embryo","volume":"331","author":"Bildsoe","year":"2009","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0070-2153(05)73001-5","article-title":"The molecular origins of species-specific facial pattern","volume":"73","author":"Brugmann","year":"2006","journal-title":"Curr. Top. Dev. Biol."},{"key":"2021052800020258700_DEV107680C11","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1002\/bies.20313","article-title":"A twisted hand: bHLH protein phosphorylation and dimerization regulate limb development","volume":"27","author":"Cai","year":"2005","journal-title":"BioEssays"},{"key":"2021052800020258700_DEV107680C12","doi-asserted-by":"crossref","first-page":"3145","DOI":"10.1242\/dev.128.16.3145","article-title":"Dimerization partners determine the activity of the Twist bHLH protein during Drosophila mesoderm development","volume":"128","author":"Castanon","year":"2001","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C13","doi-asserted-by":"publisher","first-page":"2353","DOI":"10.1002\/dvdy.20833","article-title":"Recent advances in craniofacial morphogenesis","volume":"235","author":"Chai","year":"2006","journal-title":"Dev. Dyn."},{"key":"2021052800020258700_DEV107680C14","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1101\/gad.9.6.686","article-title":"Twist is required in head mesenchyme for cranial neural tube morphogenesis","volume":"9","author":"Chen","year":"1995","journal-title":"Genes Dev."},{"key":"2021052800020258700_DEV107680C15","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.ydbio.2011.10.019","article-title":"Influence of mesodermal Fgf8 on the differentiation of neural crest-derived postganglionic neurons","volume":"361","author":"Chen","year":"2012","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C16","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1006\/dbio.1999.9527","article-title":"Signaling pathways crucial for craniofacial development revealed by endothelin-A receptor-deficient mice","volume":"217","author":"Clouthier","year":"2000","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C17","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1016\/S0012-1606(03)00128-3","article-title":"Cell-autonomous and nonautonomous actions of endothelin-A receptor signaling in craniofacial and cardiovascular development","volume":"261","author":"Clouthier","year":"2003","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C18","doi-asserted-by":"publisher","first-page":"2962","DOI":"10.1002\/ajmg.a.33568","article-title":"Regulation of facial morphogenesis by endothelin signaling: insights from mice and fish","volume":"152A","author":"Clouthier","year":"2010","journal-title":"Am. J. Med. Genet. A"},{"key":"2021052800020258700_DEV107680C19","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1002\/ajmg.c.31376","article-title":"Understanding the basis of auriculocondylar syndrome: insights from human, mouse and zebrafish genetic studies","volume":"163","author":"Clouthier","year":"2013","journal-title":"Am. J. Med. Genet. C Semin. Med. Genet."},{"key":"2021052800020258700_DEV107680C20","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.ydbio.2009.04.021","article-title":"Sonic hedgehog signalling inhibits palatogenesis and arrests tooth development in a mouse model of the nevoid basal cell carcinoma syndrome","volume":"331","author":"Cobourne","year":"2009","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C21","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1016\/j.ydbio.2013.02.022","article-title":"Pattern and polarity in the development and evolution of the gnathostome jaw: both conservation and heterotopy in the branchial arches of the shark, Scyliorhinus canicula","volume":"377","author":"Compagnucci","year":"2013","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C22","doi-asserted-by":"publisher","first-page":"1334","DOI":"10.1002\/dvdy.20717","article-title":"Twist1 dimer selection regulates cranial suture patterning and fusion","volume":"235","author":"Connerney","year":"2006","journal-title":"Dev. Dyn."},{"key":"2021052800020258700_DEV107680C23","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.ydbio.2008.03.037","article-title":"Twist1 homodimers enhance FGF responsiveness of the cranial sutures and promote suture closure","volume":"318","author":"Connerney","year":"2008","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C25","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1016\/S0960-9822(07)00562-3","article-title":"Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase","volume":"8","author":"Danielian","year":"1998","journal-title":"Curr. Biol."},{"key":"2021052800020258700_DEV107680C26","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1002\/jez.b.21205","article-title":"Tweaking the hinge and caps: testing a model of the organization of jaws","volume":"310B","author":"Depew","year":"2008","journal-title":"J. Exp. Zoolog. B Mol. Dev. Evol."},{"key":"2021052800020258700_DEV107680C27","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.1002\/dvdy.20796","article-title":"21st century neontology and the comparative development of the vertebrate skull","volume":"235","author":"Depew","year":"2006","journal-title":"Dev. Dyn."},{"key":"2021052800020258700_DEV107680C28","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1006\/dbio.2002.0696","article-title":"FGF signaling regulates expression of Tbx2, Erm, Pea3, and Pax3 in the early nasal region","volume":"247","author":"Firnberg","year":"2002","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C29","doi-asserted-by":"publisher","first-page":"2641","DOI":"10.2174\/092986708785908987","article-title":"Phosphoregulation of Twist1 provides a mechanism of cell fate control","volume":"15","author":"Firulli","year":"2008","journal-title":"Curr. Med. Chem."},{"key":"2021052800020258700_DEV107680C30","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1038\/ng0398-266","article-title":"Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1","volume":"18","author":"Firulli","year":"1998","journal-title":"Nat. Genet."},{"key":"2021052800020258700_DEV107680C31","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1016\/S1097-2765(03)00425-8","article-title":"PKA, PKC and the protein phosphatase 2A influence HAND factor function: a mechanisms for tissue specific transcriptional regulation","volume":"12","author":"Firulli","year":"2003","journal-title":"Mol. Cell"},{"key":"2021052800020258700_DEV107680C32","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1038\/ng1525","article-title":"Altered Twist1 and Hand2 dimerization is associated with Saethre-Chotzen syndrome and limb abnormalities","volume":"37","author":"Firulli","year":"2005","journal-title":"Nat. Genet."},{"key":"2021052800020258700_DEV107680C33","doi-asserted-by":"publisher","first-page":"27536","DOI":"10.1074\/jbc.M702613200","article-title":"Mutations within helix I of Twist1 result in distinct limb defects and variation of DNA binding affinities","volume":"282","author":"Firulli","year":"2007","journal-title":"J. Biol. Chem."},{"key":"2021052800020258700_DEV107680C34","doi-asserted-by":"publisher","first-page":"2748","DOI":"10.1002\/dvdy.22402","article-title":"Analysis of a Hand1 hypomorphic allele reveals a critical threshold for embryonic viability","volume":"239","author":"Firulli","year":"2010","journal-title":"Dev. Dyn."},{"key":"2021052800020258700_DEV107680C35","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1111\/j.1525-142X.2011.00511.x","article-title":"Satb2, modularity, and the evolvability of the vertebrate jaw","volume":"13","author":"Fish","year":"2011","journal-title":"Evol. Dev."},{"key":"2021052800020258700_DEV107680C69","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/j.semcdb.2010.01.008","article-title":"Evolving maps in craniofacial development. Semin","volume":"21","author":"Gitton","year":"2010","journal-title":"Cell Dev. Biol."},{"key":"2021052800020258700_DEV107680C36","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.ydbio.2012.11.014","article-title":"Fgf8 dosage determines midfacial integration and polarity within the nasal and optic capsules","volume":"374","author":"Griffin","year":"2013","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C37","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.ydbio.2008.03.036","article-title":"Conditional deletion of Hand2 reveals critical functions in neurogenesis and cell type-specific gene expression for development of neural crest-derived noradrenergic sympathetic ganglion neurons","volume":"319","author":"Hendershot","year":"2008","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C38","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1007\/s00429-004-0412-3","article-title":"Twist is an integrator of SHH, FGF, and BMP signaling","volume":"209","author":"Hornik","year":"2004","journal-title":"Anat. Embryol."},{"key":"2021052800020258700_DEV107680C39","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/j.ydbio.2004.09.034","article-title":"Mechanisms and perspectives on differentiation of autonomic neurons","volume":"277","author":"Howard","year":"2005","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C40","doi-asserted-by":"publisher","first-page":"1749","DOI":"10.1242\/dev.00397","article-title":"A zone of frontonasal ectoderm regulates patterning and growth in the face","volume":"130","author":"Hu","year":"2003","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C41","doi-asserted-by":"publisher","first-page":"1454","DOI":"10.1093\/hmg\/ddm096","article-title":"Ectopic sonic hedgehog signaling impairs telencephalic dorsal midline development: implication for human holoprosencephaly","volume":"16","author":"Huang","year":"2007","journal-title":"Hum. Mol. Genet."},{"key":"2021052800020258700_DEV107680C42","first-page":"401","article-title":"TWIST and Saethre-Chotzen syndrome","volume-title":"Inborn Errors of Development","author":"Jabs","year":"2004"},{"key":"2021052800020258700_DEV107680C43","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1101\/gad.1190304","article-title":"Hedgehog signaling in the neural crest cells regulates the patterning and growth of facial primordia","volume":"18","author":"Jeong","year":"2004","journal-title":"Genes Dev."},{"key":"2021052800020258700_DEV107680C44","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.1002\/dvdy.20646","article-title":"Development of the upper lip: morphogenetic and molecular mechanisms","volume":"235","author":"Jiang","year":"2006","journal-title":"Dev. Dyn."},{"key":"2021052800020258700_DEV107680C45","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1016\/j.ydbio.2013.07.007","article-title":"SP8 regulates signaling centers during craniofacial development","volume":"381","author":"Kasberg","year":"2013","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C46","doi-asserted-by":"publisher","first-page":"S13","DOI":"10.1097\/00005344-199506263-00005","article-title":"Impaired development of the thyroid and thymus in endothelin-1 knockout mice","volume":"26","author":"Kurihara","year":"1995","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"2021052800020258700_DEV107680C47","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1242\/dev.028167","article-title":"Sonic hedgehog signaling regulates reciprocal epithelial-mesenchymal interactions controlling palatal outgrowth","volume":"136","author":"Lan","year":"2009","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C48","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1093\/toxsci\/kfn076","article-title":"Dose- and route-dependent teratogenicity, toxicity, and pharmacokinetic profiles of the hedgehog signaling antagonist cyclopamine in the mouse","volume":"104","author":"Lipinski","year":"2008","journal-title":"Toxicol. Sci."},{"key":"2021052800020258700_DEV107680C49","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1002\/bdra.20656","article-title":"Cleft lip and palate results from Hedgehog signaling antagonism in the mouse: phenotypic characterization and clinical implications","volume":"88","author":"Lipinski","year":"2010","journal-title":"Birth Defects Res. A Clin. Mol. Teratol."},{"key":"2021052800020258700_DEV107680C50","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1016\/j.ydbio.2006.10.014","article-title":"A spatial and temporal map of FGF\/Erk1\/2 activity and response repertoires in the early chick embryo","volume":"302","author":"Lunn","year":"2007","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C51","doi-asserted-by":"publisher","first-page":"6361","DOI":"10.1242\/dev.00850","article-title":"Ablation of specific expression domains reveals discrete functions of ectoderm- and endoderm-derived FGF8 during cardiovascular and pharyngeal development","volume":"130","author":"Macatee","year":"2003","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C52","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1128\/MCB.20.2.429-440.2000","article-title":"Helix-loop-helix proteins: regulators of transcription in Eucaryotic organisms","volume":"20","author":"Massari","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"2021052800020258700_DEV107680C53","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1242\/dev.01562","article-title":"The Hand1 and Hand2 transcription factors regulate expansion of the embryonic cardiac ventricles in a gene dosage-dependent manner","volume":"132","author":"McFadden","year":"2005","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C54","doi-asserted-by":"publisher","first-page":"2605","DOI":"10.1242\/dev.040048","article-title":"Molecular mechanisms of cranial neural crest cell migration and patterning in craniofacial development","volume":"137","author":"Minoux","year":"2010","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C55","doi-asserted-by":"crossref","first-page":"4465","DOI":"10.1242\/dev.126.20.4465","article-title":"Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed","volume":"126","author":"Minowada","year":"1999","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C56","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1038\/82601","article-title":"Fgf8 is required for outgrowth and patterning of the limbs","volume":"26","author":"Moon","year":"2000","journal-title":"Nat. Genet."},{"key":"2021052800020258700_DEV107680C57","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1111\/j.1469-7580.2005.00473.x","article-title":"Relations and interactions between cranial mesoderm and neural crest populations","volume":"207","author":"Noden","year":"2005","journal-title":"J. Anat."},{"key":"2021052800020258700_DEV107680C58","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/S0925-4773(01)00456-7","article-title":"Tight transcriptional control of the ETS domain factors Erm and Pea3 by Fgf signaling during early zebrafish development","volume":"107","author":"Raible","year":"2001","journal-title":"Mech. Dev."},{"key":"2021052800020258700_DEV107680C59","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1101\/gad.5.8.1333","article-title":"Expression of a retinoic acid response element-hsplacZ transgene defines specific domains of transcriptional activity during mouse embryogenesis","volume":"5","author":"Rossant","year":"1991","journal-title":"Genes Dev."},{"key":"2021052800020258700_DEV107680C60","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.ydbio.2009.01.005","article-title":"Elucidating timing and function of endothelin-A receptor signaling during craniofacial development using neural crest cell-specific gene deletion and receptor antagonism","volume":"328","author":"Ruest","year":"2009","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C61","doi-asserted-by":"publisher","first-page":"4413","DOI":"10.1242\/dev.01291","article-title":"Endothelin-A receptor-dependent and -independent signaling pathways in establishing mandibular identity","volume":"131","author":"Ruest","year":"2004","journal-title":"Development"},{"key":"2021052800020258700_DEV107680C62","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1101\/gad.1533407","article-title":"RDH10 is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development","volume":"21","author":"Sandell","year":"2007","journal-title":"Genes Dev."},{"key":"2021052800020258700_DEV107680C63","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1006\/dbio.2002.0699","article-title":"Twist function is required for the morphogenesis of the cephalic neural tube and the differentiation of the cranial neural crest cells in the mouse embryo","volume":"247","author":"Soo","year":"2002","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C64","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1597\/08-088.1","article-title":"The frequency of palatal anomalies in Saethre-Chotzen syndrome","volume":"46","author":"Stoler","year":"2009","journal-title":"Cleft Palate Craniofac. J."},{"key":"2021052800020258700_DEV107680C65","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1159\/000088130","article-title":"Specification and patterning of neural crest cells during craniofacial development","volume":"66","author":"Trainor","year":"2005","journal-title":"Brain Behav. Evol."},{"key":"2021052800020258700_DEV107680C66","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.ydbio.2008.04.037","article-title":"An absence of Twist1 results in aberrant cardiac neural crest morphogenesis","volume":"320","author":"Vincentz","year":"2008","journal-title":"Dev. Biol."},{"key":"2021052800020258700_DEV107680C67","doi-asserted-by":"publisher","first-page":"2110","DOI":"10.1523\/JNEUROSCI.3584-11.2012","article-title":"A Phox2- and Hand2-dependent Hand1 cis-regulatory element reveals a unique gene dosage requirement for Hand2 during sympathetic neurogenesis","volume":"32","author":"Vincentz","year":"2012","journal-title":"J. Neurosci."},{"key":"2021052800020258700_DEV107680C68","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1006\/dbio.1999.9323","article-title":"A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice","volume":"212","author":"Yamauchi","year":"1999","journal-title":"Dev. Biol."}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/141\/15\/3050\/2073918\/dev107680.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/141\/15\/3050\/2073918\/dev107680.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:49:35Z","timestamp":1717116575000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/141\/15\/3050\/46274\/Hand1-phosphoregulation-within-the-distal-arch"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,1]]},"references-count":68,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2014,8,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.107680","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2014,8,1]]}}}