{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:19:01Z","timestamp":1770981541130,"version":"3.50.1"},"reference-count":12,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1990,2,1]],"date-time":"1990-02-01T00:00:00Z","timestamp":633830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We introduced a mouse tyrosinase minigene, mg-Tyrs-J, in which the authentic genomic 5\u2032 non-coding flanking sequence was fused to a mouse tyrosinase cDNA, into fertilized eggs of albino mice.<\/jats:p>\n               <jats:p>Of the 25 animals that developed from the injected eggs, four mice exhibited pigmented hair and eyes. Histological analysis of the transgenic mice revealed that the melanogenesis was restricted to hair bulbs and eyes.<\/jats:p>\n               <jats:p>These results suggest that this minigene encodes active tyrosinase protein and that its 5\u2032 flanking region contains the sequences regulating expression of mouse tyrosinase gene. This is the first report of a successful expression of tyrosinase gene and of pigment production in transgenic mice.<\/jats:p>","DOI":"10.1242\/dev.108.2.223","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:40:16Z","timestamp":1619397616000},"page":"223-227","source":"Crossref","is-referenced-by-count":83,"title":["Melanization in albino mice transformed by introducing cloned mouse <i>tyrosinase<\/i> gene"],"prefix":"10.1242","volume":"108","author":[{"given":"Satoshi","family":"Tanaka","sequence":"first","affiliation":[{"name":"Biological Institute, Faculty of Science, Tohoku University, Aoba-yama, Sendai 980, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Biological Institute, Faculty of Science, Tohoku University, Aoba-yama, Sendai 980, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakae","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Biological Institute, Faculty of Science, Tohoku University, Aoba-yama, Sendai 980, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuji","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Biological Institute, Faculty of Science, Tohoku University, Aoba-yama, Sendai 980, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1990,2,1]]},"reference":[{"key":"2023050311341394700_DEVELOP_108_2_223C1","doi-asserted-by":"publisher","first-page":"7241","DOI":"10.1073\/pnas.85.19.7241","article-title":"Tyrosinases of mucine melanocytes with mutations at the albino locus","volume":"85","author":"Halaban","year":"1988","journal-title":"Proc. natn. Acad. Sci. U.S.A."},{"key":"2023050311341394700_DEVELOP_108_2_223C2","volume-title":"Manipulating the Mouse Embryo-A Laboratory Manual.","author":"Hogan","year":"1986"},{"key":"2023050311341394700_DEVELOP_108_2_223C3","doi-asserted-by":"publisher","first-page":"1301","DOI":"10.1016\/S0006-291X(88)81370-6","article-title":"Sequence analysis of mouse tyrosinase cDNA and the effect of melanotropin on its gene expression","volume":"153","author":"Kwon","year":"1988","journal-title":"Biochem, Biophys. Res. Comm."},{"key":"2023050311341394700_DEVELOP_108_2_223C4","volume-title":"Molecular Cloning - A Laboratory Manual.","author":"Maniatis","year":"1982"},{"key":"2023050311341394700_DEVELOP_108_2_223C5","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1177\/22.12.1077","article-title":"Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy","volume":"22","author":"Mclean","year":"1974","journal-title":"J. Histochem. 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The c and p loci, and ddpp interaction","volume":"17","author":"Rittenhouse","year":"1968","journal-title":"Devl Biol."},{"key":"2023050311341394700_DEVELOP_108_2_223C9","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4612-6164-3","volume-title":"The Coat Colors of Mice-A Mode! for Mammalian Gene Action and Interaction.","author":"Silvers","year":"1979"},{"key":"2023050311341394700_DEVELOP_108_2_223C10","first-page":"56","volume":"1","author":"Takeuchi","year":"1984","journal-title":"In Structure and Function of Melanin, ed. Jimbow, K. (Fuji-Shoin, Sapporo, Japan)"},{"key":"2023050311341394700_DEVELOP_108_2_223C11","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1266\/jjg.62.271","article-title":"Cloning and sequencing of mouse tyrosinase cDNA","volume":"62","author":"Yamamoto","year":"1987","journal-title":"Jpn. J. Genet."},{"key":"2023050311341394700_DEVELOP_108_2_223C12","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1266\/jjg.64.121","article-title":"Melanin production in cultured albino melanocytes transfected with mouse tyrosinase cDNA","volume":"64","author":"Yamamoto","year":"1989","journal-title":"Jpn. J. Genet."}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/108\/2\/223\/2803388\/develop_108_2_223.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/108\/2\/223\/2803388\/develop_108_2_223.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T11:41:16Z","timestamp":1683114076000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/108\/2\/223\/36372\/Melanization-in-albino-mice-transformed-by"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,2,1]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,2,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.108.2.223","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,2,1]]}}}