{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:35:34Z","timestamp":1758926134933},"reference-count":27,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1990,3,1]],"date-time":"1990-03-01T00:00:00Z","timestamp":636249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Human first trimester extraembryonic tissues of normal and androgenetic origin (molar pregnancies) were investigated for the expression of 6 homeobox genes from the chromosome 12-encoded Hox-3 cluster by non-autoradiographic in situ hybridization with biotinylated RNA probes. By comparative in situ hybridization involving the use of exon- or region-specific RNA probes, analysis included the cellular distribution of alternative Hox-3 transcripts in chorionic villous tissues. A bias in extraembryonic distribution was seen between transcripts of the three most upstream Hox-3 genes (Hox-3.7, -3.6, and -3.1) versus transcripts of the 3 most downstream genes (Hox-3.3, 3.4, and 3.5). Only genes from the latter group are transcribed in human extra-embryonic tissues including extraembryonic tissues of androgenetic origin. Moreover, comparative in situ hybridization showed that distinct alternative transcripts of Hox-3.3, Hox 3.4 and Hox-3.5 are exclusively found in trophoblast cells while others are present in chorionic villous stromal cells as well. These data demonstrate the existence of tissue- and cell-specific use of transcriptional (alternative gene promoters) or post-transcriptional (alternative splicing) regulation of homeobox genes in extraembryonic tissues.<\/jats:p>","DOI":"10.1242\/dev.108.3.471","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:40:16Z","timestamp":1619397616000},"page":"471-477","source":"Crossref","is-referenced-by-count":25,"title":["The three most downstream genes of the Hox-3 cluster are expressed in human extraembryonic tissues including trophoblast of androgenetic origin"],"prefix":"10.1242","volume":"108","author":[{"given":"Cees B. M.","family":"Oudejans","sequence":"first","affiliation":[{"name":"Free University 1 The Department of Molecular Pathology , , De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Pannese","sequence":"additional","affiliation":[{"name":"2 International Institute of Genetics and Biophysics, CNR, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Simeone","sequence":"additional","affiliation":[{"name":"2 International Institute of Genetics and Biophysics, CNR, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris J. L. 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