{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T19:12:04Z","timestamp":1770491524647,"version":"3.49.0"},"reference-count":31,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1990,5,1]],"date-time":"1990-05-01T00:00:00Z","timestamp":641520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Nuclear extracts from embryos of Xenopus laevis were shown to contain a protein activity, KTF-1, which binds in vitro to the promoter of the embryonic, epidermisspecific keratin gene, XK81A1. Mobility shift assays, methylation interference and footprinting analysis were used to show that the KTF-1 binding site contains an imperfect, palindromic sequence, ACCCTGAGGCT. This sequence occurs once in the XK81A1 promoter, 152-162 base pairs upstream of the transcription start site. A construct of the keratin gene in which this sequence was altered so that it no longer binds KTF-1 in vitro showed severely reduced transcription levels upon injection into Xenopus embryos, but retained epidermal specificity. Addition of KTF-1 binding sites also enhanced epidermal and non-epidermal activity of a heterologous promoter, Xenopus \/J-globin, in embryos. These results suggest that KTF-1 is a general activator of embryonic keratin transcription, which acts in concert with other factors to produce high levels of epidermisspecific expression.<\/jats:p>","DOI":"10.1242\/dev.109.1.157","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:49:37Z","timestamp":1619398177000},"page":"157-165","source":"Crossref","is-referenced-by-count":43,"title":["KTF-1, a transcriptional activator of <i>Xenopus<\/i> embryonic keratin expression"],"prefix":"10.1242","volume":"109","author":[{"given":"A. M.","family":"Snape","sequence":"first","affiliation":[{"name":"National Institute of Child Health and Human Development Laboratory of Molecular Genetics , , National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. A.","family":"Jonas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. D.","family":"Sargent","sequence":"additional","affiliation":[{"name":"National Institute of Child Health and Human Development Laboratory of Molecular Genetics , , National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1990,5,1]]},"reference":[{"key":"2023121501472872600_DEVELOP_109_1_157C1","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel","year":"1987"},{"key":"2023121501472872600_DEVELOP_109_1_157C2","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1002\/j.1460-2075.1987.tb04855.x","article-title":"Differential promoter utilization by the bovine papillomavirus in transformed cells and productively infected wart tissues","volume":"6","author":"Baker","year":"1987","journal-title":"EMBOJ"},{"key":"2023121501472872600_DEVELOP_109_1_157C3","first-page":"567","article-title":"Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs","volume":"4","author":"Bendig","year":"1984","journal-title":"Mol. 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