{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T02:40:25Z","timestamp":1773196825680,"version":"3.50.1"},"reference-count":38,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1990,10,1]],"date-time":"1990-10-01T00:00:00Z","timestamp":654739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>There has been considerable debate about the ontological origin of gut endocrine cells as being either from the neural crest (or primitive epiblast) or from the endodermal stem cell. We have attempted to define the ontological origin of endocrine cells by applying an experimental system that uses a marker to Identify one of the two phenotypes present in chimaeric mice as suggested by Ponder et al. (1985).<\/jats:p>\n               <jats:p>This study involved two separate experiments. The first made use of the unique staining properties of Dolichos bifloras agglutinin (DBA), a lectin that binds to the A-acetyl galactosamine sugar residues present on the surface of C57B1 mouse gut, but absent from RoRIII mouse gut, in C57Bl\u2194Roin mouse chimaeras at the ultrastructural level. A four-stage procedure for staining at the EM level was developed. Although mature villous endocrine cells stained for DBA, immature endocrine cells did not, either in the positive crypts of chimaeric mouse gut or in gut from C57B1 positive controls.<\/jats:p>\n               <jats:p>Thus a second marker was chosen. This experiment combined immunocytochemistry (to identify gastric antral gastrin cells chosen as a representative neuro-endocrine cell) with in situ DNA hybridization for the mouse male chromosome repeat sequence PY 353 (to identify XY cells) in XX\u2192XY chimaeric mice. This study showed that the sex chromosomal pattern in the gastrin cells parallels that of other cells in the same gastric gland and therefore are clonal with them. This suggests that gut endocrine cells share a common stem cell with other epithelial cell lineages in the antrum and are endodermally derived.<\/jats:p>","DOI":"10.1242\/dev.110.2.477","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T01:44:15Z","timestamp":1619401455000},"page":"477-481","source":"Crossref","is-referenced-by-count":84,"title":["Gastric endocrine cells share a clonal origin with other gut cell lineages"],"prefix":"10.1242","volume":"110","author":[{"given":"E. M.","family":"Thompson","sequence":"first","affiliation":[{"name":"1 Department of Histopathology, Royal Postgraduate Medical School, London W12 OHS, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. A.","family":"Fleming","sequence":"additional","affiliation":[{"name":"University of Oxford, John Radcliffe Hospital, Oxford, UK 2 Nuffield Department of Pathology and Bacteriology ,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Evans","sequence":"additional","affiliation":[{"name":"1 Department of Histopathology, Royal Postgraduate Medical School, London W12 OHS, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Fundele","sequence":"additional","affiliation":[{"name":"3 Department of Molecular Embryology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Surani","sequence":"additional","affiliation":[{"name":"3 Department of Molecular Embryology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. A.","family":"Wright","sequence":"additional","affiliation":[{"name":"1 Department of Histopathology, Royal Postgraduate Medical School, London W12 OHS, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1990,10,1]]},"reference":[{"key":"2023050312025392000_DEVELOP_110_2_477C1","first-page":"307","article-title":"A study of the developmental relationship between enterochromaffin cells and the neural crest","volume":"11","author":"Andrew","year":"1963","journal-title":"J. Embryol exp. 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