{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:35:42Z","timestamp":1790620542829,"version":"4.1.0"},"reference-count":52,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1990,11,1]],"date-time":"1990-11-01T00:00:00Z","timestamp":657417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Laminin is a large glycoprotein of basement membranes. The best described laminin from a mouse tumor contains three polypeptide chains (A, Bl and B2), but there is recent evidence that some cell types produce, laminin isoforms lacking the A chain. We have here studied the occurrence of the isoforms during mouse organogenesis. In all tissues studied, the A chain mRNA and polypeptide were more weakly expressed than those of the B chains. Laminin A chain polypeptides showed a much more restricted tissue distribution than the B chains. Laminin A chain polypeptide was mainly detected in basement membranes of epithelial cells, suggesting that this chain is important for morphogenesis of epithelial sheets. Most endothelial basement membranes and all embryonic mesenchyme matrices studied seemed to lack the A chain even though they contained B chains. Several of the cells producing laminin devoid of A chain seem to produce other polypeptides that become complexed to the B chains. With an anti-laminin antiserum, which in immunblots reacts only with A and B polypeptide chains, additional polypeptides of 160 and 190\u00d7l03Mr were co-precipitated from all tissues studied. In developing heart, a polypeptide of 300\u00d7l03Mr was co-precipitated in addition. Our data suggest that these laminin-associated polypeptides are not formed by a differential splicing of the known A chain mRNA. Northern blotting of poly(A)+ RNA showed only 10 kb A chain transcripts but no truncated forms. We conclude that several cell types in the mouse embryo produce laminin variants that lack the 400\u00d7l03Mr A chain. Since a major cell binding site of laminin contains parts of the A chain, the variants should differ in biological function from laminin containing this A chain.<\/jats:p>","DOI":"10.1242\/dev.110.3.823","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T01:38:59Z","timestamp":1619401139000},"page":"823-837","source":"Crossref","is-referenced-by-count":116,"title":["Differential expression of laminin A and B chains during development of embryonic mouse organs"],"prefix":"10.1242","volume":"110","author":[{"given":"Gerd","family":"Klein","sequence":"first","affiliation":[{"name":"1 Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, D-7400 T\u00fcbingen, Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marja","family":"Ekblom","sequence":"additional","affiliation":[{"name":"1 Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, D-7400 T\u00fcbingen, Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lothar","family":"Fecker","sequence":"additional","affiliation":[{"name":"1 Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, D-7400 T\u00fcbingen, Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupert","family":"Timpl","sequence":"additional","affiliation":[{"name":"2 Max-Planck-Institut f\u00fcr Biochemie, D-8033 Martinsried, Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Ekblom","sequence":"additional","affiliation":[{"name":"1 Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, D-7400 T\u00fcbingen, Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1990,11,1]]},"reference":[{"key":"2024071007210171900_DEVELOP_110_3_823C1","doi-asserted-by":"crossref","first-page":"16163","DOI":"10.1016\/S0021-9258(18)37573-2","article-title":"Enhanced synthesis and secretion of type IV collagen and entactin during adipose conversion of 3T3-L1 cells and production of unorthodox laminin complex","volume":"263","author":"Aratani","year":"1988","journal-title":"J. biol. Chem"},{"key":"2024071007210171900_DEVELOP_110_3_823C2","doi-asserted-by":"crossref","first-page":"11532","DOI":"10.1016\/S0021-9258(18)60840-3","article-title":"The cellular interactions with laminin. Cell adhesion correlates with two fragment specific high affinity binding sites","volume":"262","author":"Aumailley","year":"1987","journal-title":"J. biol. Chem"},{"key":"2024071007210171900_DEVELOP_110_3_823C3","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0014-4827(90)90277-H","article-title":"Antibody to integrin o6 subunit specifically inhibits cell-binding to laminin fragment 8","volume":"188","author":"Aumailley","year":"1990","journal-title":"Expl Cell Res"},{"key":"2024071007210171900_DEVELOP_110_3_823C4","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1073\/pnas.69.6.1408","article-title":"Purification of biologically active globin messenger RNA by chromatography on oligotymidylic acid cellulose","volume":"69","author":"Aviv","year":"1972","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2024071007210171900_DEVELOP_110_3_823C5","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1002\/j.1460-2075.1984.tb02140.x","article-title":"Sequencing of laminin B chain cDNAs reveals C-terminal regions of coiled-coil alpha helix","volume":"3","author":"Barlow","year":"1984","journal-title":"EMBO J"},{"key":"2024071007210171900_DEVELOP_110_3_823C6","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1096\/fasebj.4.2.2404817","article-title":"Structure and function of laminin: anatomy of a multidomain glycoprotein","volume":"4","author":"Beck","year":"1990","journal-title":"FASEB J"},{"key":"2024071007210171900_DEVELOP_110_3_823C7","volume-title":"A Textbook of Histology","author":"Bloom","year":"1975"},{"key":"2024071007210171900_DEVELOP_110_3_823C8","doi-asserted-by":"crossref","first-page":"5294","DOI":"10.1021\/bi00591a005","article-title":"Isolation of biologically active ribonucleic acid from sources enriched in ribonucleases","volume":"18","author":"Chirgwin","year":"1979","journal-title":"Biochemistry"},{"key":"2024071007210171900_DEVELOP_110_3_823C9","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/0012-1606(83)90183-5","article-title":"Subunits of laminin are differentially synthesized in mouse eggs and early embryos","volume":"96","author":"Cooper","year":"1983","journal-title":"Devi Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C10","doi-asserted-by":"crossref","first-page":"3850","DOI":"10.1073\/pnas.80.12.3850","article-title":"In vivo and in vitro observations of laminin production by Schwann cells","volume":"80","author":"Cornbrooks","year":"1983","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2024071007210171900_DEVELOP_110_3_823C11","doi-asserted-by":"crossref","DOI":"10.1111\/j.1432-1033.1990.tb19151.x","article-title":"Cell adhesion, spreading and neurite stimulation by laminin fragment E8 depends on maintenance of secondary and tertiary structure in its rod and globular domain","volume-title":"Eur. J. Biochem. (in press)","author":"Deutzmann","year":"1990"},{"key":"2024071007210171900_DEVELOP_110_3_823C12","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/j.1432-1033.1988.tb14342.x","article-title":"Structural study of long arm fragments of laminin. Evidence for repetitive \u03b2-terminal sequences in the A-chain, not present in the B-chains","volume":"177","author":"Deutzmann","year":"1988","journal-title":"Eur. J. Biochem"},{"key":"2024071007210171900_DEVELOP_110_3_823C13","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1111\/j.1432-0436.1986.tb00582.x","article-title":"Control of laminin synthesis during differentiation of F9 embryonal carcinoma cells","volume":"32","author":"Durkin","year":"1986","journal-title":"Differentiation"},{"key":"2024071007210171900_DEVELOP_110_3_823C14","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1083\/jcb.106.4.1299","article-title":"Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies","volume":"106","author":"Edgar","year":"1988","journal-title":"J. Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C15","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0092-8674(90)90748-4","article-title":"Transient and locally restricted expression of laminin A chain mRNA by developing epithelial cells during kidney organogenesis","volume":"60","author":"Ekblom","year":"1990","journal-title":"Cell"},{"key":"2024071007210171900_DEVELOP_110_3_823C16","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1096\/fasebj.3.10.2666230","article-title":"Developmentally regulated conversion of mesenchyme to epithelium","volume":"3","author":"Ekblom","year":"1989","journal-title":"FASEB J"},{"key":"2024071007210171900_DEVELOP_110_3_823C17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0014-5793(89)81417-6","article-title":"EGF-like domains in extracellular matrix proteins - localized signals for growth and differentiation?","volume":"251","author":"Engel","year":"1989","journal-title":"FEBS Lett"},{"key":"2024071007210171900_DEVELOP_110_3_823C18","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0022-2836(81)90326-0","article-title":"Shapes, domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix","volume":"150","author":"Engel","year":"1981","journal-title":"J. molec. Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C19","first-page":"5193","article-title":"Biosynthesis and secretion of laminin and laminin-associated glycoproteins by non-malignant and malignant human k\u00e9ratinocytes: companson of cell lines from primary and secondary tumors in the same patient","volume":"48","author":"Frenette","year":"1988","journal-title":"Cancer Res"},{"key":"2024071007210171900_DEVELOP_110_3_823C20","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1083\/jcb.105.1.589","article-title":"Two distinct cell-binding domains in laminin can independently promote non-neural cell adhesion and spreading","volume":"105","author":"Goodman","year":"1987","journal-title":"J. Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C21","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/0092-8674(89)90945-8","article-title":"Two different laminin domains mediate the differentiation of human endothelial cells into capillary-like structures in vitro","volume":"59","author":"Grant","year":"1989","journal-title":"Cell"},{"key":"2024071007210171900_DEVELOP_110_3_823C22","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1242\/dev.107.2.281","article-title":"Antisera to basal lamina and glial endfeet disturb the normal extension of axons on retina and pigment epithelium basal laminae","volume":"107","author":"Haleter","year":"1989","journal-title":"Development"},{"key":"2024071007210171900_DEVELOP_110_3_823C23","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/j.1432-1033.1988.tb14045.x","article-title":"The N terminus of laminin A chain is homologous to the B chains","volume":"173","author":"Hartl","year":"1988","journal-title":"Eur. J. Biochem"},{"key":"2024071007210171900_DEVELOP_110_3_823C24","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1083\/jcb.107.1.307","article-title":"Diverse effects of fibronectin and laminin on phenotypic properties of cultured artertial smooth muscle cells","volume":"107","author":"Hedin","year":"1988","journal-title":"J. Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C25","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1083\/jcb.106.4.1365","article-title":"Differential effect of laminin, intact type IV collagen, and specific domains of type IV collagen on endothelial cell adhesion and migration","volume":"106","author":"Herbst","year":"1988","journal-title":"J Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C26","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/338229a0","article-title":"A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction","volume":"338","author":"Hunter","year":"1989","journal-title":"Nature"},{"key":"2024071007210171900_DEVELOP_110_3_823C27","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/0092-8674(88)90056-6","article-title":"Role of laminin A chain in the development of epithelial cell polarity","volume":"55","author":"Klein","year":"1988","journal-title":"Cell"},{"key":"2024071007210171900_DEVELOP_110_3_823C28","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1002\/jcb.240270402","article-title":"Biological activities of laminin","volume":"27","author":"Kleinman","year":"1985","journal-title":"J. cell. Biochem"},{"key":"2024071007210171900_DEVELOP_110_3_823C29","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/0012-1606(87)90302-2","article-title":"Genes for basement membrane proteins are coordinately expressed in differentiating F9 cells but not in normal adult murine tissues","volume":"122","author":"Kleinman","year":"1987","journal-title":"Devi Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C30","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/0012-1606(82)90122-1","article-title":"Synthesis of type IV collagen and laminin in cultures of skeletal muscle cells and their assembly on the surface of myotubes","volume":"93","author":"Kuhl","year":"1982","journal-title":"Devi Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C31","doi-asserted-by":"crossref","first-page":"2455","DOI":"10.1083\/jcb.109.5.2455","article-title":"Endothelial cells use o\u20192\u03b21 integrin as a laminin receptor","volume":"109","author":"Languino","year":"1989","journal-title":"J. Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C32","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/BF00239443","article-title":"Embryonic chicken retinal cells can regenerate all cell layers in vitro, but ciliary pigmented cells induce their correct polarity","volume":"258","author":"Layer","year":"1989","journal-title":"Cell Tissue Res"},{"key":"2024071007210171900_DEVELOP_110_3_823C33","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis","year":"1982"},{"key":"2024071007210171900_DEVELOP_110_3_823C34","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/j.1432-1033.1988.tb14430.x","article-title":"Characterization of proteolytic fragments of the laminin-nidogen complex and their activity in ligand-binding assays","volume":"178","author":"Mann","year":"1988","journal-title":"Eur. J. Biochem"},{"key":"2024071007210171900_DEVELOP_110_3_823C35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1146\/annurev.cb.03.110187.000421","article-title":"Laminin and other basement membrane components","volume":"3","author":"Martin","year":"1987","journal-title":"A. Rev. Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C36","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/0092-8674(88)90166-3","article-title":"Drosophila substrate adhesion molecule: sequence of laminin Bl chain reveals domain homology with mouse","volume":"53","author":"Montell","year":"1988","journal-title":"Cell"},{"key":"2024071007210171900_DEVELOP_110_3_823C37","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1083\/jcb.109.5.2441","article-title":"Drosophila laminin: sequence of B2 subunit and expression of all three subunits during embryogenesis","volume":"109","author":"Montell","year":"1989","journal-title":"J. Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C38","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0378-1119(86)90387-2","article-title":"New pUC derived expression vectors for rapid construction of cDNA libraries","volume":"49","author":"Oberb\u00e2umer","year":"1986","journal-title":"Gene"},{"key":"2024071007210171900_DEVELOP_110_3_823C39","first-page":"772","article-title":"Human laminin: cloning and sequence analysis of cDNAs encoding A, Bl and B2 chains, and expression of the corresponding genes in human skin and cultured cells","volume":"60","author":"Olsen","year":"1989","journal-title":"Lab. Invest"},{"key":"2024071007210171900_DEVELOP_110_3_823C40","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1432-1033.1987.tb13476.x","article-title":"Laminin-nidogen complex. Extraction with chelating agents and structural chracterization","volume":"166","author":"Paulsson","year":"1987","journal-title":"Eur. J. Biochem"},{"key":"2024071007210171900_DEVELOP_110_3_823C41","doi-asserted-by":"crossref","first-page":"18726","DOI":"10.1016\/S0021-9258(18)51528-3","article-title":"Mouse heart laminin: Purification of the native protein and structural comparison with EHS tumor laminin","volume":"264","author":"Paulsson","year":"1989","journal-title":"J. biol. Chem"},{"key":"2024071007210171900_DEVELOP_110_3_823C42","doi-asserted-by":"crossref","first-page":"14 732","DOI":"10.1016\/S0021-9258(17)38634-9","article-title":"The biosynthesis, processing and secretion of laminin by human choriocarcinoma cells","volume":"260","author":"Peters","year":"1985","journal-title":"J. biol. Chem"},{"key":"2024071007210171900_DEVELOP_110_3_823C43","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/330068a0","article-title":"Retinal pigmented epithelial cells induced to transdifferentiate to neurons by laminin","volume":"330","author":"Reh","year":"1987","journal-title":"Nature"},{"key":"2024071007210171900_DEVELOP_110_3_823C44","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1083\/jcb.108.3.1127","article-title":"New mammary epithelial and fibroblastic cell clones in coculture form structures competent to differentiate functionally","volume":"108","author":"Reichmann","year":"1989","journal-title":"J. Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C45","doi-asserted-by":"crossref","first-page":"16536","DOI":"10.1016\/S0021-9258(18)37424-6","article-title":"Laminin, a multidomain protein. The A chain has a unique globular domain and homology with the basement membrane proteoglycan and the laminin B chains","volume":"263","author":"Sasaki","year":"1988","journal-title":"J. biol. Chem"},{"key":"2024071007210171900_DEVELOP_110_3_823C46","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1242\/dev.104.3.431","article-title":"The localization of laminin mRNA and protein in the postimplantation embryo and placenta of the mouse: an in situ hybridization and immunocytocheimcal study","volume":"104","author":"Senior","year":"1988","journal-title":"Development"},{"key":"2024071007210171900_DEVELOP_110_3_823C47","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1242\/dev.102.2.339","article-title":"Epithelial-mesenchymal interactions in the production of basement membrane components in the gut","volume":"102","author":"Simon-Assmann","year":"1988","journal-title":"Development"},{"key":"2024071007210171900_DEVELOP_110_3_823C48","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1083\/jcb.110.6.2145","article-title":"Integrin recognition of different cell-binding fragments of laminin (Pl, E3, E8) and evidence that a6\/?l but not n6\u03b24 functions as a major receptor for fragment E8","volume":"110","author":"Sonnenberg","year":"1990","journal-title":"J. Cell Biol"},{"key":"2024071007210171900_DEVELOP_110_3_823C49","doi-asserted-by":"crossref","first-page":"5201","DOI":"10.1073\/pnas.77.9.5201","article-title":"Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose","volume":"77","author":"Thomas","year":"1980","journal-title":"Proc. natn. Acad. Sci. USA"},{"key":"2024071007210171900_DEVELOP_110_3_823C50","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/j.1432-1033.1989.tb14673.x","article-title":"Structure and biological activity of basement membrane proteins","volume":"180","author":"Timpl","year":"1989","journal-title":"Eur. J. Biochem"},{"key":"2024071007210171900_DEVELOP_110_3_823C51","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1177\/37.2.2463302","article-title":"Method for epitope selection of antisera for immunohistology","volume":"37","author":"Wedlich","year":"1989","journal-title":"J. Histochem. Cytochem"},{"key":"2024071007210171900_DEVELOP_110_3_823C52","volume-title":"Tissue Interactions and Development","author":"Wessells","year":"1977"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/110\/3\/823\/3540176\/develop_110_3_823.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/110\/3\/823\/3540176\/develop_110_3_823.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T07:21:49Z","timestamp":1720596109000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/110\/3\/823\/36969\/Differential-expression-of-laminin-A-and-B-chains"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11,1]]},"references-count":52,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,11,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.110.3.823","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,11,1]]}}}